Anti-CHRAC-17 antibody (ab229060)
Key features and details
- Rabbit polyclonal to CHRAC-17
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-CHRAC-17 antibody
See all CHRAC-17 primary antibodies -
Description
Rabbit polyclonal to CHRAC-17 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Chicken, Cow, Xenopus laevis, Chimpanzee, Rhesus monkey -
Immunogen
Recombinant fragment within Human CHRAC-17 (internal sequence). The exact sequence is proprietary.
Database link: Q9NRF9 -
Positive control
- WB: HEK-293T whole cell lysate. IHC-P: Mouse muscle tissue; Cal27 xenograft tissue. ICC/IF: HeLa cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab229060 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/100 - 1/1000.
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WB |
1/500 - 1/3000. Predicted molecular weight: 16 kDa.
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ICC/IF |
1/100 - 1/1000.
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Notes |
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IHC-P
1/100 - 1/1000. |
WB
1/500 - 1/3000. Predicted molecular weight: 16 kDa. |
ICC/IF
1/100 - 1/1000. |
Target
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Function
Forms a complex with DNA polymerase epsilon subunit CHRAC1 and binds naked DNA, which is then incorporated into chromatin, aided by the nucleosome-remodeling activity of ISWI/SNF2H and ACF1. -
Tissue specificity
Expressed in all tissues tested, including, heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 417275 Chicken
- Entrez Gene: 510678 Cow
- Entrez Gene: 54107 Human
- Entrez Gene: 59001 Mouse
- Entrez Gene: 100362333 Rat
- Entrez Gene: 298098 Rat
- Omim: 607267 Human
- SwissProt: Q3SZN5 Cow
see all -
Alternative names
- Arsenic transactivated protein antibody
- Arsenic-transactivated protein antibody
- ASTP antibody
see all
Images
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Anti-CHRAC-17 antibody (ab229060) at 1/1000 dilution + HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 30 µg
Developed using the ECL technique.
Predicted band size: 16 kDa15% SDS-PAGE
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Paraffin-embedded mouse muscle tissue stained for CHRAC-17 with ab229060 at 1/1000 dilution in immunohistochemical analysis.
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Paraffin-embedded Cal27 xenograft tissue stained for CHRAC-17 with ab229060 at 1/500 dilution in immunohistochemical analysis.
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Paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for CHRAC-17 (green) using ab229060 at 1/200 dilution in ICC/IF.
Nuclear counterstain: Hoechst 33342 (blue).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab229060 has not yet been referenced specifically in any publications.