Overview

  • Product name
    Anti-Choline Acetyltransferase antibody
    See all Choline Acetyltransferase primary antibodies
  • Description
    Chicken polyclonal to Choline Acetyltransferase
  • Host species
    Chicken
  • Tested applications
    Suitable for: WB, ICC/IF, IHC-Fr, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (Mouse)

  • Positive control
    • Mouse/rat brain.

Properties

Applications

Our Abpromise guarantee covers the use of ab34419 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/250 - 1/500. Predicted molecular weight: 83 kDa.
ICC/IF 1/100 - 1/200.
IHC-Fr 1/50.
IHC-P Use at an assay dependent dilution.

Target

Images

  • This image shows a section of a formalin-fixed paraffin-embedded adult mouse brain showing ChAT (red staining) in cholinergic neurons of the caudate-putamen nucleus. Antibody ab34419 at a dilution of 1/100.

References

This product has been referenced in:
  • Bricker-Anthony C  et al. Molecular changes and vision loss in a mouse model of closed-globe blast trauma. Invest Ophthalmol Vis Sci 55:4853-62 (2014). Mouse . Read more (PubMed: 24994864) »
See 1 Publication for this product

Customer reviews and Q&As

1-5 of 5 Abreviews or Q&A

Application
Immunohistochemistry free floating
Sample
Rat Tissue sections (Brain striatum)
Specification
Brain striatum

Dr. Alexandre Magno

Verified customer

Submitted Aug 15 2018

Answer

Thank you for contacting Abcam. I am sorry to hear that you are still not able to get any convincing staining using ab34419. I would be happy to get you a replacement, the ab70219, I am having a little difficulty finding your original order though, so if you could send me either the PO# or order number that was used, that would be great. I look forward to your reply and getting you a replacement antibody.

Read More

Answer

Thank you for updating me on the other protocol methods that you have tried. Please let me know how diluting the antibody in PBS rather than blocking buffer goes. I look forward to hearing from you and good luck with the staining toda

Read More

Question

LOT NUMBER 811618 ORDER NUMBER -- NOT SPECIFIED -- DESCRIPTION OF THE PROBLEM No staining SAMPLE Mouse colon PRIMARY ANTIBODY Chicken polyclonal to Choline Acetyltransferase (ab34419), dilution 1:100 in blocking solution, incubation overnight at 4 oC, washes 3x 10 min with PBST (0,1% Triton) DETECTION METHOD Fluorescence microscopy POSITIVE AND NEGATIVE CONTROLS USED positive control: mouse brain negative control: no primary antibody In both cases no staining was observed ANTIBODY STORAGE CONDITIONS Stored at 4 oC in the dark as indicated by the datasheet. FIXATION OF SAMPLE 4% PFA for 1h ANTIGEN RETRIEVAL Tested both with antigen retrieval (10 mM Sodium Citrate Buffer, pH 6.0 for 2x 5 minutes at 95°C) and without antigen retrieval. PERMEABILIZATION STEP no BLOCKING CONDITIONS 10% Normal Goat Serum for 30 min at RT. SECONDARY ANTIBODY FITC-AffiniPure Dnk Anti-Chicken IgY (IgG) (H+L) (703-095-155), dilution 1:500 in blocking solution for 2h at RT HOW MANY TIMES HAVE YOU TRIED THE APPLICATION? 3-4 HAVE YOU RUN A "NO PRIMARY" CONTROL? Yes DO YOU OBTAIN THE SAME RESULTS EVERY TIME? Yes WHAT STEPS HAVE YOU ALTERED? antigen retrieval step (with or without) blocking step (with or without) permeabilization step (20 min PBS with 0.5% TritonX) ADDITIONAL NOTES We have purchased this antibody some months ago but due to problems in the lab, i didn't have the chance to test it until recently. I understand that because of that i cannot ask for a refund or a replacement but i would greatly apprecia

Read More
Answer

Thank you for contacting Abcam about ab34419, Chicken polyclonal to Choline Acetyltransferase. I am sorry to hear that you are not able to get any staining using the antibody. I have looked over your methods that you provided and it seems that you have done an extensive amount of testing to try and get this antibody to work. A few things I thought of that may be of help are: 1- Have you successfully used this secondary anti body before? 2-You dilute the primary antibody in blocking buffer, did you try diluting in PBS instead of the blocking buffer? 3- The fixation time of 1 hour may be to long, reducing it to 45 minutes may help. The antibody ab34419, is covered under our Abpromise, which is that we guarantee that it will work in the species and applications as stated on our website. As this antibody is not performing as stated and if the above tips prove not to be helpful then I will be happy to provide you with a free of charge replacement or a refund. I did to a search for other anti-Choline Acetyltransferase and there are a few that I found that may be a suitable replacement: ab68779 ab70219 ab18736 Please let me know if you have already tried the above protocol tips or if you would like to take advantage of our Abpromise and I will be more than happy to get that sorted out for you. I hope you ha

Read More

Answer

Thank you very much for your interest in our products. The following antibodies are tested and guaranteed for IHC-P in rat. Unfortunately, we do not have blocking peptide for these antibodies in our catalogue. ab68779 Click here (or use the following: https://www.abcam.com/index.html?datasheet=68779). ab6168 Click here (or use the following: https://www.abcam.com/index.html?datasheet=6168). ab70219 Click here (or use the following: https://www.abcam.com/index.html?datasheet =70219). ab18736 Click here (or use the following: https://www.abcam.com/index.html?datasheet=18736). ab34419 Click here (or use the following: https://www.abcam.com/index.html?datasheet=34419). The following antibodies have a blocking peptide readily available, but are not tested and guaranteed for IHC-P. Therefore you might be eligible for our testing discount program if you would like to test one of these antibodies in IHC-P on rat samples. ab85609 with ab97420 Click here (or use the following: https://www.abcam.com/index.html?datasheet=85609). ab50412 with ab50440 Click here (or use the following: https://www.abcam.com/index.html?datasheet=50412). ab27484 with ab45678 Click here (or use the following: https://www.abcam.com/index.html?datasheet=27484). Please do not hesitate to contact me again with any further questions or if you consider testing one of the latter antibodies in IHC-P.

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up