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Neuroscience Neurotransmission Receptors / Channels Ligand-Gated Ion Channels nAch Receptors
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Anti-CHRNA9 antibody (ab177119)

  • Datasheet
  • SDS
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Abpromise

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Find out more.

Western blot - Anti-CHRNA9 antibody (ab177119)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CHRNA9 antibody (ab177119)

Key features and details

  • Rabbit polyclonal to CHRNA9
  • Suitable for: IHC-P, WB
  • Reacts with: Human
  • Isotype: IgG

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Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

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Overview

  • Product name

    Anti-CHRNA9 antibody
  • Description

    Rabbit polyclonal to CHRNA9
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide corresponding to Human CHRNA9.
    Database link: Q9UGM1

  • Positive control

    • Jurkat and HeLa lysates; Human brain tissue.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Lyophilized:Reconstitute in 200ul sterile H2O.
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 1% BSA, 98% PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Neurotransmission
    • Receptors / Channels
    • Ligand-Gated Ion Channels
    • nAch Receptors

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • HeLa whole cell lysate (ab150035)
    • HeLa whole cell lysate (ab29545)
    • Jurkat whole cell lysate (ab30128)
    • Jurkat whole cell lysate (ab7899)

Applications

Our Abpromise guarantee covers the use of ab177119 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/500.
WB 1/500 - 1/1000. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa).

Target

  • Relevance

    CHRNA9 is a member of the ligand gated ionic channel family and nicotinic acetylcholine receptor superfamily. CHRNA9 forms homo- or hetero-oligomeric divalent cation channels. It is involved in cochlea hair cell development and is also expressed in the outer hair cells (OHCs) of the adult cochlea. The protein is additionally expressed in keratinocytes, the pituitary gland, B-cells and T-cells.
  • Cellular localization

    Membrane; multi pass membrane protein
  • Database links

    • Entrez Gene: 55584 Human
    • Entrez Gene: 231252 Mouse
    • Entrez Gene: 65024 Rat
    • Omim: 605116 Human
    • SwissProt: Q9UGM1 Human
    • SwissProt: P43144 Rat
    • Unigene: 272278 Human
    • Unigene: 140015 Mouse
    • Unigene: 54463 Rat
    see all
  • Alternative names

    • Cholinergic receptor nicotinic alpha 9 antibody
    • Cholinergic receptor nicotinic alpha polypeptide 9 antibody
    • HSA243342 antibody
    • MGC142109 antibody
    • MGC142135 antibody
    • NACHR alpha 9 antibody
    • NACHRA9 antibody
    • Neuronal acetylcholine receptor protein subunit alpha 9 precursor antibody
    see all

Images

  • Western blot - Anti-CHRNA9 antibody (ab177119)
    Western blot - Anti-CHRNA9 antibody (ab177119)
    All lanes : Anti-CHRNA9 antibody (ab177119) at 1/500 dilution

    Lane 1 : Jurkat lysate
    Lane 2 : HeLa lysate

    Predicted band size: 55 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CHRNA9 antibody (ab177119)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CHRNA9 antibody (ab177119)

    Immunohistochemical analysis of paraffin embedded Human brain tissue labeling CHRNA9 with ab177119 at 1/100.

Protocols

  • Western blot protocols
  • Immunohistochemistry protocols

Click here to view the general protocols

Datasheets and documents

    • Datasheet
    • SDS
  • References (0)

    Publishing research using ab177119? Please let us know so that we can cite the reference in this datasheet.

    ab177119 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Show All Reviews Q&A
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    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CHRNA9 antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Pig Tissue sections (brain)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Tris/EDTA
    Permeabilization
    No
    Specification
    brain
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 24°C
    Fixative
    10% formalin
    Read More

    Abcam user community

    Verified customer

    Submitted Jun 12 2015

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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