Overview

  • Product name

    Cholesterol Uptake Assay Kit
  • Detection method

    Fluorescent
  • Sample type

    Adherent cells, Suspension cells
  • Product overview

    Cholesterol Uptake Assay Kit (ab236212) provides a convenient tool for studying cellulat cholesterol trafficking. The kit employs NBD Cholesterol, a fluorescently-tagged cholesterol, as a probe for the detection of cholesterol taken up by cultured cells. U-18666A, which increases cholesterol uptake by inhibiting trafficking of synthesized cholesterol, is included as a positive control. The kit provides enough NBD Cholesterol to test 250 samples in a 96-well format.

  • Notes

  • Platform

    Microplate reader, Fluor. microscope, Flow cyt.

Properties

  • Storage instructions

    Please refer to protocols.
  • Components 1 kit
    Cell-Based Assay Buffer Tablet 1 tablet
    Cell-Based Assay NBD Cholesterol 1 x 500µl
    Cell-Based Assay U-18666A 1 x 100µl

Images

  • Jurkat cells were seeded at a density of 5x105 cells/mL and incubated overnight in serum-free RPMI with U-18666A or vehicle and 20 µg/mL NBD Cholesterol in a cell culture incubator at 37°C. The next day, cells were transferred to a v-bottom plate for washing and reading on a flow cytometer. Cholesterol uptake was evaluated in the live cell gate using FlowJo analysis software. U-18666A at both 2.5 µM (green) and 1.25 µM (blue) showed a significant (p<0.05, t-test) shift in mean fluorescence as compared to the vehicle control (black) and the untreated (red) cells.

Protocols

References

ab236212 has not yet been referenced specifically in any publications.

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