Overview

  • Product name

    Anti-CHREBP antibody (HRP)
    See all CHREBP primary antibodies
  • Description

    Rabbit polyclonal to CHREBP (HRP)
  • Host species

    Rabbit
  • Conjugation

    HRP
  • Specificity

    A customer has observed some cross-reactivity with MLX-interacting protein (Q9HAP2). Although the exact sequence is proprietary, the immunogen for ab81958 shows 75% homology with this protein.
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human CHREBP aa 800-852 (C terminal). The exact sequence is proprietary.
    Database link: NP_116569.1

  • Positive control

    • Human hepatocyte lysate, human hepatocytes, liver nuclear extracts from well fed rats, and colon epithelium.

Properties

Applications

Our Abpromise guarantee covers the use of ab81958 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent dilution. Predicted molecular weight: 95 kDa.
IHC-P Use at an assay dependent dilution.
ICC/IF 1/100 - 1/500.

Target

  • Function

    Transcriptional repressor. Binds to the canonical and non-canonical E box sequences 5'-CACGTG-3'.
  • Tissue specificity

    Expressed in liver, heart, kidney, cerebellum and intestinal tissues.
  • Involvement in disease

    Note=WBSCR14 is located in the Williams-Beuren syndrome (WBS) critical region. WBS results from a hemizygous deletion of several genes on chromosome 7q11.23, thought to arise as a consequence of unequal crossing over between highly homologous low-copy repeat sequences flanking the deleted region. Haploinsufficiency of WBSCR14 may be the cause of certain cardiovascular and musculo-skeletal abnormalities observed in the disease.
  • Sequence similarities

    Contains 1 basic helix-loop-helix (bHLH) domain.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • carbohydrate response element binding protein antibody
    • bHLHd14 antibody
    • Carbohydrate responsive element binding protein antibody
    • Class D basic helix-loop-helix protein 14 antibody
    • MIO antibody
    • MLX interacting protein like antibody
    • Mlx interactor antibody
    • MLX-interacting protein-like antibody
    • MLXIPL antibody
    • MONDOB antibody
    • WBS14_HUMAN antibody
    • WBSCR 14 antibody
    • WBSCR14 antibody
    • Williams Beuren syndrome chromosome region 14 antibody
    • Williams Beuren syndrome chromosome region 14 protein antibody
    • Williams-Beuren syndrome chromosomal region 14 protein antibody
    • WS basic helix loop helix leucine zipper protein antibody
    • WS basic-helix-loop-helix leucine zipper protein antibody
    • WS bHLH antibody
    • WS-bHLH antibody
    see all

Images

  • anti CHREBP (unconjugated form of ab81958) + human hepatocyte lysate at 20 µg

    Predicted band size: 95 kDa
    Observed band size: 95 kDa



    5-10 second film exposure.
  • anti CHREBP (unconjugated form of ab81958), staining CHREBP in colon epithelium (40X) by immunohistochemistry.
  • anti CHREBP (unconjugated form of ab81958), at a 1/100 dilution, staining CHREBP in human hepatocyte by Immunofluorescence. Overlay staining of ChREBP (rhodamine red) and DAPI nuclear staining (blue).
  • All lanes : anti CHREBP (unconjugated form of ab81958), at 1/1000 dilution

    Lane 1 : liver nuclear extracts from well fed rats at 10 µg
    Lane 2 : liver nuclear extracts from well fed rats at 5 µg
    Lane 3 : liver nuclear extracts from well fed rats at 1 µg
    Lane 4 : liver nuclear extracts from well fed rats at 0.5 µg

    Predicted band size: 95 kDa
    Observed band size: 95 kDa



    7% SDS gel.

References

This product has been referenced in:

  • Sillam-Dussès D  et al. The role of the glucose-sensing transcription factor carbohydrate-responsive element-binding protein pathway in termite queen fertility. Open Biol 6:N/A (2016). WB, IHC . Read more (PubMed: 27249798) »
  • Sujishi T  et al. Sitagliptin can inhibit the development of hepatic steatosis in high-fructose diet-fed ob/ob mice. J Clin Biochem Nutr 57:244-53 (2015). WB . Read more (PubMed: 26566312) »
See all 3 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Western blot
Sample
Mouse Tissue lysate - whole (liver and fat tissue)
Gel Running Conditions
Reduced Denaturing (10%)
Loading amount
50 µg
Specification
liver and fat tissue
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

Abcam user community

Verified customer

Submitted Feb 17 2016

Question
Answer


Pour visualiser l’HRP il faut ajouter un substrat qui, lorsqu'il est oxydé par l'HRP, change de couleur ou produit de la lumière qui peut être détecté.
De nombreux substrats pour HRP peuvent être utilisés :
HRP catalyse la conversion des substrats chromogènes (par exemple, TMB, DAB, ABTS) en produits colorés.
HRP produit de la lumière quand il agit sur des substrats chimioluminescent, par exemple ECL.
Dans notre catalogue nous vendons plusieurs de ces substrats. Par exemple :
Pour l’immunohistochimie :
DAB Substrate Kit (ab64238)
DAB substrate kit (ab94665)
Pour le western blot :
ECL Western Blotting Substrate Kit - 50 Tests (ab65623)
Chemiluminescent HRP Substrate (membrane) (ab5801)
Optiblot ECL Ultra Detect Kit (1.2pg-2ng) (ab133409)
Optiblot ECL Detect Kit (23pg-187ng) (ab133406)
Optiblot ECL Max Detect Kit (4.6pg-4.7ng) (ab133408)

Read More

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