Anti-Chondroitin Sulfate antibody [CS-56] (ab11570)
- Datasheet
- Specific References (20)
- Protocols
Overview
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Product nameAnti-Chondroitin Sulfate antibody [CS-56]
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DescriptionMouse monoclonal [CS-56] to Chondroitin Sulfate
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Host speciesMouse
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SpecificityThe antibody has been reported to be specific for the glycosaminoglycan (GAG) portion of native chondroitin sulfate proteoglycan (CSPG). The antibody reacts specifically with chondroitin sulfate types A and C but not with type B (dermatan sulfate), and may be used for localization of chondroitin sulfate in tissues and cultured fibroblasts.
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Tested applicationsSuitable for: Electron Microscopy, IHC-Fr, IHC-P, ICC/IFmore details
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Species reactivityReacts with: Mouse, Chicken, Cow, Human, Apteronotus leptorhynchus
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Immunogen
Ventral membranes of chicken gizzard fibroblasts.
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Positive control
- Bovine mammary gland epithelial (BMGE) cells.
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General notes
This product was changed from ascites to tissue culture supernatant on 25th October 2016. The following lot(s) is/are from ascites and is still in stock as of 25th October 2016 - GR288674, GR272407. Lot numbers higher than GR288674, GR272407 will be from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly
Storage in frost-free freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.
Many cellular activities depend on the interaction of cells with the surrounding extracellular matrix (ECM). Most cells, in intact tissue and in culture, are attached to an ECM. Epithelial cells are associated with the basement membrane; fibroblastic cells are usually embedded in a pericellular mesh of fibrils, and tissue culture cells usually grow on a substrate which is covered by various ECM components. Studies have indicated that the matrix or its various isolated components provide not only adhesive surfaces for cells to grow on but also have effects on the rate of cell growth, mobility, morphogenesis and differentiation. Within the ECM several glycoproteins and proteoglycans have been identified. It has been proposed that the different constituents interact with each other in a rather complex fashion. The poor antigenicity of proteoglycans especially their glycoaminoglycan (GAG) moieties make it difficult to localize these molecules in tissue and cell culture. Monoclonal Anti-Chondroitin Sulfate can be used to study chondroitin sulfate proteoglycan (CSPG) distribution and its relationships to specific cell-substrate contacts.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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Storage bufferpH: 7.4
Preservative: 0.097% Sodium azide
Constituent: PBS -
Concentration information loading... -
PurityProprietary Purification
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Purification notesPurified from Tissue culture supernatant.
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ClonalityMonoclonal
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Clone numberCS-56
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IsotypeIgM
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Research areas
Associated products
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Compatible Secondaries
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Immunohistochemistry kits
Applications
Our Abpromise guarantee covers the use of ab11570 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Electron Microscopy | Use at an assay dependent concentration. | |
| IHC-Fr | 1/200. PubMed: 16237170 The antibody has been reported to be specific for the glycosaminoglycan (GAG) portion of native chondroitin sulfate proteoglycan (CSPG). The antibody reacts specifically with chondroitin sulfate types A and C but not with type B (dermatan sulfate), and may be used for localization of chondroitin sulfate in tissues and cultured fibroblasts. |
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| IHC-P | Use at an assay dependent concentration. | |
| ICC/IF | Use at an assay dependent concentration. |
Images
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Chondroitin Sulfate antibody [CS-56] (ab11570)](https://www.abcam.com/ps/products/11/ab11570/Images/Chondroitin-Sulfate-Primary-antibodies-ab11570-6.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Chondroitin Sulfate antibody [CS-56] (ab11570)Image courtesy of Adrian Epstein by Abreview.ab11570 staining Chondroitin Sulfate in murine brain tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
For more detail please see abreview. -
![Immunohistochemistry (Frozen sections) - Anti-Chondroitin Sulfate antibody [CS-56] (ab11570)](https://www.abcam.com/ps/products/11/ab11570/Images/Chondroitin-Sulfate-Primary-antibodies-ab11570-15.jpg)
Immunohistochemistry (Frozen sections) - Anti-Chondroitin Sulfate antibody [CS-56] (ab11570)Image courtesy of Dr Ruxandra Sirbulescu by Abreview.ab11570 staining Chondroitin Sulfate in 16 µm thick transverse sections of Apteronotus leptorhynchus whole body tissue by Immunohistochemistry (Frozen sections).
Tissue was fixed in 2% paraformaldehyde, blocked with 3% sheep serum, 1% BSA, 1% teleostean gelatine in TBS for 1 hour at 24°C and then incubated with ab11570 at a 1/50 dilution for 18 hours at 4°C. The secondary used was an Alexa-Fluor 546 conjugated goat anti-mouse polyclonal used at a 1/200 dilution. -
![Immunohistochemistry (Frozen sections) - Anti-Chondroitin Sulfate antibody [CS-56] (ab11570)](https://www.abcam.com/ps/products/11/ab11570/Images/Chondroitin-Sulfate-Primary-antibodies-ab11570-17.jpg)
Immunohistochemistry (Frozen sections) - Anti-Chondroitin Sulfate antibody [CS-56] (ab11570)Immunoperoxidase staining of a frozen section of normal rabbit aorta stained using Monoclonal Anti-Chondroitin Sulfate ab11570. -
![Electron Microscopy - Anti-Chondroitin Sulfate antibody [CS-56] (ab11570)](https://www.abcam.com/ps/products/11/ab11570/Images/Chondroitin-Sulfate-Primary-antibodies-ab11570-18.jpg)
Electron Microscopy - Anti-Chondroitin Sulfate antibody [CS-56] (ab11570)Normal rabbit aorta, Lowicryl K4M thin section, stained with Monoclonal Anti-Chondroitin Sulfate, ab11570 and Goat Anti-Mouse IgM (µ-chain specific) 10 nm gold. Counterstain was uranyl acetate and Reynold's lead citrate. Magnification 44,600x. (el=Elastin, col=Collagen).
References
This product has been referenced in:
- Horsley H et al. A urine-dependent human urothelial organoid offers a potential alternative to rodent models of infection. Sci Rep 8:1238 (2018). Read more (PubMed: 29352171) »
- Bolinches-Amorós A et al. Generation of a human iPSC line from a patient with congenital glaucoma caused by mutation in CYP1B1 gene. Stem Cell Res 28:96-99 (2018). ICC/IF ; Human . Read more (PubMed: 29453128) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"