Recombinant Anti-Choline Acetyltransferase antibody [EPR16590] - BSA and Azide free (ab221793)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR16590] to Choline Acetyltransferase - BSA and Azide free
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Rabbit, Guinea pig
Related conjugates and formulations
Overview
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Product name
Anti-Choline Acetyltransferase antibody [EPR16590] - BSA and Azide free
See all Choline Acetyltransferase primary antibodies -
Description
Rabbit monoclonal [EPR16590] to Choline Acetyltransferase - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Rabbit, Guinea pig -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Rat brain lysate; Rabbit brain lysate; Guinea Pig brain and cerebellum lysates. IHC-P: Mouse cerebral cortex tissue, Guinea Pig cerebral cortex tissue,
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General notes
ab221793 is the carrier-free version of ab178850.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR16590 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
- Anti-Choline Acetyltransferase antibody [EPR16590] (ab178850)
- Alexa Fluor® 647 Anti-Choline Acetyltransferase antibody [EPR16590] (ab225262)
- Alexa Fluor® 488 Anti-Choline Acetyltransferase antibody [EPR16590] (ab225263)
- Alexa Fluor® 594 Anti-Choline Acetyltransferase antibody [EPR16590] (ab311819)
- Alexa Fluor® 568 Anti-Choline Acetyltransferase antibody [EPR16590] (ab313102)
- Alexa Fluor® 555 Anti-Choline Acetyltransferase antibody [EPR16590] (ab313295)
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Compatible Secondaries
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Conjugation kits
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab221793 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
Use at an assay dependent concentration. Detects a band of approximately 72 kDa (predicted molecular weight: 72 kDa).
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IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ab199376 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Notes |
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WB
Use at an assay dependent concentration. Detects a band of approximately 72 kDa (predicted molecular weight: 72 kDa). |
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. ab199376 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Target
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Function
Catalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses. -
Involvement in disease
Myasthenic syndrome, congenital, 6, presynaptic -
Sequence similarities
Belongs to the carnitine/choline acetyltransferase family. - Information by UniProt
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Database links
- Entrez Gene: 100718930 Guinea pig
- Entrez Gene: 12647 Mouse
- Entrez Gene: 100342226 Rabbit
- Entrez Gene: 290567 Rat
- SwissProt: Q03059 Mouse
- SwissProt: P32738 Rat
- Unigene: 442817 Mouse
- Unigene: 45116 Rat
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Alternative names
- Acetyl CoA choline O acetyltransferase antibody
- Acetyl CoA:choline O acetyltransferase antibody
- ChAT antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse brain tissue sections labeling Choline Acetyltransferase with ab178850 at 1/2000 dilution. Cells were fixed in 10% normal buffered formalin, heat mediated antigen retrieval was performed using TE pH9. A donkey anti-rabbit Alexa Fluor 647 was used as the secondary antibody at 1/300 dilution. Green was a false colour used to represent the Alexa Fluor 647 fluorescent signal.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab178850).
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Immunohistochemical analysis of paraffin-embedded Guinea Pig cerebral cortex tissue labeling Choline Acetyltransferase with ab178850 at 1/2000 followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Nucleus and cytoplasm staining on neurons of Guinea Pig cerebral cortex is observed. Counter stained with Hematoxylin.
Negative control: Using PBS instead of primary ab, secondary ab is prediluted HRP Polymer for Rabbit/Mouse IgG.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab178850).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin-embedded Mouse cerebral cortex tissue labeling Choline Acetyltransferase with ab178850 at 1/2000 followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Nucleus and cytoplasm staining on neurons of Mouse cerebral cortex is observed. Counter stained with Hematoxylin.
Negative control: Using PBS instead of primary ab, secondary ab is prediluted HRP Polymer for Rabbit/Mouse IgG.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab178850).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Protocols
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (1)
ab221793 has been referenced in 1 publication.
- Farhadi A et al. P38 initiates degeneration of midbrain GABAergic and glutamatergic neurons in diabetes models. Eur J Neurosci 56:3755-3778 (2022). PubMed: 35513862