Overview

  • Product name

    Anti-CHRNA6 antibody [5B6G8]
    See all CHRNA6 primary antibodies
  • Description

    Mouse monoclonal [5B6G8] to CHRNA6
  • Host species

    Mouse
  • Tested applications

    Suitable for: ICC/IF, Flow Cyt, WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to Human CHRNA6 aa 26-239. (Expressed in E.coli).
    Database link: Q15825

  • Positive control

    • WB: Recombinant human CHRNA6 (aa 26-239) protein; CHRNA6 (aa 26-239)-hIgGFc transfected HEK-293 cell lysate. IHC-P: Human bladder cancer tissue. ICC/IF: HeLa cells. Flow Cyt: SH-SY5Y and SK-N-SH cells.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.05% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Protein G purified
  • Purification notes

    Purified from tissue culture supernatant.
  • Clonality

    Monoclonal
  • Clone number

    5B6G8
  • Isotype

    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab233721 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100 - 1/500.
Flow Cyt 1/200 - 1/400.
WB 1/500 - 1/2000. Predicted molecular weight: 57 kDa.
IHC-P 1/200 - 1/1000.

Target

  • Relevance

    Nicotinic acetylcholine receptors (nAChRs, AChRs) are protein complexes made up of protein subunits that coassemble to form an ion channel. This ion channel is gated through the binding of the neurotransmitter acetylcholine (ACh) to its ligand-binding site on the AChR. After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane. CHRNA6 (cholinergic nicotinic receptor alpha polypeptide 6) is a neuronal acetylcholine receptor protein.
  • Cellular localization

    Cell junction, synapse, postsynaptic cell membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein.
  • Database links

  • Alternative names

    • AChR-alpha6 antibody
    • Acra6 antibody
    • Cholinergic receptor nicotinic alpha 6 antibody
    • Cholinergic receptor nicotinic alpha polypeptide 6 antibody
    • CHRNA 6 antibody
    • nAChR alpha 6 antibody
    • NACHRA6 antibody
    • Neuronal acetylcholine receptor subunit alpha 6 antibody
    • Nicotinic acetylcholine receptor alpha6 antibody
    see all

Images

  • Anti-CHRNA6 antibody [5B6G8] (ab233721) + Recombinant human CHRNA6 (aa 26-239) protein

    Predicted band size: 57 kDa



    Expected MW is 51.2 kDa.

  • All lanes : Anti-CHRNA6 antibody [5B6G8] (ab233721) at 1/500 dilution

    Lane 1 : HEK-293 (human epithelial cell line from embryonic kidney) cell lysate
    Lane 2 : CHRNA6 (aa 26-239)-hIgGFc transfected HEK HEK-293 cell lysate

    Predicted band size: 57 kDa

  • HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for CHRNA6 (green) using ab233721 at 1/100 dilution in ICC/IF.

    Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

  • Flow cytometric analysis of SH-SY5Y (human neuroblastoma cell line from bone marrow) cell line labeling CHRNA6 with ab233721 at 1/200 dilution (green) compared with a negative control (red).

  • Flow cytometric analysis of SK-N-SH (human neuroblastoma cell line) cell line labeling CHRNA6 with ab233721 at 1/200 dilution (green) compared with a negative control (red).

  • Paraffin-embedded human bladder cancer tissue stained for CHRNA6 using ab233721 at 1/200 dilution in immunohistochemical analysis. DAB staining.

References

ab233721 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab233721.
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