Overview

  • Product name
    Anti-Choline Acetyltransferase antibody
    See all Choline Acetyltransferase primary antibodies
  • Description
    Sheep polyclonal to Choline Acetyltransferase
  • Host species
    Sheep
  • Tested applications
    Suitable for: IHC-FoFr, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Rabbit, Guinea pig, Human, Pig
    Predicted to work with: Chicken
  • Immunogen

    Synthetic peptide:

    GLFSSYRLPGHTQDTLVAQKSS

    conjugated to KLH, corresponding to amino acids 167-188 of Pig Choline Acetyltransferase.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Add glycerol to a final volume of 50% for extra stability and aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Constituent: Whole serum
  • Concentration information loading...
  • Purity
    Ammonium Sulphate Precipitation
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab18736 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-FoFr 1/1000.

Use on perfusion fixed tissue (1% sodium Nitrite in 0.1M TBS, pH, 7.4 for 20 min then 4% formaldehyde containing 15% picric acid in 0.1M TBS,pH, 7.4 for 20min). Please note sections must be post fixed if the tissue is cut freshly.

IHC-P Use at an assay dependent concentration.
ICC/IF 1/100. PubMed: 18393628

Target

Images

  • ab18736 staining Choline Acetyltransferase in mouse brain tissue sections by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue samples were fixed by perfusion with paraformaldehyde, permeablized with 0.3% Triton X-100 and blocked with 3% donkey serum in 0.3% Triton X-100/TBS for 1 hour at 21°C. The sample was incubated with primary antibody (1/1000 in 1% donkey serum + 0.3% Triton X-100/TBS) at 4°C for 24 hours. An Alexa Fluor® 594-conjugated donkey anti-sheep polyclonal (1/200) was used as the secondary antibody.

    See Abreview

  • Immunofluorescence of Rat Forebrain tissue labeling ChAT with ab18736 using a 1µg dilution. 

     

  • Immunohistochemical detection of ChAT in rat basal forebrain using Sheep anti ChAT (ab18736).

    Rat was perfused with 1% sodium Nitrite in 0.1M TBS, pH, 7.4 for 20 min. and then fixed with 4% formaldehyde containing 15% picric acid in 0.1M TBS, pH, 7.4 for 30 min. The brain was then cryoprotected in 30% overnight and cut into 40 nm free-floating sections. Sections were washed 2x with TBS for 20 min. and passed through asending ethanol and finally transferred to 0.1 M TBS for rehydration for 1h.

References

This product has been referenced in:
  • Wang L  et al. Motor neuron degeneration following glycine-mediated excitotoxicity induces spastic paralysis after spinal cord ischemia/reperfusion injury in rabbit. Am J Transl Res 9:3411-3421 (2017). Read more (PubMed: 28804557) »
  • Yu B  et al. Mechanisms Underlying Footshock and Psychological Stress-Induced Abrupt Awakening From Posttraumatic "Nightmares". Int J Neuropsychopharmacol N/A:N/A (2015). Rat . Read more (PubMed: 26591007) »
See all 10 Publications for this product

Customer reviews and Q&As

1-6 of 6 Abreviews or Q&A

Application
IHC - Wholemount
Sample
Rat Tissue (Myenteric plexus from rat colon)
Specification
Myenteric plexus from rat colon

Abcam user community

Verified customer

Submitted Aug 23 2016

Application
IHC - Wholemount
Sample
Mouse Tissue (myenteric plexus from colon)
Specification
myenteric plexus from colon

Abcam user community

Verified customer

Submitted Aug 22 2016

Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Blocking step
Donkey Serum in 0.3% Triton X-100/TBS as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C
Antigen retrieval step
None
Sample
Mouse Tissue sections (Brain)
Specification
Brain
Permeabilization
Yes - 0.3% Triton X-100
Fixative
Paraformaldehyde

Ms. Carla Cabral

Verified customer

Submitted Mar 28 2014

Answer

Thank you very much for your interest in our products. The following antibodies are tested and guaranteed for IHC-P in rat. Unfortunately, we do not have blocking peptide for these antibodies in our catalogue. ab68779 Click here (or use the following: https://www.abcam.com/index.html?datasheet=68779). ab6168 Click here (or use the following: https://www.abcam.com/index.html?datasheet=6168). ab70219 Click here (or use the following: https://www.abcam.com/index.html?datasheet =70219). ab18736 Click here (or use the following: https://www.abcam.com/index.html?datasheet=18736). ab34419 Click here (or use the following: https://www.abcam.com/index.html?datasheet=34419). The following antibodies have a blocking peptide readily available, but are not tested and guaranteed for IHC-P. Therefore you might be eligible for our testing discount program if you would like to test one of these antibodies in IHC-P on rat samples. ab85609 with ab97420 Click here (or use the following: https://www.abcam.com/index.html?datasheet=85609). ab50412 with ab50440 Click here (or use the following: https://www.abcam.com/index.html?datasheet=50412). ab27484 with ab45678 Click here (or use the following: https://www.abcam.com/index.html?datasheet=27484). Please do not hesitate to contact me again with any further questions or if you consider testing one of the latter antibodies in IHC-P.

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (brain CA1)
Specification
brain CA1
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: EDTA
Permeabilization
Yes - 0.1% IGEPAL CA630
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C

Abcam user community

Verified customer

Submitted Apr 10 2009

Answer

Thank you for your enquiry. Please find below the answers to your questions: Q1. was the fix of 4% formaldehyde containing 15% picric acid in 0.1M TBS,pH, 7.4 for 30 min done after the brain was extracted, and if so was the whole brain simply immersed in this solution for 30 mins or was there a perfusion step? If the brain was immersed - how do we know the fixative penetrated the entire brain in 30 mins? A. Animal is perfused with the fixative, then the brain is extracted and sectioned. Fresh brains can not be fixed properly in only 30 min. Q2. We find that we need to fix in 4% formaldehyde perfusion to extract spinal cord, what would be the effect of the perfusion? A. Perfusion with 4% PFA should not adversely affect the antigenicity of ChAT. We do that routinely without any problem. Q2Was staining done subsequently to rehydration? A. Please see the attached protocol on the datasheet. Q3. What were the ascending ethanol steps? A. Please see the attached protocol on the datasheet. Q4. Could this protocol be used on mounted sections (we have to mount spinal cord before fixation). If we do, should we apply the ethanol to frozen sections/slides, or should we let the slides reach room temp before starting to fix?? A. Please see the attached protocol for free floating section. Just note that you have to post-fix your sections if you are cutting your tissue freshly. Please do not hesitate to contact us if you require further assistance,

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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