Key features and details
- Rabbit polyclonal to Cholecystokinin
- Suitable for: IHC-P, ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Cholecystokinin antibody
See all Cholecystokinin primary antibodies
DescriptionRabbit polyclonal to Cholecystokinin
Tested applicationsSuitable for: IHC-P, ICC/IF, WBmore details
Species reactivityReacts with: Human
Synthetic peptide within Human Cholecystokinin aa 50 to the C-terminus (N terminal). The exact sequence is proprietary.
Database link: P06307
- Stomach. This antibody gave a positive result when used in the following formaldehyde fixed cell lines: CaCO2
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.60
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab27441 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/200. Do not perform antigen retrieval. Incubate for 10 minutes at room temperature.|
|ICC/IF||Use a concentration of 10 µg/ml.|
|WB||Use at an assay dependent concentration. Predicted molecular weight: 12 kDa.|
FunctionThis peptide hormone induces gall bladder contraction and the release of pancreatic enzymes in the gut. Its function in the brain is not clear. Binding to CCK-A receptors stimulates amylase release from the pancreas, binding to CCK-B receptors stimulates gastric acid secretion.
Sequence similaritiesBelongs to the gastrin/cholecystokinin family.
modificationsThe precursor is cleaved by proteases to produce a number of active cholecystokinins.
- Information by UniProt
- (1-49)-CCK58 antibody
- CCK antibody
- CCK12 antibody
ICC/IF image of ab27441 stained CaCO2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab27441 at 10µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Ab27441, at a dilution of 1/200, staining Cholecystokinin in formalin fixed, paraffin embedded stomach tissue by Immunohistochemistry.
Anti-Cholecystokinin antibody (ab27441) at 1/25 dilution + Brain cell lysate
Predicted band size: 12 kDa
Observed band size: 12 kDa
ab27441 has been referenced in 1 publication.
- Ye DZ & Kaestner KH Foxa1 and Foxa2 control the differentiation of goblet and enteroendocrine L- and D-cells in mice. Gastroenterology 137:2052-62 (2009). IHC-P ; Mouse . PubMed: 19737569