Overview

  • Product name

    Choline Kinase Assay Kit
  • Detection method

    Fluorescent
  • Sample type

    Cell Lysate, Purified protein, Tissue Lysate
  • Product overview

    In Choline Kinase Assay Kit (ab239729), choline is phosphorylated by choline kinase producing a series of intermediates, which react with the fluorometric probe generating a strong flurorescence signal (Ex/Em = 535/587 nm). The signal is directly proportional to choline kinase activity present in the biological sample. The assay is fast, sensitive, reproducible and is suitable for measuring choline kinase activity in different cells and other biological samples This kit can detect as low as 0.5 μM of choline kinase activity.

  • Platform

    Microplate reader

Properties

  • Storage instructions

    Please refer to protocols.
  • Components 100 tests
    ADP Standard (1 µmole) 1 vial
    Assay Buffer 1 x 50ml
    ATP 1 vial
    Detection Mix I 1 vial
    Detection Mix II 1 vial
    Human ChoK 1 x 10µl
    Probe 1 x 0.4ml
    Substrate 1 vial
  • Relevance

    Choline kinase is an enzyme that catalyzes the first reaction in the CDP-choline pathway for the synthesis of phosphatidylcholine, a membrane phospholipid. This transferase, utilizes Mg2+ as a cofactor in order to transfer a phosphate moiety from ATP to choline molecules forming phosphocholine. In humans, there are two isozymes, Choline Kinase A and B. Both proteins share more than 60% in homology. Choline Kinase plays pivotal roles in different human diseases: elevated Choline kinase A expression has been found in breast, lung, colorectal, prostate and bladder tumor tissues whereas choline kinase B is suggested to be involved in muscular dystrophy.

Images

Protocols

References

ab239729 has not yet been referenced specifically in any publications.

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