Overview

  • Product name

    Anti-Cholecystokinin antibody
    See all Cholecystokinin primary antibodies
  • Description

    Rabbit polyclonal to Cholecystokinin
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, ICC/IF, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human Cholecystokinin aa 50 to the C-terminus (N terminal). The exact sequence is proprietary.
    Database link: P06307

  • Positive control

    • Stomach. This antibody gave a positive result when used in the following formaldehyde fixed cell lines: CaCO2

Properties

Applications

Our Abpromise guarantee covers the use of ab27441 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/200. Do not perform antigen retrieval. Incubate for 10 minutes at room temperature.
ICC/IF Use a concentration of 10 µg/ml.
WB Use at an assay dependent concentration. Predicted molecular weight: 12 kDa.

Target

  • Function

    This peptide hormone induces gall bladder contraction and the release of pancreatic enzymes in the gut. Its function in the brain is not clear. Binding to CCK-A receptors stimulates amylase release from the pancreas, binding to CCK-B receptors stimulates gastric acid secretion.
  • Sequence similarities

    Belongs to the gastrin/cholecystokinin family.
  • Post-translational
    modifications

    The precursor is cleaved by proteases to produce a number of active cholecystokinins.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Database links

  • Alternative names

    • (1-49)-CCK58 antibody
    • CCK antibody
    • CCK12 antibody
    • CCK18 antibody
    • CCK25 antibody
    • CCK33 antibody
    • CCK39 antibody
    • CCK5 antibody
    • CCK58 antibody
    • CCK7 antibody
    • CCK8 antibody
    • CCKN_HUMAN antibody
    • Cholecystokinin-5 antibody
    see all

Images

  • ICC/IF image of ab27441 stained CaCO2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab27441 at 10µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Ab27441, at a dilution of 1/200, staining Cholecystokinin in formalin fixed, paraffin embedded stomach tissue by Immunohistochemistry.

  • Anti-Cholecystokinin antibody (ab27441) at 1/25 dilution + Brain cell lysate

    Predicted band size: 12 kDa
    Observed band size: 12 kDa

References

This product has been referenced in:

  • Ye DZ & Kaestner KH Foxa1 and Foxa2 control the differentiation of goblet and enteroendocrine L- and D-cells in mice. Gastroenterology 137:2052-62 (2009). IHC-P ; Mouse . Read more (PubMed: 19737569) »
See 1 Publication for this product

Customer reviews and Q&As

Answer

I just received some extra information from the originator of this product. They have confirmed that this antibody does not require antigen retrieval,and the recommended dilution is 1:200,10min at RT. However, these condition may not match with Ventana-XT. Ventana-XT has many advantages over other automation machines. But it will automatically dilute primary antibody before incubation. And a few antibodies don't work with Ventana-XT. Therefore, it is suggested that: 1. try dilution at 1:25-1:50, 10min at 37 degree Celsius (default temperature on Ventana-XT). 2. increase the secondary antibody incubation time. I hope this extra information will be helpful.

Read More

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up