Key features and details
- Rabbit polyclonal to CHRAC-17
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-CHRAC-17 antibody
See all CHRAC-17 primary antibodies
DescriptionRabbit polyclonal to CHRAC-17
Tested applicationsSuitable for: IHC-P, WB, ICC/IFmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Chicken, Cow, Xenopus laevis, Chimpanzee, Rhesus monkey
Recombinant fragment within Human CHRAC-17 (internal sequence). The exact sequence is proprietary.
Database link: Q9NRF9
- WB: HEK-293T whole cell lysate. IHC-P: Mouse muscle tissue; Cal27 xenograft tissue. ICC/IF: HeLa cells.
Protein previously known as DNA Polymerase epsilon.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab229060 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/100 - 1/1000.|
|WB||1/500 - 1/3000. Predicted molecular weight: 16 kDa.|
|ICC/IF||1/100 - 1/1000.|
FunctionForms a complex with DNA polymerase epsilon subunit CHRAC1 and binds naked DNA, which is then incorporated into chromatin, aided by the nucleosome-remodeling activity of ISWI/SNF2H and ACF1.
Tissue specificityExpressed in all tissues tested, including, heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.
- Information by UniProt
- Arsenic transactivated protein antibody
- Arsenic-transactivated protein antibody
- ASTP antibody
Anti-CHRAC-17 antibody (ab229060) at 1/1000 dilution + HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 30 µg
Developed using the ECL technique.
Predicted band size: 16 kDa
Paraffin-embedded mouse muscle tissue stained for CHRAC-17 with ab229060 at 1/1000 dilution in immunohistochemical analysis.
Paraffin-embedded Cal27 xenograft tissue stained for CHRAC-17 with ab229060 at 1/500 dilution in immunohistochemical analysis.
Paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for CHRAC-17 (green) using ab229060 at 1/200 dilution in ICC/IF.
Nuclear counterstain: Hoechst 33342 (blue).
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab229060 has not yet been referenced specifically in any publications.