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What are the negative and positive control primers? Also, what is the concentration of DNA i hope to get at the end?
Asked on Jan 28 2015
The positive primers target human GAPDH housekeeping gene that is expressed in most cell lines with an accessible chromatin structure:
Negative primers target human HBB gene that is silenced in most cells with an inaccessible chromatin structure.
DNA yield is dependent on the amount of cells used for the reaction.
Kevin HansonAbcam Scientific Support
Answered on Jan 28 2015