Overview

  • Product name

  • Description

    Rabbit polyclonal to CHRAC-17
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Chicken, Cow, Xenopus laevis, Chimpanzee, Rhesus monkey
  • Immunogen

    Recombinant fragment within Human CHRAC-17 (internal sequence). The exact sequence is proprietary.
    Database link: Q9NRF9

  • Positive control

    • WB: HEK-293T whole cell lysate. IHC-P: Mouse muscle tissue; Cal27 xenograft tissue. ICC/IF: HeLa cells.
  • General notes

    Protein previously known as DNA Polymerase epsilon.

Properties

Applications

Our Abpromise guarantee covers the use of ab229060 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/1000.
WB 1/500 - 1/3000. Predicted molecular weight: 16 kDa.
ICC/IF 1/100 - 1/1000.

Target

  • Function

    Forms a complex with DNA polymerase epsilon subunit CHRAC1 and binds naked DNA, which is then incorporated into chromatin, aided by the nucleosome-remodeling activity of ISWI/SNF2H and ACF1.
  • Tissue specificity

    Expressed in all tissues tested, including, heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • Arsenic transactivated protein antibody
    • Arsenic-transactivated protein antibody
    • ASTP antibody
    • CHARAC 17 antibody
    • CHARAC17 antibody
    • CHRAC 17 antibody
    • CHRAC-17 antibody
    • CHRAC17 antibody
    • Chromatin Accessibility Complex 17 antibody
    • Chromatin accessibility complex 17 kDa protein antibody
    • DNA Polymerase Epsilon p17 Subunit antibody
    • DNA polymerase epsilon subunit 3 antibody
    • DNA polymerase epsilon subunit p17 antibody
    • DNA polymerase II subunit 3 antibody
    • DPOE3_HUMAN antibody
    • Histone fold protein CHRAC17 antibody
    • HuCHRAC 17 antibody
    • HuCHRAC17 antibody
    • p17 antibody
    • p17 subunit antibody
    • POL E3 antibody
    • POLE 3 antibody
    • POLE3 antibody
    • Polymerase (DNA directed) epsilon 3 antibody
    • YBL 1 antibody
    • YBL1 antibody
    see all

Images

  • Anti-CHRAC-17 antibody (ab229060) at 1/1000 dilution + HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 30 µg

    Developed using the ECL technique.

    Predicted band size: 16 kDa



    15% SDS-PAGE

  • Paraffin-embedded mouse muscle tissue stained for CHRAC-17 with ab229060 at 1/1000 dilution in immunohistochemical analysis.

  • Paraffin-embedded Cal27 xenograft tissue stained for CHRAC-17 with ab229060 at 1/500 dilution in immunohistochemical analysis.

  • Paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for CHRAC-17 (green) using ab229060 at 1/200 dilution in ICC/IF.

    Nuclear counterstain: Hoechst 33342 (blue).

References

ab229060 has not yet been referenced specifically in any publications.

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There are currently no Customer reviews or Questions for ab229060.
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