Recombinant
RabMAb

Recombinant Anti-Choline Acetyltransferase antibody [EPR16590] (ab178850)

Overview

  • Product name
    Anti-Choline Acetyltransferase antibody [EPR16590]
    See all Choline Acetyltransferase primary antibodies
  • Description
    Rabbit monoclonal [EPR16590] to Choline Acetyltransferase
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Rabbit, Guinea pig
  • Immunogen

    Synthetic peptide within Pig Choline Acetyltransferase aa 150-250. The exact sequence is proprietary.
    Database link: P13222
    (Peptide available as ab203108)

  • Positive control
    • WB: Rat brain lysate; Rabbit brain lysate; Guinea Pig brain and cerebellum lysates. IHC-P: Mouse cerebral cortex tissue, Guinea Pig cerebral cortex tissue,
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Monoclonal
  • Clone number
    EPR16590
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab178850 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 72 kDa (predicted molecular weight: 72 kDa).
IHC-P 1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

Target

  • Function
    Catalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses.
  • Involvement in disease
    Myasthenic syndrome, congenital, 6, presynaptic
  • Sequence similarities
    Belongs to the carnitine/choline acetyltransferase family.
  • Information by UniProt
  • Database links
  • Alternative names
    • Acetyl CoA choline O acetyltransferase antibody
    • Acetyl CoA:choline O acetyltransferase antibody
    • Chat antibody
    • CHOACTase antibody
    • Choline acetylase antibody
    • choline acetyltransferase antibody
    • Choline O acetyltransferase antibody
    • Choline O-acetyltransferase antibody
    • CLAT_HUMAN antibody
    • CMS1A antibody
    • CMS1A2 antibody
    • EC 2.3.1.6 antibody
    • OTTHUMP00000019583 antibody
    • OTTHUMP00000019584 antibody
    see all

Images

  • Immunohistochemical analysis of paraffin-embedded Guinea Pig cerebral cortex tissue labeling Choline Acetyltransferase with ab178850 at 1/2000 followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Nucleus and cytoplasm staining on neurons of Guinea Pig cerebral cortex is observed. Counter stained with Hematoxylin.

    Negative control: Using PBS instead of primary ab, secondary ab is prediluted HRP Polymer for Rabbit/Mouse IgG.

  • Immunohistochemical analysis of paraffin-embedded Mouse cerebral cortex tissue labeling Choline Acetyltransferase with ab178850 at 1/2000 followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Nucleus and cytoplasm staining on neurons of Mouse cerebral cortex is observed. Counter stained with Hematoxylin.

    Negative control: Using PBS instead of primary ab, secondary ab is prediluted HRP Polymer for Rabbit/Mouse IgG.

  • All lanes : Anti-Choline Acetyltransferase antibody [EPR16590] (ab178850) at 1/5000 dilution

    Lane 1 : Guinea Pig brain lysate
    Lane 2 : Guinea Pig cerebellum lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 72 kDa
    Observed band size: 72 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse brain tissue sections labeling Choline Acetyltransferase with ab178850 at 1/2000 dilution. Cells were fixed in 10% normal buffered formalin, heat mediated antigen retrieval was performed using TE pH9. A donkey anti-rabbit Alexa Fluor 647 was used as the secondary antibody at 1/300 dilution. Green was a false colour used to represent the Alexa Fluor 647 fluorescent signal. 

    See Abreview

  • Anti-Choline Acetyltransferase antibody [EPR16590] (ab178850) at 1/1000 dilution + Rabbit brain lysate at 10 µg

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 72 kDa
    Observed band size: 72 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Anti-Choline Acetyltransferase antibody [EPR16590] (ab178850) at 1/1000 dilution + Rat brain lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 72 kDa
    Observed band size: 72 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Anti-Choline Acetyltransferase antibody [EPR16590] (ab178850) at 1/2000 dilution + Mouse brain tissue lysate at 20 µg with 5% NFDM/TBST

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 72 kDa
    Observed band size: 72 kDa


    Exposure time: 3 minutes

References

This product has been referenced in:
  • Wang TH  et al. Fisetin Exerts Antioxidant and Neuroprotective Effects in Multiple Mutant hSOD1 Models of Amyotrophic Lateral Sclerosis by Activating ERK. Neuroscience 379:152-166 (2018). IHC-P . Read more (PubMed: 29559385) »
  • Hixon AM  et al. A mouse model of paralytic myelitis caused by enterovirus D68. PLoS Pathog 13:e1006199 (2017). Read more (PubMed: 28231269) »
See all 3 Publications for this product

Customer reviews and Q&As

Filter by Application

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1-4 of 4 Abreviews

Application
IHC - Wholemount
Sample
Asytanax mexicanus Embryo (larvae 12 days post fertilization)
Specification
larvae 12 days post fertilization

Abcam user community

Verified customer

Submitted Aug 24 2018

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Cynomolgus monkey Tissue sections (Brain)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: pH 9.0 Tris-EDTA
Permeabilization
No
Specification
Brain
Blocking step
Protein Block (ab64226) as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: RT°C
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Jan 09 2018

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Brain)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: TE pH9
Permeabilization
No
Specification
Brain
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative
10% Normal buffered formalin

Abcam user community

Verified customer

Submitted Oct 21 2016

Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Mouse Tissue sections (Spinal Cord)
Antigen retrieval step
None
Permeabilization
Yes - 0.3% PBS-X
Specification
Spinal Cord
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Periodate/lysine/paraformaldehyde

Abcam user community

Verified customer

Submitted Feb 23 2016

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