Overview

  • Product name

    Anti-Choline Acetyltransferase antibody
    See all Choline Acetyltransferase primary antibodies
  • Description

    Rabbit polyclonal to Choline Acetyltransferase
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide corresponding to Human Choline Acetyltransferase. (From the N-terminal to the mid-protein).
    Database link: P28329

  • Positive control

    • WB: Mouse brain lysate. ICC/IF: SK-N-BE cells.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: 50% Glycerol, PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab223346 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 83 kDa.
ICC/IF 1/100.

Target

  • Function

    Catalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses.
  • Involvement in disease

    Myasthenic syndrome, congenital, 6, presynaptic
  • Sequence similarities

    Belongs to the carnitine/choline acetyltransferase family.
  • Information by UniProt
  • Database links

  • Alternative names

    • Acetyl CoA choline O acetyltransferase antibody
    • Acetyl CoA:choline O acetyltransferase antibody
    • ChAT antibody
    • CHOACTase antibody
    • Choline acetylase antibody
    • choline acetyltransferase antibody
    • Choline O acetyltransferase antibody
    • Choline O-acetyltransferase antibody
    • CLAT_HUMAN antibody
    • CMS1A antibody
    • CMS1A2 antibody
    • EC 2.3.1.6 antibody
    • OTTHUMP00000019583 antibody
    • OTTHUMP00000019584 antibody
    see all

Images

  • 4% formaldehyde-fixed SK-N-BE cells stained for Choline Acetyltransferase (green) using ab223346 at 1/100 dilution in ICC/IF. Secondary: Goat Anti-Rabbit ATTO 488 at 1/100 for 60 minutes at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1/1000, 1/5000 for 60 minutes RT, 5 minutes RT.

    (A) DAPI nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) ab223346 (D) Merge.

  • Anti-Choline Acetyltransferase antibody (ab223346) at 1/1000 dilution + Mouse brain lysate at 20 µg

    Secondary
    Goat Anti-Rabbit: HRP at 1/2000 dilution

    Predicted band size: 83 kDa



    Blocking: 5% milk + TBST for 1 hour at RT.

    Color Development: TMB solution for 12 minutes at RT.

References

ab223346 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab223346.
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