The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Dot: Use at an assay dependent dilution.
ELISA: 1/4000 - 1/20000.
This product has been assayed against 1.0µg of Cholesterol Oxidase in a standard capture ELISA using Peroxidase conjugated streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic-acid]) as a substrate for 30 minutes at RT.
IM: Use at an assay dependent dilution.
WB: Use at an assay dependent dilution. Predicted molecular weight: 57 kDa.
Suitable for other antibody based assays using streptavidin or avidin conjugates.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Cholesterol Oxidases exist as both type I and type II oxidases and are implicated in bacterial pathogenesis. In addition, they are important as clinical reagents, potential larvicides, and tools in cell biology.