Anti-Chondroitin Sulfate antibody [CS-56] (ab11570)

Reviews (3)Q&A (7)References (31)

Overview

  • Product name

    Anti-Chondroitin Sulfate antibody [CS-56]

  • Description

    Mouse monoclonal [CS-56] to Chondroitin Sulfate

  • Host species

    Mouse

  • Specificity

    The antibody has been reported to be specific for the glycosaminoglycan (GAG) portion of native chondroitin sulfate proteoglycan (CSPG). The antibody reacts specifically with chondroitin sulfate types A and C but not with type B (dermatan sulfate), and may be used for localization of chondroitin sulfate in tissues and cultured fibroblasts.

  • Tested applications

    Suitable for: Electron Microscopy, IHC-Fr, IHC-P, ICC/IFmore details

  • Species reactivity

    Reacts with: Mouse, Chicken, Cow, Human, Apteronotus leptorhynchus

  • Immunogen

    Ventral membranes of chicken gizzard fibroblasts.

  • Positive control

    • Bovine mammary gland epithelial (BMGE) cells.

  • General notes

    This product was changed from ascites to tissue culture supernatant on 25th October 2016. The following lot(s) is/are from ascites and is still in stock as of 25th October 2016 - GR288674, GR272407. Lot numbers higher than GR288674, GR272407 will be from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly

    Storage in frost-free freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

    Many cellular activities depend on the interaction of cells with the surrounding extracellular matrix (ECM). Most cells, in intact tissue and in culture, are attached to an ECM. Epithelial cells are associated with the basement membrane; fibroblastic cells are usually embedded in a pericellular mesh of fibrils, and tissue culture cells usually grow on a substrate which is covered by various ECM components. Studies have indicated that the matrix or its various isolated components provide not only adhesive surfaces for cells to grow on but also have effects on the rate of cell growth, mobility, morphogenesis and differentiation. Within the ECM several glycoproteins and proteoglycans have been identified. It has been proposed that the different constituents interact with each other in a rather complex fashion. The poor antigenicity of proteoglycans especially their glycoaminoglycan (GAG) moieties make it difficult to localize these molecules in tissue and cell culture. Monoclonal Anti-Chondroitin Sulfate can be used to study chondroitin sulfate proteoglycan (CSPG) distribution and its relationships to specific cell-substrate contacts.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab11570 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Electron Microscopy Use at an assay dependent concentration.
IHC-Fr 1/200. PubMed: 16237170
The antibody has been reported to be specific for the glycosaminoglycan (GAG) portion of native chondroitin sulfate proteoglycan (CSPG). The antibody reacts specifically with chondroitin sulfate types A and C but not with type B (dermatan sulfate), and may be used for localization of chondroitin sulfate in tissues and cultured fibroblasts.
IHC-P Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Chondroitin Sulfate antibody [CS-56] (ab11570)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Chondroitin Sulfate antibody [CS-56] (ab11570)Image courtesy of Adrian Epstein by Abreview.
    ab11570 staining Chondroitin Sulfate in murine brain tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
    For more detail please see abreview.

    See Abreview

  • Immunohistochemistry (Frozen sections) - Anti-Chondroitin Sulfate antibody [CS-56] (ab11570)
    Immunohistochemistry (Frozen sections) - Anti-Chondroitin Sulfate antibody [CS-56] (ab11570)Image courtesy of Dr Ruxandra Sirbulescu by Abreview.
    ab11570 staining Chondroitin Sulfate in 16 µm thick transverse sections of Apteronotus leptorhynchus whole body tissue by Immunohistochemistry (Frozen sections).
    Tissue was fixed in 2% paraformaldehyde, blocked with 3% sheep serum, 1% BSA, 1% teleostean gelatine in TBS for 1 hour at 24°C and then incubated with ab11570 at a 1/50 dilution for 18 hours at 4°C. The secondary used was an Alexa-Fluor 546 conjugated goat anti-mouse polyclonal used at a 1/200 dilution.

