Product nameAnti-Choline Acetyltransferase antibody [EPR16590] (Alexa Fluor® 647)
See all Choline Acetyltransferase primary antibodies
DescriptionRabbit monoclonal [EPR16590] to Choline Acetyltransferase (Alexa Fluor® 647)
ConjugationAlexa Fluor® 647. Ex: 652nm, Em: 668nm
Tested applicationsSuitable for: IHC-Pmore details
Species reactivityReacts with: Mouse
Predicted to work with: Rat, Rabbit, Guinea pig
Synthetic peptide within Pig Choline Acetyltransferase aa 150-250. The exact sequence is proprietary.
Database link: P13222
- IHC-P: normal mouse brain tissue sections
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or email@example.com.
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 30% Glycerol, 1% BSA, PBS
Concentration information loading...
PurityProtein A purified
- Anti-Choline Acetyltransferase antibody [EPR16590] (ab178850)
- Anti-Choline Acetyltransferase antibody [EPR13024(B)] (ab181023)
- Anti-Choline Acetyltransferase antibody (ab18736)
- Anti-Choline Acetyltransferase antibody [EPR13024(B)] (Alexa Fluor® 488) (ab192465)
- Anti-Choline Acetyltransferase antibody [EPR13024(B)] (Alexa Fluor® 647) (ab192466)
- Anti-Choline Acetyltransferase antibody [EPR16590] - BSA and Azide free (ab221793)
- Anti-Choline Acetyltransferase antibody [EPR13024(B)] (Phycoerythrin) (ab224000)
- Anti-Choline Acetyltransferase antibody [EPR13024(B)] - BSA and Azide free (ab224267)
- Anti-Choline Acetyltransferase antibody [EPR16590] (Alexa Fluor® 488) (ab225263)
Our Abpromise guarantee covers the use of ab225262 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionCatalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses.
Involvement in diseaseMyasthenic syndrome, congenital, 6, presynaptic
Sequence similaritiesBelongs to the carnitine/choline acetyltransferase family.
- Information by UniProt
- Acetyl CoA choline O acetyltransferase antibody
- Acetyl CoA:choline O acetyltransferase antibody
- ChAT antibody
IHC image of Choline Acetyltransferase staining in a section of formalin-fixed paraffin-embedded normal mouse brain.
The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH9, epitope retrieval solution 2) for 20mins, performed on a Leica BOND™. Non-specific protein-protein interactions were then blocked in TBS containing 0.025% (v/v) Triton X-100, 0.3M (w/v) glycine and 1% (w/v) BSA for 1h at room temperature. The section was then incubated overnight at +4°C in TBS containing 0.025% (v/v) Triton X-100 and 1% (w/v) BSA with ab225262 at 1/100 dilution (shown in red) and counterstained using ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Fluoromount®.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times.
ab225262 has not yet been referenced specifically in any publications.