Choline/Acetylcholine Assay Kit (ab65345)
- Datasheet
- Specific References (10)
- Protocols
Overview
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Product nameCholine/Acetylcholine Assay Kit
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Sample typeCell culture supernatant, Urine, Serum, Plasma, Cell culture extracts, Other biological fluids, Tissue Extracts
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Assay typeQuantitative
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Sensitivity> 0.01 nmol/well
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Range0.01 nmol/well - 5 nmol/well
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Assay time0h 40m
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Species reactivityReacts with: Mammals, Other species
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Product overview
Choline/Acetylcholine Assay Kit (ab65345) provides a simple and sensitive means for quantifying Choline and Acetylcholine by either a colorimetric or fluorometric method. In the assay free choline is oxidized to betaine, via the intermediate betaine aldehyde. The reaction generates products which react with the Choline Probe to generate color (λ= 570 nm), and fluorescence (Ex/Em 535/587 nm). Acetylcholine can be converted to choline by adding acetylcholinesterase to the reaction. The kit can detect choline and acetylcholine (total choline – free choline) in various biological samples such as in blood, cells, culture media, fermentation media, etc. There is no need for pretreatment or purification of samples. The kit can detect ~10 pmol-5 nmol of choline or acetylcholine.
Cells should be cultured in choline chloride free medium.
Visit our FAQs page for tips and troubleshooting. -
Notes
Choline and acetylcholine play important roles in many biological processes. Choline is an essential nutrient, commonly grouped with the B complex vitamins, that plays key roles in many biological processes. Choline is a precursor for the synthesis of acetylcholine (AchE), a critical neurotransmitter in both the periferial and central nervous systems.
If you need to purchase additional Choline Probe for this product, please see ab139505.
Properties
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Storage instructionsStore at -20°C. Please refer to protocols.
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Storage bufferPreservative: None
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Components Identifier 100 tests Acetylcholinesterase Blue 1 vial Choline Assay Buffer WM 1 x 25ml Choline Enzyme Mix Green 1 vial Choline Probe (in DMSO) Red 1 x 200µl Choline Standard (5 µmol) Yellow 1 vial -
Research areas
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RelevanceCholine and acetylcholine (often abbreviated ACh) play important roles in many biological processes. Choline is a natural amine, and is generally found in phosphatidylcholine and sphingomyelin, phopholipids abundant in cell membranes. Choline is also the precursor molecule for the neurotransmitter acetylcholine which is involved in many functions both in the peripheral nervous system (PNS) and in the central nervous system (CNS). In the PNS, ACh activates muscles and is a major neurotransmitter in the autonomic nervous system. In the CNS, ACh and the associated neurons form the cholinergic system, which tends to cause anti-excitatory actions.
Images
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Functional studies- ab65345Image from Kim J.H et al., PLoS One 8(2), Fig 4. doi: 10.1371/journal.pone.0056736.Acetylcholine levels from mouse brain tissue was measured using Acetylcholine assay kit (ab65345). Normal mice were anesthetized with thiopental sodium at 20minutes after the end of electroacupuncture (EA) treatment to determine levels of ACh (Figure A). Immunohistochemical staining mAChR (muscarinic acetylcholine receptor) M3 in mouse cerebral cortex tissue 20 minutes after the end of EA treatment (Image B).
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Functional Studies - Choline/Acetylcholine Assay Kit (ab65345) -
Functional Studies - Choline/Acetylcholine Assay Kit (ab65345)Choline measured fluorometrically in mouse tissue lysates showing quantity (nmol) per mg protein of tested sample
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Functional Studies - Choline/Acetylcholine Assay Kit (ab65345)Choline measured fluorometrically in cell lysates showing quantity (pmol) per 1 mln of tested cells
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Functional Studies - Choline/Acetylcholine Assay Kit (ab65345)Choline measured fluorometrically in biological fluids showing quantity (nmol) per mL of tested sample
Protocols
References
This product has been referenced in:
- Garrido-Gil P et al. Aging-related dysregulation in enteric dopamine and angiotensin system interactions: implications for gastrointestinal dysfunction in the elderly. Oncotarget 9:10834-10846 (2018). Functional Studies . Read more (PubMed: 29541380) »
- van Loo B et al. Structural and Mechanistic Analysis of the Choline Sulfatase from Sinorhizobium melliloti: A Class I Sulfatase Specific for an Alkyl Sulfate Ester. J Mol Biol 430:1004-1023 (2018). Read more (PubMed: 29458126) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"