Cholesterol Efflux Assay Kit (Cell-based) (ab196985)
- Datasheet
- References (5)
- Protocols
Overview
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Product name
Cholesterol Efflux Assay Kit (Cell-based) -
Detection method
Fluorescent -
Sample type
Adherent cells, Purified protein -
Assay type
Cell-based (quantitative) -
Species reactivity
Reacts with: Mammals, Other species -
Product overview
Cholesterol Efflux Assay Kit ab196985 is a high-throughput, cell-based screening assay for measuring cholesterol efflux. The cholesterol efflux assay protocol uses fluorescently-labeled cholesterol (Ex/Em 482/515 nm). It provides a safe, sensitive and reproducible method for measuring cholesterol efflux.
This cholesterol efflux assay can be used to:
- screen small molecules for their effect on cholesterol efflux as a part of drug discovery program.
Cholesterol efflux assay protocol summary:
- label cells with labeling and equilibration mix overnight
- wash cells, treat as required and incubate
- transfer cell supernatant to microplate, and separately solubilize cells with cell lysis buffer
- analyze supernatant and cell lysates with microplate reader -
Notes
Cholesterol efflux from the peripheral tissues and cells in atherosclerotic plaque is an initial and critical step in Reverse Cholesterol Transport (RCT). RCT is the process by which extra hepatic cells, including macrophage-derived foam cells in arterial atherosclerotic plaque, transport excessive cholesterol back to the liver for bile acid synthesis and excretion, thus lowering the peripheral lipid burden.
Other cholesterol assay kits include:
- HDL and LDL/VLDL Cholesterol assay kit ab65390
- Cell-based Cholesterol assay kit ab133116
- Cholesterol/Cholesterol Ester assay kit ab65359
- Cholesterol Uptake assay kit ab236212 -
Platform
Microplate reader
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 100 tests Cell Lysis Buffer 1 x 20ml Equilibration Buffer 1 x 5ml Labeling Reagent 1 x 5ml Positive Control 1 x 1ml Reagent A 1 x 10µl Reagent B 1 x 10µl Serum Treatment Reagent 1 x 1ml -
Research areas
Images
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Percentage Cholesterol EffluxJ774.1 cells were labeled with the Labeling Media and treated with various cholesterol acceptors like Human Serum, HDL (50 µg) or Positive control known to cause cholesterol efflux. Cholesterol efflux is expressed as % efflux elicited by cells in 4 hours.
Protocols
References
This product has been referenced in:
- Shih CM et al. Dysfunctional high density lipoprotein failed to rescue the function of oxidized low density lipoprotein-treated endothelial progenitor cells: a novel index for the prediction of HDL functionality. Transl Res 205:17-32 (2019). Read more (PubMed: 30720435) »
- Wang Z et al. MiR-30e and miR-92a are related to atherosclerosis by targeting ABCA1. Mol Med Rep 19:3298-3304 (2019). Read more (PubMed: 30816508) »
