Overview

  • Product name
    Anti-Choline Acetyltransferase antibody
    See all Choline Acetyltransferase primary antibodies
  • Description
    Rabbit polyclonal to Choline Acetyltransferase
  • Host species
    Rabbit
  • Specificity
    Extensive immunohistochemistry indicates localisation to cholinergic nervous system only. Will stain cholinergic neurons in rat central and peripheral nervous systems.
  • Tested applications
    Suitable for: ICC/IF, ELISA, IHC-P, IHC-Fr, IHC-FrFlmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Guinea pig
    Predicted to work with: Chicken, Human, Pig, Non human primatesDoes not react with: Sheep
  • Immunogen

    Synthetic peptide:

    H-GLFSSYRLPGHTQDTLVAQKSS

    conjugated to KLH by a Glutaraldehyde linker, corresponding to amino acids 168-189 of Choline Acetyltransferase.

  • Positive control
    • IHC: Rat forebrain tissue.
  • General notes
    This antibody appears to have identical characteristics to those described by Benecke et al (1993). The antigen is the same. It is an excellent reagent for immunohistochemistry for both peripheral and central nervous system, staining both cell bodies and nerve terminals.

Properties

Applications

Our Abpromise guarantee covers the use of ab6168 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration. See Abreview.
ELISA 1/1000.
IHC-P Use at an assay dependent concentration. PubMed: 19422789
IHC-Fr 1/2000. Fix using formaldehyde. Perfusion fix the tissue with Zamboni fixative and post-fix (1-2hrs according to Masliukov PM & Timmermans) in 4% formaldehyde (4% formaldehyde with or without 15% picric acid is a very good fixative, it should also work with Bouin fixative too).Cryoprotect the tissue by direct immersion in 30% sucrose (at +4C). Use on free floating sections for best results.
This antibody does not work with glutaraldehyde fixation.
IHC-FrFl Use at an assay dependent concentration.

Target

Images

  • Immunohistochemistry analysis of free-floating rat forebrain tissue labelling Choline Acetyltransferase with ab6168 at a dilution of 1/100.

  • ab6168 staining rat stomach tissue sections by immunohistochemistry (Frozen sections). Tissue underwent fixation in paraformaldehyde. The primary antibody was diluted 1/200 and incubated with sample for 24 hours. A Texas Red® conjugated goat polyclonal (IgG whole molecule) to rabbit IgG, diluted 1/400 was used  as secondary. 

    See Abreview

References

This product has been referenced in:
  • Bagher Z  et al. Differentiation of neural crest stem cells from nasal mucosa into motor neuron-like cells. J Chem Neuroanat 92:35-40 (2018). ICC/IF . Read more (PubMed: 29807106) »
  • Chen X  et al. Functional Multichannel Poly(Propylene Fumarate)-Collagen Scaffold with Collagen-Binding Neurotrophic Factor 3 Promotes Neural Regeneration After Transected Spinal Cord Injury. Adv Healthc Mater 7:e1800315 (2018). Read more (PubMed: 29920990) »
See all 7 Publications for this product

Customer reviews and Q&As

Filter by Application

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1-7 of 7 Abreviews

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (human Mueller cells (MIO-M1))
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate pH 6
Permeabilization
No
Specification
human Mueller cells (MIO-M1)
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
10% NBF

Abcam user community

Verified customer

Submitted Feb 17 2016

Application
Western blot
Sample
Rhesus monkey Tissue lysate - whole (macaque retina)
Gel Running Conditions
Reduced Denaturing (4-15%)
Loading amount
100 µg
Specification
macaque retina
Blocking step
Milk as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: 25°C

Monica Sauter

Verified customer

Submitted Jan 11 2016

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Cynomolgus Monkey Tissue sections (macaque retina)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate pH 6
Permeabilization
No
Specification
macaque retina
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Dec 29 2015

Application
Immunohistochemistry (Frozen sections)
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 21°C
Sample
Mouse Tissue sections (Brain)
Specification
Brain
Permeabilization
Yes - 0.25 % Triton-x ind 0.1M PB
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Sep 10 2013

Application
Immunohistochemistry (Frozen sections)
Sample
Rat Tissue sections (Stomach)
Specification
Stomach
Fixative
Paraformaldehyde
Permeabilization
No

Prof. Marcin Arciszewski

Verified customer

Submitted Oct 27 2008

Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (Brain sections)
Specification
Brain sections
Fixative
Paraformaldehyde
Permeabilization
No
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C

Dr. Li-Hsien Lin

Verified customer

Submitted Oct 10 2008

This product is known to not work in this application or species.
Application
Immunohistochemistry (Frozen sections)
Sample
Sheep Tissue sections (pancreas)
Specification
pancreas
Fixative
Paraformaldehyde
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.1

Prof. Marcin Arciszewski

Verified customer

Submitted Feb 22 2006

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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