Anti-Chondroitin Sulfate antibody [CS-56] (ab11570)
Key features and details
- Mouse monoclonal [CS-56] to Chondroitin Sulfate
- Suitable for: Electron Microscopy, ICC
- Reacts with: Mouse, Chicken, Cow, Human, Apteronotus leptorhynchus
- Isotype: IgM
Overview
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Product name
Anti-Chondroitin Sulfate antibody [CS-56] -
Description
Mouse monoclonal [CS-56] to Chondroitin Sulfate -
Host species
Mouse -
Specificity
The antibody has been reported to be specific for the glycosaminoglycan (GAG) portion of native chondroitin sulfate proteoglycan (CSPG). The antibody reacts specifically with chondroitin sulfate types A and C but not with type B (dermatan sulfate), and may be used for localization of chondroitin sulfate in tissues and cultured fibroblasts. -
Tested applications
Suitable for: Electron Microscopy, ICCmore details -
Species reactivity
Reacts with: Mouse, Chicken, Cow, Human, Apteronotus leptorhynchus -
Immunogen
Ventral membranes of chicken gizzard fibroblasts.
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Positive control
- Bovine mammary gland epithelial (BMGE) cells.
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General notes
This product was changed from ascites to tissue culture supernatant on 25th October 2016. The following lot(s) is/are from ascites and is still in stock as of 25th October 2016 - GR288674, GR272407. Lot numbers higher than GR288674, GR272407 will be from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly
Storage in frost-free freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.
Many cellular activities depend on the interaction of cells with the surrounding extracellular matrix (ECM). Most cells, in intact tissue and in culture, are attached to an ECM. Epithelial cells are associated with the basement membrane; fibroblastic cells are usually embedded in a pericellular mesh of fibrils, and tissue culture cells usually grow on a substrate which is covered by various ECM components. Studies have indicated that the matrix or its various isolated components provide not only adhesive surfaces for cells to grow on but also have effects on the rate of cell growth, mobility, morphogenesis and differentiation. Within the ECM several glycoproteins and proteoglycans have been identified. It has been proposed that the different constituents interact with each other in a rather complex fashion. The poor antigenicity of proteoglycans especially their glycoaminoglycan (GAG) moieties make it difficult to localize these molecules in tissue and cell culture. Monoclonal Anti-Chondroitin Sulfate can be used to study chondroitin sulfate proteoglycan (CSPG) distribution and its relationships to specific cell-substrate contacts.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Proprietary Purification -
Purification notes
Purified from Tissue culture supernatant. -
Clonality
Monoclonal -
Clone number
CS-56 -
Isotype
IgM -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab11570 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Electron Microscopy |
Use at an assay dependent concentration.
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ICC |
Use at an assay dependent concentration.
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Notes |
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Electron Microscopy
Use at an assay dependent concentration. |
ICC
Use at an assay dependent concentration. |
Images
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Immunocytochemistry analysis of cow mammary gland epithelial cells labeling Chondroitin Sulfate with ab11570 at 2.5 μg/mL. Cells were fixed and permeabilized with 4% Paraformaldehyde and 0.5% Triton X-100. The antibody was developed using Goat Anti-Mouse IgG, Cy3™ conjugate. Cells were counterstained with DAPI (blue) to stain nuclei.
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Normal rabbit aorta, Lowicryl K4M thin section, stained with Monoclonal Anti-Chondroitin Sulfate, ab11570 and Goat Anti-Mouse IgM (µ-chain specific) 10 nm gold. Counterstain was uranyl acetate and Reynold's lead citrate. Magnification 44,600x. (el=Elastin, col=Collagen).
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (38)
ab11570 has been referenced in 38 publications.
- Tessier S et al. Arp2/3 inactivation causes intervertebral disc and cartilage degeneration with dysregulated TonEBP-mediated osmoadaptation. JCI Insight 5:N/A (2020). PubMed: 31961823
- Novais EJ et al. Comparison of inbred mouse strains shows diverse phenotypic outcomes of intervertebral disc aging. Aging Cell 19:e13148 (2020). PubMed: 32319726
- Entchev E et al. Odiparcil, a potential glycosaminoglycans clearance therapy in mucopolysaccharidosis VI-Evidence from in vitro and in vivo models. PLoS One 15:e0233032 (2020). PubMed: 32413051
- Arai Y et al. Tauroursodeoxycholic acid (TUDCA) counters osteoarthritis by regulating intracellular cholesterol levels and membrane fluidity of degenerated chondrocytes. Biomater Sci 7:3178-3189 (2019). PubMed: 31143889
- Tessier S et al. NFAT5/TonEBP controls early acquisition of notochord phenotypic markers, collagen composition, and sonic hedgehog signaling during mouse intervertebral disc embryogenesis. Dev Biol 455:369-381 (2019). PubMed: 31301300