Key features and details
- Rabbit polyclonal to Cholera Toxin
- Suitable for: WB, ELISA
- Isotype: IgG
Product nameAnti-Cholera Toxin antibody
See all Cholera Toxin primary antibodies
DescriptionRabbit polyclonal to Cholera Toxin
Tested applicationsSuitable for: WB, ELISAmore details
Species reactivityReacts with: Other species
Cholera toxin and the toxoid purified from culture medium of Vibrio cholerae 569B strain.
- Culture medium of Vibrio cholerae 569B strain
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 6
Preservative: 0.05% Sodium azide
Constituent: Whole serum
Concentration information loading...
Our Abpromise guarantee covers the use of ab123129 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration. Predicted molecular weight: 14, 29 kDa.|
|ELISA||Use at an assay dependent concentration.|
RelevanceThe holotoxin (choleragen) consists of a pentameric ring of B subunits whose central pore is occupied by the A subunit. The A subunit contains two chains, A1 and A2, linked by a disulfide bridge. The B subunit pentameric ring directs the A subunit to its target by binding to the GM1 gangliosides present on the surface of the intestinal epithelial cells. It can bind five GM1 gangliosides. It has no toxic activity by itself. After binding to gangliosides GM1 in lipid rafts, through the subunit B pentamer, the holotoxin and the gangliosides are internalized. The holotoxin remains bound to GM1 until arrival in the ER. The A subunit has previously been cleaved in the intestinal lumen but the A1 and A2 chains have remained associated. In the ER, the A subunit disulfide bridge is reduced, the A1 chain is unfolded by the PDI and disassembled from the rest of the toxin. Then, the membrane-associated ER oxidase ERO1 oxidizes PDI, which releases the unfolded A1 chain. The next step is the retrotranslocation of A1 into the cytosol. This might be mediated by the protein-conducting pore SEC61. Upon arrival in the cytosol, A1 refolds and avoids proteasome degradation. In one way or another, A1 finally reaches its target and induces toxicity.
- ctxA antibody
- Cholera enterotoxin B chain antibody
- Cholera enterotoxin gamma chain antibody
ab123129 has been referenced in 2 publications.
- Rasti ES & Brown AC Cholera Toxin Encapsulated within Several Vibrio cholerae O1 Serotype Inaba Outer Membrane Vesicles Lacks a Functional B-Subunit. Toxins (Basel) 11:N/A (2019). PubMed: 30959895
- Hubbard TP et al. A live vaccine rapidly protects against cholera in an infant rabbit model. Sci Transl Med 10:N/A (2018). PubMed: 29899024