• Product name

  • Description

    Rabbit polyclonal to CHMP6/VPS20
  • Host species

  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Cow, Orangutan
  • Immunogen

    Synthetic peptide corresponding to Human CHMP6/VPS20 aa 1-100 (N terminal) conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab90851)

  • Positive control

    • Recombinant Human CHMP6/VPS20 protein (ab124606) can be used as a positive control in WB. This antibody gave a positive signal in the following whole cell lysates: Hela; Jurkat; HepG2; HEK293; MCF7; JEG3; K562.
  • General notes

     This product was previously labelled as CHMP6




Our Abpromise guarantee covers the use of ab76929 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 23 kDa).


  • Function

    Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) which is involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. The MVB pathway appears to require the sequential function of ESCRT-O, -I,-II and -III complexes. ESCRT-III proteins mostly dissociate from the invaginating membrane before the ILV is released. The ESCRT machinery also functions in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis and the budding of enveloped viruses (HIV-1 and other lentiviruses). ESCRT-III proteins are believed to mediate the necessary vesicle extrusion and/or membrane fission activities, possibly in conjunction with the AAA ATPase VPS4. In the ESCRT-III complex, it probably serves as an acceptor for the ESCRT-II complex on endosomal membranes.
  • Tissue specificity

    Ubiquitously expressed.
  • Sequence similarities

    Belongs to the SNF7 family.
  • Domain

    The acidic C-terminus and the basic N-termminus are thought to render the protein in a closed, soluble and inactive conformation through an autoinhibitory intramolecular interaction. The open and active conformation, which enables membrane binding and oligomerization, is achieved by interaction with other cellular binding partners, probably including other ESCRT components.
  • Cellular localization

    Endomembrane system. Endosome membrane. Late endosome membrane. Localizes to endosomal membranes.
  • Information by UniProt
  • Database links

  • Alternative names

    • Charged multivesicular body protein 6 antibody
    • chmp6 antibody
    • CHMP6_HUMAN antibody
    • Chromatin modifying protein 6 antibody
    • Chromatin-modifying protein 6 antibody
    • FLJ11749 antibody
    • hVps 20 antibody
    • hVps20 antibody
    • novel SNF7 protein antibody
    • OTTMUSP00000004363 antibody
    • RGD1565325 antibody
    • RP24 225K18.4 antibody
    • Vacuolar protein sorting associated protein 20 antibody
    • Vacuolar protein sorting-associated protein 20 antibody
    • Vps 20 antibody
    • Vps20 antibody
    see all


  • All lanes : Anti-CHMP6/VPS20 antibody (ab76929) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 4 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
    Lane 5 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
    Lane 6 : JEG-3 (Human placental choriocarcinoma cell line) Whole Cell Lysate
    Lane 7 : K562 (Human erythromyeloblastoid leukemia cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 23 kDa
    Observed band size: 50 kDa
    why is the actual band size different from the predicted?

    Exposure time: 30 seconds

    This protein contains a N-myristoyl glycine amino acid modification that we believe might be responsible for the size shift observed. Furthermore, the 50 kDa band observed is comparable to the molecular weight shown in scientific literature and obtained with other commercially available antibodies to CHMP6/VPS20. Abcam would love to hear from anyone that can help explain the results obtained.


ab76929 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Immunocytochemistry/ Immunofluorescence
Human Cell (HeLa)
Yes - 0.5% Triton X100

Dr. Kirk Mcmanus

Verified customer

Submitted Aug 03 2011

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