Overview

  • Product name
  • Description
    Rabbit polyclonal to CHRNA10
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide within Human CHRNA10 aa 186-214 (internal sequence) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    Database link: Q9GZZ6

  • Positive control
    • NCI-H292, MDA-MB453 and ZR-75-1 cell line lysates; Human tonsil tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab170602 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Predicted molecular weight: 50 kDa.Can be blocked with CHRNA10 peptide (ab211875).
IHC-P 1/10 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    Ionotropic receptor with a probable role in the modulation of auditory stimuli. Agonist binding may induce an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane. The channel is permeable to a range of divalent cations including calcium, the influx of which may activate a potassium current which hyperpolarizes the cell membrane. In the ear, this may lead to a reduction in basilar membrane motion, altering the activity of auditory nerve fibers and reducing the range of dynamic hearing. This may protect against acoustic trauma.
  • Tissue specificity
    Expressed in inner-ear tissue, tonsil, immortalized B-cells, cultured T-cells and peripheral blood lymphocytes.
  • Sequence similarities
    Belongs to the ligand-gated ion channel (TC 1.A.9) family. Acetylcholine receptor (TC 1.A.9.1) subfamily. Alpha-10/CHRNA10 sub-subfamily.
  • Cellular localization
    Cell junction > synapse > postsynaptic cell membrane. Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Acetylcholine receptor, neuronal nicotinic, alpha-10 subunit antibody
    • ACH10_HUMAN antibody
    • Alpha 10 nAChR antibody
    • Cholinergic receptor nicotinic alpha 10 antibody
    • Cholinergic receptor, neuronal nicotinic, alpha polypeptide 10 antibody
    • Cholinergic receptor, nicotinic, alpha polypeptide 10 antibody
    • CHRNA 10 antibody
    • CHRNA10 antibody
    • NACHR alpha 10 antibody
    • NACHR alpha-10 antibody
    • NACHRA10 antibody
    • Neuronal acetylcholine receptor protein subunit alpha 10 antibody
    • Neuronal acetylcholine receptor subunit alpha-10 antibody
    • Nicotinic acetylcholine receptor subunit alpha 10 antibody
    • Nicotinic acetylcholine receptor subunit alpha-10 antibody
    • OTTHUMP00000013620 antibody
    see all

Images

  • All lanes : Anti-CHRNA10 antibody (ab170602) at 1/100 dilution

    Lane 1 : NCI-H292 cell line lysate
    Lane 2 : MDA-MB453 cell line lysate
    Lane 3 : ZR-75-1 cell line lysate

    Lysates/proteins at 35 µg per lane.

    Predicted band size: 50 kDa

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human tonsil tissue labeling CHRNA10 with ab170602 at 1/10 dilution, followed by peroxidase conjugation of the secondary antibody and DAB staining.

References

ab170602 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Brain microvascular endothelial cells)
Permeabilization
Yes - 0.1% Triton-x100
Specification
Brain microvascular endothelial cells
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: RT°C
Fixative
Paraformaldehyde
Username

Yi-An Yang

Verified customer

Submitted Jan 05 2016

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Brain, cerebrum cortex)
Permeabilization
Yes - 0.05% Tween 20
Specification
Brain, cerebrum cortex
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: RT°C
Fixative
Paraformaldehyde
Username

Yi-An Yang

Verified customer

Submitted Jan 05 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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