Overview

  • Product name

    Anti-CHRNA2 antibody
  • Description

    Rabbit polyclonal to CHRNA2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to Human CHRNA2 aa 27-264.
    Database link: Q15822

  • Positive control

    • WB: HL60 whole cell lysate. IHC-P: Human tonsil and spleen tissues.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.3
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 50% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab230287 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Detects a band of approximately 60 kDa (predicted molecular weight: 60,58 kDa).
IHC-P 1/20 - 1/200.

Target

  • Function

    After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane.
  • Involvement in disease

    Defects in CHRNA2 are the cause of nocturnal frontal lobe epilepsy type 4 (ENFL4) [MIM:610353]. ENFL4 is an autosomal dominant epilepsy characterized by nocturnal seizures associated with fear sensation, tongue movements, and nocturnal wandering, closely resembling nightmares and sleep walking.
  • Sequence similarities

    Belongs to the ligand-gated ion channel (TC 1.A.9) family. Acetylcholine receptor (TC 1.A.9.1) subfamily. Alpha-2/CHRNA2 sub-subfamily.
  • Cellular localization

    Cell junction > synapse > postsynaptic cell membrane. Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • ACHA2_HUMAN antibody
    • AChR antibody
    • Cholinergic receptor nicotinic alpha 2 antibody
    • Chrna2 antibody
    • Neuronal acetylcholine receptor protein subunit alpha 2 antibody
    • Neuronal acetylcholine receptor subunit alpha-2 antibody
    • Nicotinic Acetylcholine Receptor alpha 2 antibody
    see all

Images

  • Paraffin-embedded human spleen tissue stained for CHRNA2 using ab230287 at 1/100 dilution in immunohistochemical analysis.

  • Paraffin-embedded human tonsil tissue stained for CHRNA2 using ab230287 at 1/100 dilution in immunohistochemical analysis.

  • Anti-CHRNA2 antibody (ab230287) at 1/1000 dilution + HL-60 (human promyelocytic leukemia cell line) whole cell lysate

    Secondary
    Goat polyclonal to rabbit IgG at 1/10000 dilution

    Predicted band size: 60,58 kDa
    Observed band size: 60 kDa
    why is the actual band size different from the predicted?

References

ab230287 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab230287.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up