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  1. Link

    chp1-antibody-ab18191.pdf

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Epigenetics and Nuclear Signaling RNAi Dicer
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Anti-Chp1 antibody (ab18191)

  • Datasheet
  • SDS
Reviews (1) Submit a question References (14)

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Western blot - Anti-Chp1 antibody (ab18191)
  • ChIP - Anti-Chp1 antibody (ab18191)
  • Western blot - Anti-Chp1 antibody (ab18191)

Key features and details

  • Rabbit polyclonal to Chp1
  • Suitable for: ChIP, WB
  • Reacts with: Schizosaccharomyces pombe
  • Isotype: IgG

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Overview

  • Product name

    Anti-Chp1 antibody
  • Description

    Rabbit polyclonal to Chp1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ChIP, WBmore details
  • Species reactivity

    Reacts with: Schizosaccharomyces pombe
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • This antibody gave a positive signal in S. pombe whole cell lysate.
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • RNAi
    • Dicer
    • Epigenetics and Nuclear Signaling
    • RNAi
    • RISC

Associated products

  • ChIP Related Products

    • Rabbit Anti-Mouse IgG H&L (ab46540)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab18191 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ChIP (1)
Use 2.5µg for 107 cells.
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 109 kDa (predicted molecular weight: 109 kDa).
Notes
ChIP
Use 2.5µg for 107 cells.
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 109 kDa (predicted molecular weight: 109 kDa).

Target

  • Relevance

    Chp1 a chromodomain protein associates with the S. pombe mating type locus and telomeres. Chp1 localization to heterochromatin depends on its chromodomain and the C-terminal domain of the protein. Chp1 and centromeric siRNAs are components of the RITS (RNA-induced initiation of transcriptional gene silencing) complex. The association of RITS with centromeres is linked to Dicer activity, which is required to cleave centromeric transcripts to produce short interfering RNAs that actively recruit components of heterochromatin to centromeres.
  • Cellular localization

    Nuclear
  • Database links

    • Entrez Gene: 2542239 Schizosaccharomyces pombe
    • SwissProt: Q10103 Schizosaccharomyces pombe
    • Alternative names

      • Chromo domain containing protein 1 antibody
      • Chromodomain protein Chp1 antibody

    Images

    • Western blot - Anti-Chp1 antibody (ab18191)
      Western blot - Anti-Chp1 antibody (ab18191)This image is courtesy of Danesh Moazed's Laboratory, Harvard, Boston.
      All lanes : Anti-Chp1 antibody (ab18191) at 1 µg/ml

      Lane 1 : S. pombe chp1Δ lysate
      Lane 2 : S. pombe wildtype lysate

      Predicted band size: 109 kDa
      Observed band size: 109 kDa
      Additional bands at: 102 kDa (possible degradation product)



      ab18191 specifically recognises Chp1 at 109 kDa in wildtype S. pombe lysate but not in chp1Δ lysate.
    • ChIP - Anti-Chp1 antibody (ab18191)
      ChIP - Anti-Chp1 antibody (ab18191)

      X-ChIP performed with ab18191 on Schizosaccharomyces pombe Cell lysate (whole cell). 5µl antibody were used per 500µl of chromatin/cell lysate according to standard S. pombe ChIP protocols. Protein-A Sepharose was used to recover the ChIPs. In the positive control the researcher specifically induced silencing of the ura4+ gene. For the negative control  the researcher performed multiplex PCR with primers amplifying ura4+ and an un-silenced gene. In addition, act1+ was amplified in a separate PCR reaction.  Chp1 only binds to ura4+ (and only if silencing is induced).

      This image is courtesy of an Abreview submitted by Marc B? on 9 February 2006

      See Abreview

    • Western blot - Anti-Chp1 antibody (ab18191)
      Western blot - Anti-Chp1 antibody (ab18191)
      Anti-Chp1 antibody (ab18191) at 1 µg/ml + S.pombe Whole Cell Lysate at 20 µg

      Secondary
      Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/10000 dilution

      Performed under reducing conditions.

      Predicted band size: 109 kDa
      Observed band size: 115 kDa why is the actual band size different from the predicted?
      Additional bands at: 75&42 kDa. We are unsure as to the identity of these extra bands.

    Protocols

    • ChIP protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (14)

    Publishing research using ab18191? Please let us know so that we can cite the reference in this datasheet.

    ab18191 has been referenced in 14 publications.

    • Gao Z  et al. FK506-binding protein 5 promotes the progression of papillary thyroid carcinoma. J Int Med Res 49:3000605211008325 (2021). PubMed: 33906532
    • Jih G  et al. Unique roles for histone H3K9me states in RNAi and heritable silencing of transcription. Nature 547:463-467 (2017). ChIP . PubMed: 28682306
    • Zocco M  et al. The Chp1 chromodomain binds the H3K9me tail and the nucleosome core to assemble heterochromatin. Cell Discov 2:16004 (2016). ChIP . PubMed: 27462451
    • Creamer KM  et al. The Mi-2 homolog Mit1 actively positions nucleosomes within heterochromatin to suppress transcription. Mol Cell Biol 34:2046-61 (2014). ChIP ; Schizosaccharomyces pombe . PubMed: 24662054
    • Shim YS  et al. Hrp3 controls nucleosome positioning to suppress non-coding transcription in eu- and heterochromatin. EMBO J 31:4375-87 (2012). PubMed: 22990236
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    ChIP abreview for Anti-Chp1 antibody - ChIP Grade

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    ChIP
    Sample
    Schizosaccharomyces pombe Cell lysate - whole cell
    Type
    Cross-linking (X-ChIP)
    Duration of cross-linking step: 0 second(s)
    Specification of the cross-linking agent: 3% Formaldehyde
    Detection step
    Semiquantitative PCR
    Positive control
    I specifically induce silencing of the ura4+ gene. I see enrichment for this gene in ChIPs performed with antibodies against H3K9me2, Ago1, Swi6 and, as I show here, Chp1. All these proteins are recruited to transcriptional silent loci as for example centromeric DNA repeats. The Chp1 antibody precipitates chromatin very well (see figure )-it gives me the strongest signal among all the antibodies I used so far.
    Negative control
    I perform multiplex PCR with primers amplifying ura4+ and an un-silenced gene. In addition, act1+ was amplified in a separate PCR reaction. As you can see, Chp1 only binds to ura4+ (and only if silencing is induced).
    Read More

    Dr. Marc B?

    Verified customer

    Submitted Feb 09 2006

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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