Product nameCholesterol Assay Kit - HDL and LDL/VLDL
See all HDL LDL/VLDL kits
Sample typeUrine, Serum, Plasma, Other biological fluids, Tissue Lysate
Assay time1h 00m
Cholesterol Assay Kit (ab65390) provides a simple quantification method for HDL and LDL/VLDL cholesterol after a convenient separation of HDL from LDL and VLDL in mammalian serum samples.
In this cholesterol assay, cholesterol oxidase specifically acts on free cholesterol to produce a chemical which reacts with a probe to generate color (570 nm) and fluorescence (Ex/Em = 538/587 nm). Cholesterol esterase is also used to hydrolyze cholesteryl ester into free cholesterol. Therefore, cholesterol ester and free cholesterol can be detected separately in the presence and absence of cholesterol esterase in the assay.
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Review our Metabolism Assay Guide to learn about assays for metabolites, metabolic enzymes, mitochondrial function, and oxidative stress, and also about how to assay metabolic function in live cells using your plate reader.
Regulation of HDL (high-density-lipoprotein)-cholesterol and LDL (low-density-lipoprotein)-cholesterol plays a central role in various disease developments. It is well known that low levels of HDL and high level of LDL are associated with an increased risk of cardiovascular events.
If you need to purchase additional HDL precipitation buffer, please see ab105138.
Storage instructionsStore at -20°C. Please refer to protocols.
Components Identifier 100 tests 2X LDL/VLDL Precipitation Buffer NW 1 x 10ml Cholesterol Assay Buffer WM, 1 x 25ml Cholesterol Esterase (Lyophilized) Blue 1 vial Cholesterol Probe (in DMSO, anhydrous) Red 1 x 200µl Cholesterol Standard (2 ug/ul) Yellow 1 x 100µl Enzyme Mix (Lyophilized) Green 1 vial
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Cholesterol Metabolism
RelevanceHDL (high-density-lipoprotein), LDL (low-density lipoprotein) and VLDL (very low-density lipoprotein) are lipoproteins that act as carrier proteins for cholesterol. The regulation of HDL-cholesterol and LDL-cholesterol plays a central role in various disease development. It is well known that low levels of HDL and high level of LDL are associated with an increased risk of cardiovascular events.
- High density lipoprotein
- Low density lipoprotein
Wild type (WT) or farnesoid X receptor knock-out (FRX-KO) mice were treated with either vehicle (Veh) or a high fat-containing diet (HFD) for 16 weeks. Serum HDL (top) and LDL/VLDL cholesterol (bottom) from 5-7 mice/group was measured using ab65390 following protocol instructions. An asterisk (*) means P<0.05 between WT and FXR-KO vehicle group. A pound (#) indicates P<0.05 between Wt and FXR-KO-HFD group. Image obtained from Li G et al., PLoS One, 2012; 7(4): e35895
Rat serum samples were processed according to the protocol. The quantity of total (dilution range 1:10-1:100), HDL (dilution range 1:1-1:10), LDL/VLDL (dilution range 1:1-1:10) and free cholesterol (neat) was measure colourimetrically, in duplicates, after 40 minutes.
Signal from standard curve was measured colourimetrically over a period of time. Background signal subtracted and each point on the curve represents duplicate values (+/- SD).
Measurement of total cholesterol, HDL, LDL/VLDL from serum samples. Total Cholesterol (blue), HDL (green), and LDL/VLDL (cream) cholesterol were measured following the kit protocol.
This product has been referenced in:
- Chae Y et al. Trophic transfer and individual impact of nano-sized polystyrene in a four-species freshwater food chain. Sci Rep 8:284 (2018). Read more (PubMed: 29321604) »
- Fernandes GW et al. The Foxo1-Inducible Transcriptional Repressor Zfp125 Causes Hepatic Steatosis and Hypercholesterolemia. Cell Rep 22:523-534 (2018). Read more (PubMed: 29320745) »