The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/10 - 1/50.
Dorsalizing factor. Key developmental protein that dorsalizes early vertebrate embryonic tissues by binding to ventralizing TGF-beta family bone morphogenetic proteins (BMPs) and sequestering them in latent complexes.
Expressed at the highest level in liver.
Belongs to the chordin family. Contains 4 CHRD domains. Contains 4 VWFC domains.
Cleaved by tolloid proteases; cleavage participates to dorsoventral patterning during early development.
Immunohistochemistry analysis of formalin-fixed, paraffin-embedded Human hepatocarcinoma tissue using 1/10-1/50 ab80146, in conjunction with a peroxidase-conjugated secondary antibody, followed by DAB staining.