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    choline-acetyltransferase-antibody-ab34419.pdf

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Neuroscience Neurotransmitter Acetylcholine
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Anti-Choline Acetyltransferase antibody (ab34419)

  • Datasheet
Reviews (1)Q&A (4)References (2)

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Immunocytochemistry/ Immunofluorescence - Anti-Choline Acetyltransferase antibody (ab34419)

    Key features and details

    • Chicken polyclonal to Choline Acetyltransferase
    • Suitable for: ICC/IF
    • Reacts with: Mouse
    • Isotype: IgY

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    Overview

    • Product name

      Anti-Choline Acetyltransferase antibody
      See all Choline Acetyltransferase primary antibodies
    • Description

      Chicken polyclonal to Choline Acetyltransferase
    • Host species

      Chicken
    • Tested Applications & Species

      Application Species
      ICC/IF
      Mouse
      See all applications and species data
    • Immunogen

      Synthetic peptide (Mouse)

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
    • Storage buffer

      Preservative: 0.02% Sodium azide
      Constituent: PBS
    • Concentration information loading...
    • Purity

      Protein L purified
    • Clonality

      Polyclonal
    • Isotype

      IgY
    • Research areas

      • Neuroscience
      • Neurotransmitter
      • Acetylcholine
      • Neuroscience
      • Cell Type Marker
      • Neuron marker
      • Soma marker

    Associated products

    • Compatible Secondaries

      • Rabbit Anti-Chicken IgY H&L (FITC) (ab6749)
      • Rabbit Anti-Chicken IgY H&L (Biotin) (ab6752)
      • Rabbit Anti-Chicken IgY H&L (HRP) (ab6753)
      • Goat Anti-Chicken IgY H&L (HRP) (ab6877)
      • Goat Anti-Chicken IgY H&L (Biotin) preadsorbed (ab7117)
    • Isotype control

      • Chicken IgY - Isotype Control (ab50579)
    • Recombinant Protein

      • Recombinant Rat Choline Acetyltransferase protein (ab119467)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab34419 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Guaranteed

    Tested applications are guaranteed to work and covered by our Abpromise guarantee.

    Predicted

    Predicted to work for this combination of applications and species but not guaranteed.

    Incompatible

    Does not work for this combination of applications and species.

    Application Species
    ICC/IF
    Mouse
    Application Abreviews Notes
    ICC/IF
    1/100 - 1/200.
    Notes
    ICC/IF
    1/100 - 1/200.

    Target

    • Function

      Catalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses.
    • Involvement in disease

      Myasthenic syndrome, congenital, 6, presynaptic
    • Sequence similarities

      Belongs to the carnitine/choline acetyltransferase family.
    • Target information above from: UniProt accession P28329 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 12647 Mouse
      • SwissProt: Q03059 Mouse
      • Unigene: 442817 Mouse
      • Alternative names

        • Acetyl CoA choline O acetyltransferase antibody
        • Acetyl CoA:choline O acetyltransferase antibody
        • ChAT antibody
        • CHOACTase antibody
        • Choline acetylase antibody
        • choline acetyltransferase antibody
        • Choline O acetyltransferase antibody
        • Choline O-acetyltransferase antibody
        • CLAT_HUMAN antibody
        • CMS1A antibody
        • CMS1A2 antibody
        • EC 2.3.1.6 antibody
        • OTTHUMP00000019583 antibody
        • OTTHUMP00000019584 antibody
        see all

      Images

      • Immunocytochemistry/ Immunofluorescence - Anti-Choline Acetyltransferase antibody (ab34419)
        Immunocytochemistry/ Immunofluorescence - Anti-Choline Acetyltransferase antibody (ab34419)
        This image shows a section of a formalin-fixed paraffin-embedded adult mouse brain showing ChAT (red staining) in cholinergic neurons of the caudate-putamen nucleus. Antibody ab34419 at a dilution of 1/100.

