Overview

  • Product name

  • Description

    Rabbit polyclonal to CHRAC-17
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Guinea pig, Cow, Chimpanzee, Rhesus monkey, Gorilla, Chinese hamster, Orangutan, Bat
  • Immunogen

    Synthetic peptide within Human CHRAC-17 aa 97-147. The exact sequence is proprietary. NP_059139.2

  • Positive control

    • WB: Lysate from HeLa or HEK-293T cells.
  • General notes

    Protein previously known as DNA Polymerase epsilon.

Properties

Applications

Our Abpromise guarantee covers the use of ab74308 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 16 kDa.
IP Use at 2-5 µg/mg of lysate.
IHC-P 1/1000 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Forms a complex with DNA polymerase epsilon subunit CHRAC1 and binds naked DNA, which is then incorporated into chromatin, aided by the nucleosome-remodeling activity of ISWI/SNF2H and ACF1.
  • Tissue specificity

    Expressed in all tissues tested, including, heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • Arsenic transactivated protein antibody
    • Arsenic-transactivated protein antibody
    • ASTP antibody
    • CHARAC 17 antibody
    • CHARAC17 antibody
    • CHRAC 17 antibody
    • CHRAC-17 antibody
    • CHRAC17 antibody
    • Chromatin Accessibility Complex 17 antibody
    • Chromatin accessibility complex 17 kDa protein antibody
    • DNA Polymerase Epsilon p17 Subunit antibody
    • DNA polymerase epsilon subunit 3 antibody
    • DNA polymerase epsilon subunit p17 antibody
    • DNA polymerase II subunit 3 antibody
    • DPOE3_HUMAN antibody
    • Histone fold protein CHRAC17 antibody
    • HuCHRAC 17 antibody
    • HuCHRAC17 antibody
    • p17 antibody
    • p17 subunit antibody
    • POL E3 antibody
    • POLE 3 antibody
    • POLE3 antibody
    • Polymerase (DNA directed) epsilon 3 antibody
    • YBL 1 antibody
    • YBL1 antibody
    see all

Images

  • All lanes : Anti-CHRAC-17 antibody (ab74308) at 0.4 µg/ml

    Lane 1 : Whole cell lysate from HeLa cells at 50 µg
    Lane 2 : Whole cell lysate from HeLa cells at 15 µg
    Lane 3 : Whole cell lysate from HeLa cells at 5 µg
    Lane 4 : Whole cell lysate from HEK-293T cells at 50 µg

    Predicted band size: 16 kDa
    Observed band size: 18 kDa
    why is the actual band size different from the predicted?

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma (left) and mouse teratoma (right) tissues labeling CHRAC-17 with ab74308 at 1/5000 (0.2 µg/ml). Detection: DAB.

  • Detection of human CHRAC-17 by immunoprecipitation of 1mg HeLa cells whole lysate, using ab74308 at 3µg/mg lysate (lane 1, 20% IP loaded) or a control IgG (lane 2, 20% IP loaded). The post-IP WB was performed using ab74308 at 1 µg/ml.

    Detection: chemiluminescence with an exposure time of 30 seconds.

References

This product has been referenced in:

  • Wu R  et al. H3K9me3 demethylase Kdm4d facilitates the formation of pre-initiative complex and regulates DNA replication. Nucleic Acids Res 45:169-180 (2017). WB . Read more (PubMed: 27679476) »
See 1 Publication for this product

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