Recombinant
RabMAb

Recombinant Anti-Choline Acetyltransferase antibody [EPR16590] - BSA and Azide free (ab221793)

Overview

  • Product name

    Anti-Choline Acetyltransferase antibody [EPR16590] - BSA and Azide free
    See all Choline Acetyltransferase primary antibodies
  • Description

    Rabbit monoclonal [EPR16590] to Choline Acetyltransferase - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Rabbit, Guinea pig
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Pig Choline Acetyltransferase aa 150-250. The exact sequence is proprietary.
    Database link: P13222

  • Positive control

    • WB: Rat brain lysate; Rabbit brain lysate; Guinea Pig brain and cerebellum lysates. IHC-P: Mouse cerebral cortex tissue, Guinea Pig cerebral cortex tissue,
  • General notes

    Ab221793 is the carrier-free version of ab178850. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab221793 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Detects a band of approximately 72 kDa (predicted molecular weight: 72 kDa).
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

ab199376 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

Target

  • Function

    Catalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses.
  • Involvement in disease

    Myasthenic syndrome, congenital, 6, presynaptic
  • Sequence similarities

    Belongs to the carnitine/choline acetyltransferase family.
  • Information by UniProt
  • Database links

  • Alternative names

    • Acetyl CoA choline O acetyltransferase antibody
    • Acetyl CoA:choline O acetyltransferase antibody
    • ChAT antibody
    • CHOACTase antibody
    • Choline acetylase antibody
    • choline acetyltransferase antibody
    • Choline O acetyltransferase antibody
    • Choline O-acetyltransferase antibody
    • CLAT_HUMAN antibody
    • CMS1A antibody
    • CMS1A2 antibody
    • EC 2.3.1.6 antibody
    • OTTHUMP00000019583 antibody
    • OTTHUMP00000019584 antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse brain tissue sections labeling Choline Acetyltransferase with ab178850 at 1/2000 dilution. Cells were fixed in 10% normal buffered formalin, heat mediated antigen retrieval was performed using TE pH9. A donkey anti-rabbit Alexa Fluor 647 was used as the secondary antibody at 1/300 dilution. Green was a false colour used to represent the Alexa Fluor 647 fluorescent signal. 

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab178850).

  • Immunohistochemical analysis of paraffin-embedded Guinea Pig cerebral cortex tissue labeling Choline Acetyltransferase with ab178850 at 1/2000 followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Nucleus and cytoplasm staining on neurons of Guinea Pig cerebral cortex is observed. Counter stained with Hematoxylin.

    Negative control: Using PBS instead of primary ab, secondary ab is prediluted HRP Polymer for Rabbit/Mouse IgG.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab178850).

  • Immunohistochemical analysis of paraffin-embedded Mouse cerebral cortex tissue labeling Choline Acetyltransferase with ab178850 at 1/2000 followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Nucleus and cytoplasm staining on neurons of Mouse cerebral cortex is observed. Counter stained with Hematoxylin.

    Negative control: Using PBS instead of primary ab, secondary ab is prediluted HRP Polymer for Rabbit/Mouse IgG.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab178850).

References

ab221793 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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