Recombinant Anti-Choline Acetyltransferase antibody [EPR16590] (ab178850)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR16590] to Choline Acetyltransferase
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Rabbit, Guinea pig
Related conjugates and formulations
Overview
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Product name
Anti-Choline Acetyltransferase antibody [EPR16590]
See all Choline Acetyltransferase primary antibodies -
Description
Rabbit monoclonal [EPR16590] to Choline Acetyltransferase -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Rabbit, Guinea pig -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Rat brain lysate; Rabbit brain lysate; Guinea Pig brain and cerebellum lysates. IHC-P: Mouse cerebral cortex tissue, Guinea Pig cerebral cortex tissue,
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR16590 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
- Anti-Choline Acetyltransferase antibody [EPR16590] - BSA and Azide free (ab221793)
- Alexa Fluor® 647 Anti-Choline Acetyltransferase antibody [EPR16590] (ab225262)
- Alexa Fluor® 488 Anti-Choline Acetyltransferase antibody [EPR16590] (ab225263)
- Alexa Fluor® 568 Anti-Choline Acetyltransferase antibody [EPR16590] (ab313102)
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab178850 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB | (1) |
1/1000. Detects a band of approximately 72 kDa (predicted molecular weight: 72 kDa).
|
IHC-P | (2) |
1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Notes |
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WB
1/1000. Detects a band of approximately 72 kDa (predicted molecular weight: 72 kDa). |
IHC-P
1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Target
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Function
Catalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses. -
Involvement in disease
Myasthenic syndrome, congenital, 6, presynaptic -
Sequence similarities
Belongs to the carnitine/choline acetyltransferase family. - Information by UniProt
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Database links
- Entrez Gene: 100718930 Guinea pig
- Entrez Gene: 12647 Mouse
- Entrez Gene: 100342226 Rabbit
- Entrez Gene: 290567 Rat
- SwissProt: Q03059 Mouse
- SwissProt: P32738 Rat
- Unigene: 442817 Mouse
- Unigene: 45116 Rat
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Alternative names
- Acetyl CoA choline O acetyltransferase antibody
- Acetyl CoA:choline O acetyltransferase antibody
- ChAT antibody
see all
Images
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Immunohistochemical analysis of paraffin-embedded Guinea Pig cerebral cortex tissue labeling Choline Acetyltransferase with ab178850 at 1/2000 followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Nucleus and cytoplasm staining on neurons of Guinea Pig cerebral cortex is observed. Counter stained with Hematoxylin.
Negative control: Using PBS instead of primary ab, secondary ab is prediluted HRP Polymer for Rabbit/Mouse IgG.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin-embedded Mouse cerebral cortex tissue labeling Choline Acetyltransferase with ab178850 at 1/2000 followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Nucleus and cytoplasm staining on neurons of Mouse cerebral cortex is observed. Counter stained with Hematoxylin.
Negative control: Using PBS instead of primary ab, secondary ab is prediluted HRP Polymer for Rabbit/Mouse IgG.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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All lanes : Anti-Choline Acetyltransferase antibody [EPR16590] (ab178850) at 1/5000 dilution
Lane 1 : Guinea Pig brain lysate
Lane 2 : Guinea Pig cerebellum lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 72 kDa
Observed band size: 72 kDaBlocking/Dilution buffer: 5% NFDM/TBST.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse brain tissue sections labeling Choline Acetyltransferase with ab178850 at 1/2000 dilution. Cells were fixed in 10% normal buffered formalin, heat mediated antigen retrieval was performed using TE pH9. A donkey anti-rabbit Alexa Fluor 647 was used as the secondary antibody at 1/300 dilution. Green was a false colour used to represent the Alexa Fluor 647 fluorescent signal.
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Anti-Choline Acetyltransferase antibody [EPR16590] (ab178850) at 1/1000 dilution + Rabbit brain lysate at 10 µg
Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 72 kDa
Observed band size: 72 kDaBlocking/Dilution buffer: 5% NFDM/TBST.
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Anti-Choline Acetyltransferase antibody [EPR16590] (ab178850) at 1/1000 dilution + Rat brain lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution
Predicted band size: 72 kDa
Observed band size: 72 kDaBlocking/Dilution buffer: 5% NFDM/TBST.
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Anti-Choline Acetyltransferase antibody [EPR16590] (ab178850) at 1/2000 dilution + Mouse brain tissue lysate at 20 µg with 5% NFDM/TBST
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 72 kDa
Observed band size: 72 kDa
Exposure time: 3 minutes
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (39)
ab178850 has been referenced in 39 publications.
- Yoon EJ et al. Improvement of Cognitive Function in Ovariectomized Rats by Human Neural Stem Cells Overexpressing Choline Acetyltransferase via Secretion of NGF and BDNF. Int J Mol Sci 23:N/A (2022). PubMed: 35628371
- Qian XH et al. Injection of amyloid-β to lateral ventricle induces gut microbiota dysbiosis in association with inhibition of cholinergic anti-inflammatory pathways in Alzheimer's disease. J Neuroinflammation 19:236 (2022). PubMed: 36171620
- Mehla J et al. Dramatic impacts on brain pathology, anxiety, and cognitive function in the knock-in APPNL-G-F mouse model of Alzheimer disease following long-term voluntary exercise. Alzheimers Res Ther 14:143 (2022). PubMed: 36180883
- Yang Y et al. Single-Cell Transcriptional Profiling Reveals Low-Level Tragus Stimulation Improves Sepsis-Induced Myocardial Dysfunction by Promoting M2 Macrophage Polarization. Oxid Med Cell Longev 2022:3327583 (2022). PubMed: 36285297
- Chen YA et al. In vivo genome editing using novel AAV-PHP variants rescues motor function deficits and extends survival in a SOD1-ALS mouse model. Gene Ther N/A:1-12 (2022). PubMed: 36450833