    See Abreview

  • Immunohistochemistry (Frozen sections) - Anti-Chondroitin Sulfate antibody [CS-56] (ab11570)
    Immunohistochemistry (Frozen sections) - Anti-Chondroitin Sulfate antibody [CS-56] (ab11570)
    Immunoperoxidase staining of a frozen section of normal rabbit aorta stained using Monoclonal Anti-Chondroitin Sulfate ab11570.
  • Electron Microscopy - Anti-Chondroitin Sulfate antibody [CS-56] (ab11570)
    Electron Microscopy - Anti-Chondroitin Sulfate antibody [CS-56] (ab11570)
    Normal rabbit aorta, Lowicryl K4M thin section, stained with Monoclonal Anti-Chondroitin Sulfate, ab11570 and Goat Anti-Mouse IgM (µ-chain specific) 10 nm gold. Counterstain was uranyl acetate and Reynold's lead citrate. Magnification 44,600x. (el=Elastin, col=Collagen).

References

This product has been referenced in:

  • Tessier S  et al. NFAT5/TonEBP controls early acquisition of notochord phenotypic markers, collagen composition, and sonic hedgehog signaling during mouse intervertebral disc embryogenesis. Dev Biol 455:369-381 (2019). Read more (PubMed: 31301300) »
  • Bonavita R  et al. Formation of the sacrum requires down-regulation of sonic hedgehog signaling in the sacral intervertebral discs. Biol Open 7:N/A (2018). Read more (PubMed: 29784673) »
See all 31 Publications for this product

Publishing research using ab11570? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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Western blot abreview for Anti-Chondroitin Sulfate antibody [CS-56]

 Average
Abreviews
abreview image
Application
Western blot
Sample
Apteronotus leptorhynchus Tissue lysate - whole (Brain)
Loading amount
50 µg
Specification
Brain
Gel Running Conditions
Reduced Denaturing (4-15%)
Blocking step
Milk as blocking agent for 14 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Read More

Abcam user community

Verified customer

Submitted Nov 27 2012

Question

Hallo,

wir wuerden gern verschiedene Antikoerper bestellen und haetten zu zwei Antikoerpern noch eine Frage.
1. robo3 (ab77261):
Wir wuerden den Antikoerper gern fuer Immunostainings in Zebrafisch benutzen, leider ist das bei den Applications nicht aufgefuehrt. bedeutet dies das der Antikoerper wirklich nur nicht getestet wurde, oder ist er villeicht nicht fuer diese Anwedung geeignet? Koennen Sie vielleicht eine Aussage trefen wie hoch die Wahrscheinlichkeit ist das er in Immunostainings fuer Zebrafisch ueberhaupt funktioniert, wenn Zebrafisch nur mit "predicted to work in" angegeben ist?
2. Anti-Chondroitin Sulfate antibody (ab11570)
Auch diesen Antikoerper wuerden wir gern in Zebrafisch benutzen. Leider taucht der gar nicht in der Spalte "cross reactivity" auf. auch nicht als "predicted". Koennen sie vielleicht eine Aussage treffen wie ob er uberhaupt mit Zebrafisch reagieren wuerde und wie hoch die Wahrscheinlich keit waere?


Vielen Dank und freundliche Gruesse,

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Answer

Vielen Dank für Ihren Anruf und für Ihr Interesse an unseren Produkten.

Ich freue mich Ihnen bestätigen zu können, dass ab11570 (Anti-Chondroitin Sulfate antibody [CS-56]) für Glykosaminoglykan (GAG) ist, unabhängig von der Spezies. Deshalb kann ich Ihnen diesen Antikörper empfehlen.


Unserem Wissen nach wurde ab77261 (Anti-Robo3 antibody) bisher noch nicht in Zebrafisch und IHC getestet.

Die Wahrscheinlichkeit, dass ab77261 in Zebrafisch funktioniert ist groß, da das Immunogen eine 91% Homologie aufweist.

ab77261 ist auch noch nicht für IHC getestet. Es sieht jedoch vielversprechend aus, da er für ELISA geeignet ist.

