Chromatin Extraction Kit (ab117152)
Key features and details
- Assay time: 1 hr
- Sample type: Adherent cells, Suspension cells, Tissue
Overview
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Product name
Chromatin Extraction Kit
See all Chromatin Extraction kits -
Sample type
Tissue, Adherent cells, Suspension cells -
Assay time
1h 00m -
Product overview
Chromatin Extraction Kit (ab117152) is a complete set of optimized buffers and reagents for isolating chromatin or DNA-protein complex from mammalian cells or tissues in a simple and rapid format. Chromatin prepared by this kit can be used in a variety of chromatin immunoprecipitation methods. The isolated chromatin can also be used in other chromatin-related applications such as in vitro protein-DNA binding assays and nuclear enzyme assays.
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 100 tests 1000X Protease Inhibitor Cocktail 1 x 110µl 10X Lysis Buffer 1 x 11ml Chromatin Buffer 1 x 11ml Extraction Buffer 1 x 11ml -
Research areas
Images
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ChIP analysis of RNA polymerase II enriched in GAPDH and MLH1 promoters with chromatin extract prepared from formaldehyde fixed colon cancer cells (2x105) using ab117152.
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Chromatin was extracted from 3x106 mouse neuroblastoma cells, fixed for 10min at RT in 0.1% formaldehyde. Cells were sonicated before last centrifugation, and following centrifugation, cells were treated with proteinase K & RNAse A. Chromatin yield was 4 µg chromatin/106 cells. Lane 1: 1kb DNA ladder Lane 2: 300 - 650bp DNA fragments
Image courtesy of anonymous Abreview
Datasheets and documents
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SDS download
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Datasheet download
References (24)
ab117152 has been referenced in 24 publications.
- Shen JZ et al. FBXO44 promotes DNA replication-coupled repetitive element silencing in cancer cells. Cell 184:352-369.e23 (2021). PubMed: 33357448
- Wang J et al. RAD52 Adjusts Repair of Single-Strand Breaks via Reducing DNA-Damage-Promoted XRCC1/LIG3a Co-localization. Cell Rep 34:108625 (2021). PubMed: 33440161
- Su W et al. Overexpressed WDR3 induces the activation of Hippo pathway by interacting with GATA4 in pancreatic cancer. J Exp Clin Cancer Res 40:88 (2021). PubMed: 33648545
- Guo F et al. NR5A2 transcriptional activation by BRD4 promotes pancreatic cancer progression by upregulating GDF15. Cell Death Discov 7:78 (2021). PubMed: 33850096
- Hang Q et al. Non-canonical function of DGCR8 in DNA double-strand break repair signaling and tumor radioresistance. Nat Commun 12:4033 (2021). PubMed: 34188037