Overview

  • Product name

    CHO HCP ELISA kit (Host cell protein)
  • Detection method

    Colorimetric
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    10 ng/ml
  • Range

    3.3 ng/ml - 810 ng/ml
  • Assay time

    3h 0m
  • Assay duration

    Multiple steps standard assay
  • Product overview

    CHO HCP ELISA kit (Host cell protein) (ab240996) is designed to quantitatively measure host cell protein (HCP) contamination in bulk products expressed in Chinese Hamster Ovary (CHO) expression systems.


    The provided 96-well plate has been pre-coated with Rabbit anti-CHO HCP antibodies to capture CHO proteins from biological samples. Following an incubation to allow capture of the CHO protein by the antibodies on the plate, a second anti-CHO HCP antibody, conjugated with biotin, is added and incubated to allow it to bind to the captured CHO proteins. After incubation, the plate is washed and a Streptavidin-HRP conjugate is added and incubated for 30 min. The Streptavidin-HRP conjugate will be captured by any biotin labeled antibody bound to the plate. Following a wash step to remove unbound conjugate, TMB substrate is added and is converted by the captured HRP to a colored product in proportion to the amount of HCP bound to the plate. After a short incubation to allow color development, the reaction is stopped and the intensity of the generated color is detected in a spectrophotometer plate reader capable of measuring 450nm wavelength.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    10X PBST 1 x 30ml
    5X Dilution Buffer 1 x 15ml
    CHO Protein Standard 1 x 600µl
    Coated Clear 96 Well Plate 1 unit
    Plate Sealer 1 unit
    Reporting Antibody 1 x 90µl
    Stop Solution 1 x 15ml
    Streptavidin-HRP Conjugate 1 x 420µl
    TMB Substrate 1 x 15ml
  • Relevance

    Chinese Hamster Ovary Cell line is used for antibody production and in recombinant protein expression methods.
  • Alternative names

    • Chinese Hamster Ovary Cell line

Applications

Our Abpromise guarantee covers the use of ab240996 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • The standard curve was prepared as described in Section 10.

     

Protocols

References

ab240996 has not yet been referenced specifically in any publications.

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