      Protocols

      • Immunocytochemistry & immunofluorescence protocols

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
    • References (2)

      Publishing research using ab34419? Please let us know so that we can cite the reference in this datasheet.

      ab34419 has been referenced in 2 publications.

      • Bricker-Anthony C  et al. Molecular changes and vision loss in a mouse model of closed-globe blast trauma. Invest Ophthalmol Vis Sci 55:4853-62 (2014). Mouse . PubMed: 24994864
      • Bricker-Anthony C  et al. Exacerbation of blast-induced ocular trauma by an immune response. J Neuroinflammation 11:192 (2014). PubMed: 25472427

      Customer reviews and Q&As

      Show All Reviews Q&A
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      1-5 of 5 Abreviews or Q&A

      Immunohistochemistry free floating abreview for Anti-Choline Acetyltransferase antibody

      Excellent
      Abreviews
      Abreviews
      abreview image
      Application
      Immunohistochemistry free floating
      Sample
      Rat Tissue sections (Brain striatum)
      Specification
      Brain striatum
      Read More

      Dr. Alexandre Magno

      Verified customer

      Submitted Aug 15 2018

      Question

      unfortunately i have to inform you that the ChAT antibody (ab34419) didn't work even after following your suggestion to dilute the primary antibody in PBS instead of blocking solution. In your previous e-mail you recommended a few antibodies as suitable replacements. I would be interested in the ab70219 anti-ChAT antibody. Please let me know if i should send you back the other antibody or if you need anything else for the replacement. Thank you for your cooperation

      Read More

      Abcam community

      Verified customer

      Asked on Oct 12 2011

      Answer

      Thank you for contacting Abcam. I am sorry to hear that you are still not able to get any convincing staining using ab34419. I would be happy to get you a replacement, the ab70219, I am having a little difficulty finding your original order though, so if you could send me either the PO# or order number that was used, that would be great. I look forward to your reply and getting you a replacement antibody.

      Read More

      Abcam Scientific Support

      Answered on Oct 12 2011

      Question

      Dear Dr Constable, thank you very much for your reply. Regarding your suggestions: I have used this secondary antibody before without problem and the fixation time i mentioned refers to the tissue i use for my experiments (mouse colon). In these experiments other samples were included (mouse brain as positive control) which had shorter fixation time (about 30-45 min) and again had no staining. I haven't tried diluting the antibody in PBS so i will try it today and i will inform you about the results. Thank yo

      Read More

      Abcam community

      Verified customer

      Asked on Oct 10 2011

      Answer

      Thank you for updating me on the other protocol methods that you have tried. Please let me know how diluting the antibody in PBS rather than blocking buffer goes. I look forward to hearing from you and good luck with the staining toda

      Read More

      Abcam Scientific Support

      Answered on Oct 10 2011

      Question

      LOT NUMBER 811618 ORDER NUMBER -- NOT SPECIFIED -- DESCRIPTION OF THE PROBLEM No staining SAMPLE Mouse colon PRIMARY ANTIBODY Chicken polyclonal to Choline Acetyltransferase (ab34419), dilution 1:100 in blocking solution, incubation overnight at 4 oC, washes 3x 10 min with PBST (0,1% Triton) DETECTION METHOD Fluorescence microscopy POSITIVE AND NEGATIVE CONTROLS USED positive control: mouse brain negative control: no primary antibody In both cases no staining was observed ANTIBODY STORAGE CONDITIONS Stored at 4 oC in the dark as indicated by the datasheet. FIXATION OF SAMPLE 4% PFA for 1h ANTIGEN RETRIEVAL Tested both with antigen retrieval (10 mM Sodium Citrate Buffer, pH 6.0 for 2x 5 minutes at 95°C) and without antigen retrieval. PERMEABILIZATION STEP no BLOCKING CONDITIONS 10% Normal Goat Serum for 30 min at RT. SECONDARY ANTIBODY FITC-AffiniPure Dnk Anti-Chicken IgY (IgG) (H+L) (703-095-155), dilution 1:500 in blocking solution for 2h at RT HOW MANY TIMES HAVE YOU TRIED THE APPLICATION? 3-4 HAVE YOU RUN A "NO PRIMARY" CONTROL? Yes DO YOU OBTAIN THE SAME RESULTS EVERY TIME? Yes WHAT STEPS HAVE YOU ALTERED? antigen retrieval step (with or without) blocking step (with or without) permeabilization step (20 min PBS with 0.5% TritonX) ADDITIONAL NOTES We have purchased this antibody some months ago but due to problems in the lab, i didn't have the chance to test it until recently. I understand that because of that i cannot ask for a refund or a replacement but i would greatly apprecia

