Key features and details
- Rabbit polyclonal to Choline Acetyltransferase
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-Choline Acetyltransferase antibody
See all Choline Acetyltransferase primary antibodies
DescriptionRabbit polyclonal to Choline Acetyltransferase
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Mouse, Human
Synthetic peptide corresponding to Human Choline Acetyltransferase. (From the N-terminal to the mid-protein).
Database link: P28329
- WB: Mouse brain lysate. ICC/IF: SK-N-BE cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab223346 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Predicted molecular weight: 83 kDa.|
FunctionCatalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses.
Involvement in diseaseMyasthenic syndrome, congenital, 6, presynaptic
Sequence similaritiesBelongs to the carnitine/choline acetyltransferase family.
- Information by UniProt
- Acetyl CoA choline O acetyltransferase antibody
- Acetyl CoA:choline O acetyltransferase antibody
- ChAT antibody
4% formaldehyde-fixed SK-N-BE cells stained for Choline Acetyltransferase (green) using ab223346 at 1/100 dilution in ICC/IF. Secondary: Goat Anti-Rabbit ATTO 488 at 1/100 for 60 minutes at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1/1000, 1/5000 for 60 minutes RT, 5 minutes RT.
(A) DAPI nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) ab223346 (D) Merge.
Anti-Choline Acetyltransferase antibody (ab223346) at 1/1000 dilution + Mouse brain lysate at 20 µg
Goat Anti-Rabbit: HRP at 1/2000 dilution
Predicted band size: 83 kDa
Blocking: 5% milk + TBST for 1 hour at RT.
Color Development: TMB solution for 12 minutes at RT.
ab223346 has not yet been referenced specifically in any publications.