Key features and details
- Rabbit polyclonal to CHRAC-17
- Suitable for: WB, IP, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-CHRAC-17 antibody
See all CHRAC-17 primary antibodies
DescriptionRabbit polyclonal to CHRAC-17
Tested applicationsSuitable for: WB, IP, IHC-Pmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Guinea pig, Cow, Chimpanzee, Rhesus monkey, Gorilla, Chinese hamster, Orangutan, Bat
Synthetic peptide within Human CHRAC-17 aa 97-147. The exact sequence is proprietary. NP_059139.2
- WB: Lysate from HeLa or HEK-293T cells.
Protein previously known as DNA Polymerase epsilon.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab74308 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 16 kDa.|
|IP||Use at 2-5 µg/mg of lysate.|
|IHC-P||1/1000 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionForms a complex with DNA polymerase epsilon subunit CHRAC1 and binds naked DNA, which is then incorporated into chromatin, aided by the nucleosome-remodeling activity of ISWI/SNF2H and ACF1.
Tissue specificityExpressed in all tissues tested, including, heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.
- Information by UniProt
- Arsenic transactivated protein antibody
- Arsenic-transactivated protein antibody
- ASTP antibody
All lanes : Anti-CHRAC-17 antibody (ab74308) at 0.4 µg/ml
Lane 1 : Whole cell lysate from HeLa cells at 50 µg
Lane 2 : Whole cell lysate from HeLa cells at 15 µg
Lane 3 : Whole cell lysate from HeLa cells at 5 µg
Lane 4 : Whole cell lysate from HEK-293T cells at 50 µg
Predicted band size: 16 kDa
Observed band size: 18 kDa why is the actual band size different from the predicted?
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma (left) and mouse teratoma (right) tissues labeling CHRAC-17 with ab74308 at 1/5000 (0.2 µg/ml). Detection: DAB.
Detection of human CHRAC-17 by immunoprecipitation of 1mg HeLa cells whole lysate, using ab74308 at 3µg/mg lysate (lane 1, 20% IP loaded) or a control IgG (lane 2, 20% IP loaded). The post-IP WB was performed using ab74308 at 1 µg/ml.
Detection: chemiluminescence with an exposure time of 30 seconds.
ab74308 has been referenced in 1 publication.
- Wu R et al. H3K9me3 demethylase Kdm4d facilitates the formation of pre-initiative complex and regulates DNA replication. Nucleic Acids Res 45:169-180 (2017). WB . PubMed: 27679476