Key features and details
- Assay type: Quantitative
- Detection method: Colorimetric
- Platform: Microplate reader
- Assay time: 1 hr
- Sample type: Cell Lysate, Tissue Lysate
Product nameCholesterol/Cholesteryl Ester Assay Kit - Detection
See all Cholesterol/Cholesteryl Ester kits
Sample typeCell Lysate, Tissue Lysate
Assay time1h 00m
Cholesterol/Cholesteryl Ester Assay Kit ab102515 uses a simple method for sensitive quantification of free cholesterol, cholesteryl esters, or both using a colorimetric method.
This assay kit uses an alternative method compared to our most popular Cholesterol/ Cholesteryl Ester Assay Kit ab65359 (which relies on a cholesterol oxidase based method).
In this assay kit, free cholesterol is oxidized by cholesterol dehydrogenase to generate NADH which reacts with a sensitive probe resulting in strong absorbance at 450 nm.
The assay can detect free or total cholesterol depending upon whether cholesterol esterase is used to hydrolyze cholesterol esters present.
Cholesteryl ester can be determined by subtracting the value of free cholesterol from the total cholesterol (cholesterol plus cholesteryl esters).
This product is manufactured by BioVision, an Abcam company and was previously called K623 Total Cholesterol and Cholesteryl Ester Colorimetric Assay Kit II. K623-100 is the same size as the 100 test size of ab102515.
Cholesterol is an essential molecule in all animal life. It has been involved in both normal development and diseases.
Storage instructionsStore at -20°C. Please refer to protocols.
Components Identifier 100 tests Cholesterol Assay Buffer WM 1 x 25ml Cholesterol Standard Yellow 1 x 100µl Enzyme Mix (lyophilized) Green 1 vial Esterase (lyophilized) Blue 1 vial Substrate Mix (lyophilized) Red 1 vial
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Cholesterol Metabolism
RelevanceCholesterol is an essential molecule in all animal life. It is involved in both normal development and diseases. A high level of serum cholesterol is an indicator for diseases such as heart disease. Cholesteryl ester is an ester of cholesterol. The ester bond is formed between the carboxylate group of a fatty acid and the hydroxyl group of cholesterol.
ab102515 has been referenced in 3 publications.
- Ali A et al. CAV1 - GLUT3 signaling is important for cellular energy and can be targeted by Atorvastatin in Non-Small Cell Lung Cancer. Theranostics 9:6157-6174 (2019). PubMed: 31534543
- Liang X et al. Organic cation transporter 1 (OCT1) modulates multiple cardiometabolic traits through effects on hepatic thiamine content. PLoS Biol 16:e2002907 (2018). PubMed: 29659562
- Li X et al. Fatostatin displays high antitumor activity in prostate cancer by blocking SREBP-regulated metabolic pathways and androgen receptor signaling. Mol Cancer Ther 13:855-66 (2014). PubMed: 24493696