Anti-Choline kinase alpha antibody (ab235938)
Key features and details
- Rabbit polyclonal to Choline kinase alpha
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Choline kinase alpha antibody
See all Choline kinase alpha primary antibodies -
Description
Rabbit polyclonal to Choline kinase alpha -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human Choline kinase alpha aa 79-173.
Database link: P35790 -
Positive control
- WB: MCF7 whole cell lysate. IHC-P: Human lung cancer tissue. ICC/IF: HepG2 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.03% Proclin 300
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
Purity >95% -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab235938 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/5000. Predicted molecular weight: 52 kDa.
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IHC-P |
1/20 - 1/200.
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ICC/IF |
1/50 - 1/200.
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Notes |
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WB
1/500 - 1/5000. Predicted molecular weight: 52 kDa. |
IHC-P
1/20 - 1/200. |
ICC/IF
1/50 - 1/200. |
Target
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Relevance
The dominant pathway for biosynthesis of phosphatidylcholine occurs through the CDP-choline pathway. Choline kinase alpha (CHK) is the first enzyme in the pathway and may thereby play an upstream regulatory role in lipid transport and metabolism. CHK additionally catalyzes phosphorylation of ethanolamine. -
Cellular localization
Cytoplasmic -
Database links
- Entrez Gene: 1119 Human
- Omim: 118491 Human
- SwissProt: P35790 Human
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Alternative names
- CHETK alpha antibody
- CHETK-alpha antibody
- CHK antibody
see all
Images
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Anti-Choline kinase alpha antibody (ab235938) at 1/500 dilution + MCF7 (human breast adenocarcinoma cell line) whole cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 52 kDa -
Paraffin-embedded human lung cancer tissue stained for Choline kinase alpha using ab235938 at 1/100 dilution in immunohistochemical analysis.
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4% formaldehyde-fixed, 0.2% Triton X-100 permeabilized HepG2 (human liver hepatocellular carcinoma cell line) cells stained for Choline kinase alpha (green) using ab235938 at 1/100 dilution in ICC/IF. Secondary antibody is Alexa Fluor® 488-congugated Goat Anti-Rabbit IgG (H+L).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab235938 has not yet been referenced specifically in any publications.