Overview

  • Product name

    Anti-CHODL antibody [EPR8757]
    See all CHODL primary antibodies
  • Description

    Rabbit monoclonal [EPR8757] to CHODL
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, Flow Cytmore details
    Unsuitable for: ICC/IF,IHC-P or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human CHODL aa 50-150 (extracellular). The exact sequence is proprietary.

  • Positive control

    • Saos2, PC3, Jurkat and K562 cell lysates. Jurkat cells
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C.
  • Dissociation constant (KD)

    KD = 8.59 x 10 -11 M
    Learn more about KD
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR8757
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab134924 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 36 kDa.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

  • Application notes
    Is unsuitable for ICC/IF,IHC-P or IP.
  • Target

    • Tissue specificity

      Found in spleen, testis, prostate and fetal liver. Expression limited to vascular muscle of testis, smooth muscle of prostate stroma, heart muscle, skeletal muscle, crypts of small intestine, and red pulp of spleen.
    • Sequence similarities

      Contains 1 C-type lectin domain.
    • Post-translational
      modifications

      N-glycosylated.
    • Cellular localization

      Membrane.
    • Information by UniProt
    • Database links

    • Alternative names

      • C21orf68 antibody
      • Chodl antibody
      • CHODL_HUMAN antibody
      • Chondrolectin antibody
      • Chromosome 21 open reading frame 68 antibody
      • FLJ12627 antibody
      • MT75 antibody
      • PRED12 antibody
      • Transmembrane protein MT75 antibody
      • UNQ872/PRO1890 antibody
      see all

    Images

    • All lanes : Anti-CHODL antibody [EPR8757] (ab134924) at 1/1000 dilution

      Lane 1 : Saos2 cell lysate
      Lane 2 : PC3 cell lysate
      Lane 3 : Jurkat cell lysate
      Lane 4 : K562 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat-anti-rabbit HRP at 1/2000 dilution

      Developed using the ECL technique.

      Predicted band size: 36 kDa

    • Flow cytometric analysis of Jurkat cells using ab134924 at 1/10 dilution staining CHODL (red) or a nonspecific rabbit IgG (green).
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    This product has been referenced in:

    • Ackermann AM  et al. Integration of ATAC-seq and RNA-seq identifies human alpha cell and beta cell signature genes. Mol Metab 5:233-44 (2016). Human . Read more (PubMed: 26977395) »
    See 1 Publication for this product

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab134924.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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