Anti-CHRNB4 antibody [7F5B2] (ab233735)
Key features and details
- Mouse monoclonal [7F5B2] to CHRNB4
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-CHRNB4 antibody [7F5B2]
See all CHRNB4 primary antibodies -
Description
Mouse monoclonal [7F5B2] to CHRNB4 -
Host species
Mouse -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human CHRNB4 aa 22-236. Expressed in E.coli.
Database link: P30926 -
Positive control
- Recombinant human CHRNB4; CHRNB4 (AA: extra 22-236)-hIgGFc transfected HEK-293 cell lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
7F5B2 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab233735 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/500 - 1/2000.
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Notes |
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WB
1/500 - 1/2000. |
Target
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Function
After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane. -
Sequence similarities
Belongs to the ligand-gated ion channel (TC 1.A.9) family. Acetylcholine receptor (TC 1.A.9.1) subfamily. Beta-4/CHRNB4 sub-subfamily. -
Cellular localization
Cell junction > synapse > postsynaptic cell membrane. Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 1143 Human
- Omim: 118509 Human
- SwissProt: P30926 Human
- Unigene: 624178 Human
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Alternative names
- acetylcholine receptor, nicotinic, beta 4 (neuronal) antibody
- ACHB4_HUMAN antibody
- AChR antibody
see all
Images
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All lanes : Anti-CHRNB4 antibody [7F5B2] (ab233735) at 1/500 dilution
Lane 1 : HEK-293 (human epithelial cell line from embryonic kidney) cell lysate.
Lane 2 : CHRNB4 (AA: extra 22-236)-hIgGFc transfected HEK-293 cell lysate. -
Anti-CHRNB4 antibody [7F5B2] (ab233735) at 1/500 dilution + Recombinant human CHRNB4
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (0)
ab233735 has not yet been referenced specifically in any publications.