Key features and details
- Rabbit polyclonal to CHRNA2
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-CHRNA2 antibody
DescriptionRabbit polyclonal to CHRNA2
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Recombinant fragment corresponding to Human CHRNA2 aa 27-264.
Database link: Q15822
- WB: HL60 whole cell lysate. IHC-P: Human tonsil and spleen tissues.
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We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab230287 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/5000. Detects a band of approximately 60 kDa (predicted molecular weight: 60,58 kDa).|
|IHC-P||1/20 - 1/200.|
FunctionAfter binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane.
Involvement in diseaseDefects in CHRNA2 are the cause of nocturnal frontal lobe epilepsy type 4 (ENFL4) [MIM:610353]. ENFL4 is an autosomal dominant epilepsy characterized by nocturnal seizures associated with fear sensation, tongue movements, and nocturnal wandering, closely resembling nightmares and sleep walking.
Sequence similaritiesBelongs to the ligand-gated ion channel (TC 1.A.9) family. Acetylcholine receptor (TC 1.A.9.1) subfamily. Alpha-2/CHRNA2 sub-subfamily.
Cellular localizationCell junction > synapse > postsynaptic cell membrane. Cell membrane.
- Information by UniProt
- ACHA2_HUMAN antibody
- AChR antibody
- Cholinergic receptor nicotinic alpha 2 antibody
Paraffin-embedded human spleen tissue stained for CHRNA2 using ab230287 at 1/100 dilution in immunohistochemical analysis.
Paraffin-embedded human tonsil tissue stained for CHRNA2 using ab230287 at 1/100 dilution in immunohistochemical analysis.
Anti-CHRNA2 antibody (ab230287) at 1/1000 dilution + HL-60 (human promyelocytic leukemia cell line) whole cell lysate
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 60,58 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab230287 has not yet been referenced specifically in any publications.