Falls Sie dies selbst testen möchten, kann ich Ihnen zurzeit ein spezielles Angebot über einen 100%igen Abreview-Rabatt anbieten. Bei diesem Angebot bekommen Sie einen Rabatt für eine zukünftige Bestellung, wenn Sie uns ein Abreview mit dem Testresultat zusenden. Der Rabatt würde den ganzen Wert von ab77261 abdecken, und gegen eine erneute Bestellung weiterer Produkte von uns verrechnet werden.

Um von diesem Angebot profitieren zu können, folgen Sie bitte diesen Schritten:

1.) Bestätigen Sie mir bitte, dass Sie ab77261 in IHC und/oder Zebrafisch testen möchten.

2.) Bitte bestellen Sie erst nach Erhalt des Rabattcodes.

3.) Bestellen Sie den Antikörper wie üblich per Telefon, Email oder Fax

4.) Testen Sie den Antikörper in IHC und/oder Zebrafisch.

5.) Teilen Sie uns das Ergebnis Ihres Tests durch ein Abreview mit und notieren Sie den Discount Code in dem Feld "additional notes"
Unter der folgenden URL können Sie mehr über unser Abreview System erfahren:
https://www.abcam.com/abreviews

6.) Der Rabattcode ist nach dem Abschicken des Abreviews aktiv, und Sie können weitere Produkte zu dem gleichen Preis wie ab77261 bei uns bestellen (halten Sie bei der Bestellung bitte den Rabattcode). Bitte beachten Sie, dass der Rabattcode innerhalb von 4 Monaten nach Ausstellung eingelöst werden muss.

Der Rabattcode wird gültig unabhängig davon, ob Ihr Ergebnis positiv oder negativ ist.

Die Bedingungen zu unserem 100% Abreview Rabatt können Sie unter dem folgenden Link nachlesen:
https://www.abcam.com/collaborationdiscount

Ich hoffe, diese Informationen helfen Ihnen weiter. Falls Sie einen Rabattcode erhalten möchten oder weitere Fragen haben, zögern Sie bitte nicht, sich wieder an mich zu wenden.

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Question

Inquiry: If the GAG portion of the Chondroitin Sulfate Proteoglycan is enzymaticaly cleaved with Chondroitinase ABC, will the CS-56 still bind to the Proteoglycan core? Will the CS-56 Ab bind to the degradation products of Chondroitinase ABC (D-glucuronic acid and N-acetyl-D-galactosamine)?

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Answer

Unfortunately we have not tested this product with digested chondroitin sulphate and so it is difficult to predict the outcome. GAGs alone are poorly antigenic and so the antibody may not interact with the D-glucuronic acid and N-acetyl-D-galactosamine from the chondroitinase reaction. We have not carried out epitope mapping for this antibody and so again we cannot say for certain whether product ab11570 will bind the proteoglycan core.

I'm sorry I could not provide more definitive information. Please do not hesitate to contact us if you require any further assistance.

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Question

Phone call requesting information on positive control tissue which can be used with the Anti-Chondroitin Sulfate antibody [CS-56] (ab11570).

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Answer

Thank you for contacting us yesterday in regards to the Anti-Chondroitin Sulfate antibody [CS-56] (ab11570).

I have had a look into which tissues may serve as positive controls with the antibody. As the Chondroitin sulfate is a major component of the extracellular matrix I would expect most tissues to show positive staining. I have not been able to find any good references referring to the distribution of the target. As discussed over the phone, we have had reports of brain tissue being used:

https://www.abcam.com/index.html?datasheet=11570&tab=abreviews&intabreviewid=25200

I have also found reference to CS being abundant in fetal liver tissue with lower levels in adult liver tissue:

Gressner AM, Vasel A. Developmental changes of proteoglycan synthesis in rat liver and isolated hepatocytes. Mech Ageing Dev 1985; 31: 307-327

As there are several different chondroitin sulfate proteoglycans such as aggrecan, verican and CD44 it is difficult to determine the exact expression profile but I would expect both the muscle, skinand bone tissue you have access to show some positive staining as indicated from the following references:

http://jcb.rupress.org/content/108/3/1165.full.pdf

http://www.ncbi.nlm.nih.gov/pubmed/11454516

http://dev.biologists.org/content/136/10/e1005.full

I hope this information has been of some help. If you have any further questions, please do not hesitate to ask.

