• Product name

    Anti-Cholera Toxin antibody
    See all Cholera Toxin primary antibodies
  • Description

    Rabbit polyclonal to Cholera Toxin
  • Host species

  • Tested applications

    Suitable for: WB, ELISAmore details
  • Species reactivity

    Reacts with: Other species
  • Immunogen

    Cholera toxin and the toxoid purified from culture medium of Vibrio cholerae 569B strain.

  • Positive control

    • Culture medium of Vibrio cholerae 569B strain


  • Form

  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.05% Sodium azide
    Constituent: 100% Whole serum
  • Purity

    Whole antiserum
  • Clonality

  • Isotype

  • Research areas


Our Abpromise guarantee covers the use of ab123129 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 14, 29 kDa.
ELISA Use at an assay dependent concentration.


  • Relevance

    The holotoxin (choleragen) consists of a pentameric ring of B subunits whose central pore is occupied by the A subunit. The A subunit contains two chains, A1 and A2, linked by a disulfide bridge. The B subunit pentameric ring directs the A subunit to its target by binding to the GM1 gangliosides present on the surface of the intestinal epithelial cells. It can bind five GM1 gangliosides. It has no toxic activity by itself. After binding to gangliosides GM1 in lipid rafts, through the subunit B pentamer, the holotoxin and the gangliosides are internalized. The holotoxin remains bound to GM1 until arrival in the ER. The A subunit has previously been cleaved in the intestinal lumen but the A1 and A2 chains have remained associated. In the ER, the A subunit disulfide bridge is reduced, the A1 chain is unfolded by the PDI and disassembled from the rest of the toxin. Then, the membrane-associated ER oxidase ERO1 oxidizes PDI, which releases the unfolded A1 chain. The next step is the retrotranslocation of A1 into the cytosol. This might be mediated by the protein-conducting pore SEC61. Upon arrival in the cytosol, A1 refolds and avoids proteasome degradation. In one way or another, A1 finally reaches its target and induces toxicity.
  • Cellular localization

  • Alternative names

    • ctxA antibody
    • Cholera enterotoxin B chain antibody
    • Cholera enterotoxin gamma chain antibody
    • Cholera enterotoxin subunit A antibody
    • Cholera enterotoxin subunit B antibody
    • Choleragenoid antibody
    • ctxA antibody
    • ctxB antibody
    • toxA antibody
    • toxB antibody
    see all


  • Anti-Cholera Toxin antibody (ab123129) at 1/2000 dilution + Culture medium of Vibrio cholerae 569B strain

    Predicted band size: 14, 29 kDa

  • Culture medium of Vibrio cholerae 569B strain subjected to electrophoresis under reducing condition followed by silver-staining.


This product has been referenced in:

  • Rasti ES & Brown AC Cholera Toxin Encapsulated within Several Vibrio cholerae O1 Serotype Inaba Outer Membrane Vesicles Lacks a Functional B-Subunit. Toxins (Basel) 11:N/A (2019). Read more (PubMed: 30959895) »
See 1 Publication for this product

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