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Cardiovascular Lipids / Lipoproteins Lipid Metabolism Cholesterol Metabolism
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Cholesterol Efflux Assay Kit (Cell-based) (ab196985)

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  • Protocol Booklet
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Percentage Cholesterol Efflux

    Key features and details

    • Assay type: Cell-based (quantitative)
    • Detection method: Fluorescent
    • Platform: Microplate reader
    • Sample type: Adherent cells, Purified protein

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    Overview

    • Product name

      Cholesterol Efflux Assay Kit (Cell-based)
    • Detection method

      Fluorescent
    • Sample type

      Adherent cells, Purified protein
    • Assay type

      Cell-based (quantitative)
    • Species reactivity

      Reacts with: Mammals, Other species
    • Product overview

      Cholesterol Efflux Assay Kit ab196985 is a high-throughput, cell-based screening assay for measuring cholesterol efflux. The cholesterol efflux assay protocol uses fluorescently-labeled cholesterol (Ex/Em 482/515 nm). It provides a safe, sensitive and reproducible method for measuring cholesterol efflux.


      This cholesterol efflux assay can be used to:
      - screen small molecules for their effect on cholesterol efflux as a part of drug discovery program.


      Cholesterol efflux assay protocol summary:
      - label cells with labeling and equilibration mix overnight
      - wash cells, treat as required and incubate
      - transfer cell supernatant to microplate, and separately solubilize cells with cell lysis buffer
      - analyze supernatant and cell lysates with microplate reader

    • Notes

      Cholesterol efflux from the peripheral tissues and cells in atherosclerotic plaque is an initial and critical step in Reverse Cholesterol Transport (RCT). RCT is the process by which extra hepatic cells, including macrophage-derived foam cells in arterial atherosclerotic plaque, transport excessive cholesterol back to the liver for bile acid synthesis and excretion, thus lowering the peripheral lipid burden.

      Other cholesterol assay kits include:
      - HDL and LDL/VLDL Cholesterol assay kit ab65390 
      - Cell-based Cholesterol assay kit ab133116 
      - Cholesterol/Cholesterol Ester assay kit ab65359 
      - Cholesterol Uptake assay kit ab236212

      Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
      It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

    • Platform

      Microplate reader

    Properties

    • Storage instructions

      Store at -20°C. Please refer to protocols.
    • Components 100 tests
      Cell Lysis Buffer 1 x 20ml
      Equilibration Buffer 1 x 5ml
      Labeling Reagent 1 x 5ml
      Positive Control 1 x 1ml
      Reagent A 1 x 10µl
      Reagent B 1 x 50µl
      Serum Treatment Reagent 1 x 1ml
    • Research areas

      • Cardiovascular
      • Lipids / Lipoproteins
      • Lipid Metabolism
      • Cholesterol Metabolism
      • Kits/ Lysates/ Other
      • Kits
      • Cell Metabolism Kits
      • Lipid Metabolism Kits
      • Cholesterol
      • Kits/ Lysates/ Other
      • Kits
      • Cell Metabolism Kits
      • Lipid Metabolism Kits
      • Lipid metabolism
      • Metabolism
      • Pathways and Processes
      • Metabolic signaling pathways
      • Lipid and lipoprotein metabolism
      • Cholesterol Metabolism

    Associated products

      Images

      • Percentage Cholesterol Efflux
        Percentage Cholesterol Efflux

        J774.1 cells were labeled with the Labeling Media and treated with various cholesterol acceptors like Human Serum, HDL (50 µg) or Positive control known to cause cholesterol efflux. Cholesterol efflux is expressed as % efflux elicited by cells in 4 hours.

      Protocols

      • Protocol Booklet

      Click here to view the general protocols

      Datasheets and documents

      • SDS download

      • Datasheet download

        Download

      References (5)

      Publishing research using ab196985? Please let us know so that we can cite the reference in this datasheet.

      ab196985 has been referenced in 5 publications.

      • Shih CM  et al. Dysfunctional high density lipoprotein failed to rescue the function of oxidized low density lipoprotein-treated endothelial progenitor cells: a novel index for the prediction of HDL functionality. Transl Res 205:17-32 (2019). PubMed: 30720435
      • Wang Z  et al. MiR-30e and miR-92a are related to atherosclerosis by targeting ABCA1. Mol Med Rep 19:3298-3304 (2019). PubMed: 30816508
      • Huang Z  et al. G protein pathway suppressor 2 (GPS2) links inflammation and cholesterol efflux by controlling lipopolysaccharide-induced ATP-binding cassette transporter A1 expression in macrophages. FASEB J 33:1631-1643 (2019). PubMed: 30153049
      • Tumurkhuu G  et al. Chlamydia pneumoniae Hijacks a Host Autoregulatory IL-1ß Loop to Drive Foam Cell Formation and Accelerate Atherosclerosis. Cell Metab 28:432-448.e4 (2018). PubMed: 29937375
      • Nissilä E  et al. Complement Factor H and Apolipoprotein E Participate in Regulation of Inflammation in THP-1 Macrophages. Front Immunol 9:2701 (2018). PubMed: 30519244

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