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Question

Is the 1.7mg/ml concentration the IgM concentration or the ascites concentration?

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Answer

Thank you for your enquiry.

The 1.7mg/ml concentration is the antibody concentration which was determined by


Radial immunodiffusion (RID) method. For your information, the ascites concentration with other proteins included is actually 38mg/ml.

I hope this information will be helpful.

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Question

Is this antibody (CS-56) labeled CS could be degraded by chondroitinase ABC?

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Answer

Thank you for contacting us.
Please be advised that ab11570 is not a Chondroitin protein that will be degraded by chondroitinase ABC enzyme. It is an IgM antibody.
I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Read More

Question

Dear Kate,


thank you very much for your kind reply.

Unfortunately I'm not able to send sheep sequence. However I came across one another abcam antibody:Anti-Chondroitin Sulfate antibody [CS-56], (ab11570) which I might use well in my research.

Could you, pleaseconfirm if this antibody reacts with sheep cartilage?

One more question, what kind of predigestion you recommend with this antibody?



Thank you very much!



Best regards,



Andreja

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Answer

Thank you for your reply.

I can confirm that Anti-Chondroitin Sulfate antibody [CS-56], (ab11570) has been raised against the chicken protein. I am sorry this antibody has been sources externally, and regrettably we are not able to obtain the chicken sequence in order to align this with the sheep. The sheep sequence also appears to be unavailable.

For antigen retrieval,there has been an Abreview submitted by another customer who has used a commercially available antigen retrieval solution(Trilogy). I can recommend that antigen retrieval will often require some optimization.You may like to consider trying citrate bufferretrieval pH 6with several time points (3, 5, 10 and 20 minutes) to start with.

We do sell some pre prepared antigen retreival solutions:
https://www.abcam.com/index.html?pageconfig=resource&rid=12854

I am sincerely sorry we have no more information regardingpotential crossreactivity with sheep again on this occasion. If you have any further questions, please do not hesitaet to contact us.

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Question

Phone call: Customer asked for Abreview discount code for using ab118610 and ab11570 in alpha ELISA. - Cells human chondrocytes or bovine chondrocytes  

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Answer

Thank you for contacting us. We need more information on the use of ab11570 and ab8610 in alphaLISA. Could you please answer the following questions? - Are you going to use these antibodies together or in combination with other antibodies? - Are you planning to conjugate these antibodies with different acceptor r donor molecules? - Are you planning to use these antibodies with different conjugated antibodies? e.g acceptor or donor molecule conjugated antibodies. - These antibodies are not tested together in Sandwich ELISA so how you are planning to proceed with the procedure? I am looking forward to hearing from you soon.

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Immunohistochemistry (Frozen sections) abreview for Anti-Chondroitin Sulfate antibody [CS-56]

 Excellent
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Sample
Apteronotus leptorhynchus Tissue sections (Whole body)
Specification
Whole body
Fixative
Paraformaldehyde
Blocking step
3% sheep serum, 1% BSA, 1% teleostean gelatine in TBS as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 24°C
Read More

Dr. Ruxandra Sirbulescu

Verified customer

Submitted Nov 14 2011

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Chondroitin Sulfate antibody [CS-56]

 Good
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Brain)
Specification
Brain
Fixative
microwave euthanasia
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Trilogy
Permeabilization
Yes - tween20 (0.05%)
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C
Read More

Mr. Adrian Epstein

Verified customer

Submitted Aug 02 2011

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