Anti-Choline Acetyltransferase antibody (ab34419)

Thank you for contacting Abcam. I am sorry to hear that you are still not able to get any convincing staining using ab34419. I would be happy to get you a replacement, the ab70219, I am having a little difficulty finding your original order though, so if you could send me either the PO# or order number that was used, that would be great. I look forward to your reply and getting you a replacement antibody.

Thank you for updating me on the other protocol methods that you have tried. Please let me know how diluting the antibody in PBS rather than blocking buffer goes. I look forward to hearing from you and good luck with the staining toda

Thank you for contacting Abcam about ab34419, Chicken polyclonal to Choline Acetyltransferase. I am sorry to hear that you are not able to get any staining using the antibody. I have looked over your methods that you provided and it seems that you have done an extensive amount of testing to try and get this antibody to work. A few things I thought of that may be of help are: 1- Have you successfully used this secondary anti body before? 2-You dilute the primary antibody in blocking buffer, did you try diluting in PBS instead of the blocking buffer? 3- The fixation time of 1 hour may be to long, reducing it to 45 minutes may help. The antibody ab34419, is covered under our Abpromise, which is that we guarantee that it will work in the species and applications as stated on our website. As this antibody is not performing as stated and if the above tips prove not to be helpful then I will be happy to provide you with a free of charge replacement or a refund. I did to a search for other anti-Choline Acetyltransferase and there are a few that I found that may be a suitable replacement: ab68779 ab70219 ab18736 Please let me know if you have already tried the above protocol tips or if you would like to take advantage of our Abpromise and I will be more than happy to get that sorted out for you. I hope you ha

Thank you very much for your interest in our products. The following antibodies are tested and guaranteed for IHC-P in rat. Unfortunately, we do not have blocking peptide for these antibodies in our catalogue. ab68779 Click here (or use the following: https://www.abcam.com/index.html?datasheet=68779). ab6168 Click here (or use the following: https://www.abcam.com/index.html?datasheet=6168). ab70219 Click here (or use the following: https://www.abcam.com/index.html?datasheet =70219). ab18736 Click here (or use the following: https://www.abcam.com/index.html?datasheet=18736). ab34419 Click here (or use the following: https://www.abcam.com/index.html?datasheet=34419). The following antibodies have a blocking peptide readily available, but are not tested and guaranteed for IHC-P. Therefore you might be eligible for our testing discount program if you would like to test one of these antibodies in IHC-P on rat samples. ab85609 with ab97420 Click here (or use the following: https://www.abcam.com/index.html?datasheet=85609). ab50412 with ab50440 Click here (or use the following: https://www.abcam.com/index.html?datasheet=50412). ab27484 with ab45678 Click here (or use the following: https://www.abcam.com/index.html?datasheet=27484). Please do not hesitate to contact me again with any further questions or if you consider testing one of the latter antibodies in IHC-P.