Key features and details
- Mouse monoclonal [5B6G8] to CHRNA6
- Suitable for: ICC/IF, Flow Cyt, WB, IHC-P
- Reacts with: Human
- Isotype: IgG1
Product nameAnti-CHRNA6 antibody [5B6G8]
See all CHRNA6 primary antibodies
DescriptionMouse monoclonal [5B6G8] to CHRNA6
Tested applicationsSuitable for: ICC/IF, Flow Cyt, WB, IHC-Pmore details
Species reactivityReacts with: Human
Recombinant fragment corresponding to Human CHRNA6 aa 26-239. (Expressed in E.coli).
Database link: Q15825
- WB: Recombinant human CHRNA6 (aa 26-239) protein; CHRNA6 (aa 26-239)-hIgGFc transfected HEK-293 cell lysate. IHC-P: Human bladder cancer tissue. ICC/IF: HeLa cells. Flow Cyt: SH-SY5Y and SK-N-SH cells.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.05% Sodium azide
Concentration information loading...
PurityProtein G purified
Purification notesPurified from tissue culture supernatant.
Our Abpromise guarantee covers the use of ab233721 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/100 - 1/500.|
|Flow Cyt||1/200 - 1/400.|
|WB||1/500 - 1/2000. Predicted molecular weight: 57 kDa.|
|IHC-P||1/200 - 1/1000.|
RelevanceNicotinic acetylcholine receptors (nAChRs, AChRs) are protein complexes made up of protein subunits that coassemble to form an ion channel. This ion channel is gated through the binding of the neurotransmitter acetylcholine (ACh) to its ligand-binding site on the AChR. After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane. CHRNA6 (cholinergic nicotinic receptor alpha polypeptide 6) is a neuronal acetylcholine receptor protein.
Cellular localizationCell junction, synapse, postsynaptic cell membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein.
- AChR-alpha6 antibody
- Acra6 antibody
- Cholinergic receptor nicotinic alpha 6 antibody
Anti-CHRNA6 antibody [5B6G8] (ab233721) + Recombinant human CHRNA6 (aa 26-239) protein
Predicted band size: 57 kDa
Expected MW is 51.2 kDa.
All lanes : Anti-CHRNA6 antibody [5B6G8] (ab233721) at 1/500 dilution
Lane 1 : HEK-293 (human epithelial cell line from embryonic kidney) cell lysate
Lane 2 : CHRNA6 (aa 26-239)-hIgGFc transfected HEK HEK-293 cell lysate
Predicted band size: 57 kDa
HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for CHRNA6 (green) using ab233721 at 1/100 dilution in ICC/IF.
Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Flow cytometric analysis of SH-SY5Y (human neuroblastoma cell line from bone marrow) cell line labeling CHRNA6 with ab233721 at 1/200 dilution (green) compared with a negative control (red).
Flow cytometric analysis of SK-N-SH (human neuroblastoma cell line) cell line labeling CHRNA6 with ab233721 at 1/200 dilution (green) compared with a negative control (red).
Paraffin-embedded human bladder cancer tissue stained for CHRNA6 using ab233721 at 1/200 dilution in immunohistochemical analysis. DAB staining.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab233721 has not yet been referenced specifically in any publications.