Overview

  • Product name
    Anti-CHRNA9 antibody
    See all CHRNA9 primary antibodies
  • Description
    Rabbit polyclonal to CHRNA9
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide corresponding to Human CHRNA9.
    Database link: Q9UGM1

  • Positive control
    • Jurkat and HeLa lysates; Human brain tissue.

Properties

  • Form
    Lyophilised:Reconstitute in 200ul sterile H2O.
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.2
    Preservative: 0.02% Sodium azide
    Constituents: 1% BSA, 98% PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab177119 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/500.
WB 1/500 - 1/1000. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa).

Target

  • Relevance
    CHRNA9 is a member of the ligand gated ionic channel family and nicotinic acetylcholine receptor superfamily. CHRNA9 forms homo- or hetero-oligomeric divalent cation channels. It is involved in cochlea hair cell development and is also expressed in the outer hair cells (OHCs) of the adult cochlea. The protein is additionally expressed in keratinocytes, the pituitary gland, B-cells and T-cells.
  • Cellular localization
    Membrane; multi pass membrane protein
  • Database links
    see all
  • Alternative names
    • Cholinergic receptor nicotinic alpha 9 antibody
    • Cholinergic receptor nicotinic alpha polypeptide 9 antibody
    • HSA243342 antibody
    • MGC142109 antibody
    • MGC142135 antibody
    • NACHR alpha 9 antibody
    • NACHRA9 antibody
    • Neuronal acetylcholine receptor protein subunit alpha 9 precursor antibody
    see all

Images

  • Western blot - Anti-CHRNA9 antibody (ab177119)
    Western blot - Anti-CHRNA9 antibody (ab177119)
    All lanes : Anti-CHRNA9 antibody (ab177119) at 1/500 dilution

    Lane 1 : Jurkat lysate
    Lane 2 : HeLa lysate

    Predicted band size: 55 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CHRNA9 antibody (ab177119)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CHRNA9 antibody (ab177119)

    Immunohistochemical analysis of paraffin embedded Human brain tissue labeling CHRNA9 with ab177119 at 1/100.

References

ab177119 has not yet been referenced specifically in any publications.

Publishing research using ab177119? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CHRNA9 antibody

 Good
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Pig Tissue sections (brain)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris/EDTA
Permeabilization
No
Specification
brain
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 24°C
Fixative
10% formalin
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Submitted Jun 12 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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