Overview

  • Product name

    Anti-Cholera Toxin antibody
    See all Cholera Toxin primary antibodies
  • Description

    Rabbit polyclonal to Cholera Toxin
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ELISAmore details
  • Species reactivity

    Reacts with: Species independent
  • Immunogen

    Purified Choleragenoid (beta subunit).

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer

    pH: 7.20
    Preservative: 0.1% Sodium azide
    Constituent: 0.0268% PBS
  • Concentration information loading...
  • Purity

    IgG fraction
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab51572 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
  • Application notes
    ELISA: Titre 1/2000.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Relevance

      The holotoxin (choleragen) consists of a pentameric ring of B subunits whose central pore is occupied by the A subunit. The A subunit contains two chains, A1 and A2, linked by a disulfide bridge. The B subunit pentameric ring directs the A subunit to its target by binding to the GM1 gangliosides present on the surface of the intestinal epithelial cells. It can bind five GM1 gangliosides. It has no toxic activity by itself. After binding to gangliosides GM1 in lipid rafts, through the subunit B pentamer, the holotoxin and the gangliosides are internalized. The holotoxin remains bound to GM1 until arrival in the ER. The A subunit has previously been cleaved in the intestinal lumen but the A1 and A2 chains have remained associated. In the ER, the A subunit disulfide bridge is reduced, the A1 chain is unfolded by the PDI and disassembled from the rest of the toxin. Then, the membrane-associated ER oxidase ERO1 oxidizes PDI, which releases the unfolded A1 chain. The next step is the retrotranslocation of A1 into the cytosol. This might be mediated by the protein-conducting pore SEC61. Upon arrival in the cytosol, A1 refolds and avoids proteasome degradation. In one way or another, A1 finally reaches its target and induces toxicity.
    • Cellular localization

      Secreted
    • Alternative names

      • ctxA antibody
      • Cholera enterotoxin B chain antibody
      • Cholera enterotoxin gamma chain antibody
      • Cholera enterotoxin subunit A antibody
      • Cholera enterotoxin subunit B antibody
      • Choleragenoid antibody
      • ctxA antibody
      • ctxB antibody
      • toxA antibody
      • toxB antibody
      see all

    References

    ab51572 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Application
    Immunohistochemistry (Frozen sections)
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 21°C
    Sample
    Rat Tissue sections (Dorsal Root Ganglion)
    Specification
    Dorsal Root Ganglion
    Permeabilization
    Yes - PBS-Tx
    Fixative
    Paraformaldehyde

    Mr. Matthew Thakur

    Verified customer

    Submitted Feb 28 2014

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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