Overview

  • Product name
    Cholesterol Efflux Assay Kit (Cell-based)
  • Detection method
    Fluorescent
  • Sample type
    Adherent cells, Purified protein
  • Assay type
    Cell-based (quantitative)
  • Species reactivity
    Reacts with: Mammals, Other species
  • Product overview

    Cholesterol Efflux Assay Kit ab196985 is a high-throughput, cell-based screening assay for measuring cholesterol efflux. The cholesterol efflux assay protocol uses fluorescently-labeled cholesterol (Ex/Em 482/515 nm). It provides a safe, sensitive and reproducible method for measuring cholesterol efflux.


    This cholesterol efflux assay can be used to:
    - screen small molecules for their effect on cholesterol efflux as a part of drug discovery program.


    Cholesterol efflux assay protocol summary:
    - label cells with labeling and equilibration mix overnight
    - wash cells, treat as required and incubate
    - transfer cell supernatant to microplate, and separately solubilize cells with cell lysis buffer
    - analyze supernatant and cell lysates with microplate reader

  • Notes

    Cholesterol efflux from the peripheral tissues and cells in atherosclerotic plaque is an initial and critical step in Reverse Cholesterol Transport (RCT). RCT is the process by which extra hepatic cells, including macrophage-derived foam cells in arterial atherosclerotic plaque, transport excessive cholesterol back to the liver for bile acid synthesis and excretion, thus lowering the peripheral lipid burden.

    Other cholesterol assay kits include:
    HDL and LDL/VLDL Cholesterol assay kit ab65390 
    - Cell-based Cholesterol assay kit ab133116 
    Cholesterol/Cholesterol Ester assay kit ab65359 
    Cholesterol Uptake assay kit ab236212

  • Platform
    Microplate reader

Properties

Images

  • J774.1 cells were labeled with the Labeling Media and treated with various cholesterol acceptors like Human Serum, HDL (50 µg) or Positive control known to cause cholesterol efflux. Cholesterol efflux is expressed as % efflux elicited by cells in 4 hours.

Protocols

References

This product has been referenced in:
  • Shih CM  et al. Dysfunctional high density lipoprotein failed to rescue the function of oxidized low density lipoprotein-treated endothelial progenitor cells: a novel index for the prediction of HDL functionality. Transl Res 205:17-32 (2019). Read more (PubMed: 30720435) »
  • Tumurkhuu G  et al. Chlamydia pneumoniae Hijacks a Host Autoregulatory IL-1ß Loop to Drive Foam Cell Formation and Accelerate Atherosclerosis. Cell Metab 28:432-448.e4 (2018). Read more (PubMed: 29937375) »
See all 3 Publications for this product

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