Key features and details
- Rabbit polyclonal to CHRDL1
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat
- Isotype: IgG
Product nameAnti-CHRDL1 antibody
See all CHRDL1 primary antibodies
DescriptionRabbit polyclonal to CHRDL1
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Rat
Predicted to work with: Human, Rhesus monkey
Recombinant fragment within Human CHRDL1 (internal sequence). The exact sequence is proprietary.
Database link: Q9BU40
- WB: Rat brain tissue extract. IHC-P: Mouse kidney and hind brain tissue.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 79.99% PBS, 20% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab227473 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 51 kDa.|
|IHC-P||1/100 - 1/1000.|
FunctionAntagonizes the function of BMP4 by binding to it and preventing its interaction with receptors. Alters the fate commitment of neural stem cells from gliogenesis to neurogenesis. Contributes to neuronal differentiation of neural stem cells in the brain by preventing the adoption of a glial fate. May play a crucial role in dorsoventral axis formation. May play a role in embyonic bone formation (By similarity). May also play an important role in regulating retinal angiogenesis trough modulation of BMP4 actions in endothelial cells.
Tissue specificityExpressed in retinal pericytes.
Sequence similaritiesContains 3 VWFC domains.
- Information by UniProt
- CHL antibody
- chordin like 1 antibody
- Chordin-like protein 1 antibody
Anti-CHRDL1 antibody (ab227473) at 1/500 dilution + Rat brain tissue extract at 50 µg
Developed using the ECL technique.
Predicted band size: 51 kDa
Paraffin-embedded mouse hind brain tissue stained for CHRDL1 with ab227473 at 1/500 dilution in immunohistochemical analysis.
Paraffin-embedded mouse kidney tissue stained for CHRDL1 with ab227473 at 1/500 dilution in immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab227473 has not yet been referenced specifically in any publications.