      Read More

      Abcam community

      Verified customer

      Asked on Oct 07 2011

      Answer

      Thank you for contacting Abcam about ab34419, Chicken polyclonal to Choline Acetyltransferase. I am sorry to hear that you are not able to get any staining using the antibody. I have looked over your methods that you provided and it seems that you have done an extensive amount of testing to try and get this antibody to work. A few things I thought of that may be of help are: 1- Have you successfully used this secondary anti body before? 2-You dilute the primary antibody in blocking buffer, did you try diluting in PBS instead of the blocking buffer? 3- The fixation time of 1 hour may be to long, reducing it to 45 minutes may help. The antibody ab34419, is covered under our Abpromise, which is that we guarantee that it will work in the species and applications as stated on our website. As this antibody is not performing as stated and if the above tips prove not to be helpful then I will be happy to provide you with a free of charge replacement or a refund. I did to a search for other anti-Choline Acetyltransferase and there are a few that I found that may be a suitable replacement: ab68779 ab70219 ab18736 Please let me know if you have already tried the above protocol tips or if you would like to take advantage of our Abpromise and I will be more than happy to get that sorted out for you. I hope you ha

      Read More

      Abcam Scientific Support

      Answered on Oct 07 2011

      Question

      Dear Sir, Madam,   I am "an old customer" and I would like to enquire: I plan to study the protein expression of choline acetyltransferase in rat pancreas, in formalin fixed, paraffin-embedded tissue. Is there any of the various antibodies available that also is equipped with an immungenic peptide ("blocking peptide") as an additional negative control?     Yours sincerely  

      Read More

      Abcam community

      Verified customer

      Asked on Sep 12 2011

      Answer

      Thank you very much for your interest in our products. The following antibodies are tested and guaranteed for IHC-P in rat. Unfortunately, we do not have blocking peptide for these antibodies in our catalogue. ab68779 Click here (or use the following: https://www.abcam.com/Choline-Acetyltransferase-antibody-ab68779.html). ab6168 Click here (or use the following: https://www.abcam.com/Choline-Acetyltransferase-antibody-ab6168.html). ab70219 Click here (or use the following: https://www.abcam.com/index.html?datasheet =70219). ab18736 Click here (or use the following: https://www.abcam.com/Choline-Acetyltransferase-antibody-ab18736.html). ab34419 Click here (or use the following: https://www.abcam.com/Choline-Acetyltransferase-antibody-ab34419.html). The following antibodies have a blocking peptide readily available, but are not tested and guaranteed for IHC-P. Therefore you might be eligible for our testing discount program if you would like to test one of these antibodies in IHC-P on rat samples. ab85609 with ab97420 Click here (or use the following: https://www.abcam.com/Choline-Acetyltransferase-antibody-C-terminal-ab85609.html). ab50412 with ab50440 Click here (or use the following: https://www.abcam.com/Choline-Acetyltransferase-antibody-ab50412.html). ab27484 with ab45678 Click here (or use the following: https://www.abcam.com/Choline-Acetyltransferase-antibody-ab27484.html). Please do not hesitate to contact me again with any further questions or if you consider testing one of the latter antibodies in IHC-P.

      Read More

      Abcam Scientific Support

      Answered on Sep 12 2011

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