Key features and details
- Rabbit polyclonal to CHN 1
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-CHN 1 antibody
See all CHN 1 primary antibodies
DescriptionRabbit polyclonal to CHN 1
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse
Recombinant fragment corresponding to Human CHN 1 aa 1-200.
Database link: P15882
- WB: HEK-293T, MCF7, HeLa and Jurkat whole cell lysates. IHC-P: Human colon cancer tissue.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab229929 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/5000. Detects a band of approximately 53 kDa (predicted molecular weight: 54, 39, 51 kDa).|
|IHC-P||1/20 - 1/200.|
FunctionGTPase-activating protein for p21-rac and a phorbol ester receptor. May play an important role in neuronal signal-transduction mechanisms.
Tissue specificityIn neurons in brain regions that are involved in learning and memory processes.
Involvement in diseaseDefects in CHN1 are the cause of Duane retraction syndrome type 2 (DURS2) [MIM:604356]. Duane retraction syndrome is a congenital eye movement disorder characterized by a failure of cranial nerve VI (the abducens nerve) to develop normally, resulting in restriction or absence of abduction, adduction, or both, and narrowing of the palpebral fissure and retraction of the globe on attempted adduction. Undiagnosed in children, it can lead to amblyopia, a permanent uncorrectable loss of vision.
Sequence similaritiesContains 1 phorbol-ester/DAG-type zinc finger.
Contains 1 Rho-GAP domain.
Contains 1 SH2 domain.
Developmental stageIncreases in amount during brain development coincident with synaptogenesis.
- Information by UniProt
- A-chimaerin antibody
- Alpha-chimerin antibody
- ARHGAP2 antibody
All lanes : Anti-CHN 1 antibody (ab229929) at 1/1000 dilution
Lane 1 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : MCF7 (Human breast adenocarcinoma cell line) whole cell lysate
Lane 3 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 4 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
All lanes : Goat polyclonal to Rabbit IgG at 1/10000 dilution
Developed using the ECL technique.
Predicted band size: 54, 39, 51 kDa
Paraffin-embedded human colon cancer tissue stained for CHN 1 with ab229929 at a 1/100 dilution in immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab229929 has not yet been referenced specifically in any publications.