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Chromatin Extraction Kit (ab117152)

Submit a review Q&A (22)References (24)
Functional Studies - Chromatin Extraction kit (ab117152)
  • Chromatin Extraction Kit (ab117152)

Key features and details

  • Assay time: 1 hr
  • Sample type: Adherent cells, Suspension cells, Tissue

Overview

  • Product name

    Chromatin Extraction Kit
    See all Chromatin Extraction kits

  • Sample type

    Tissue, Adherent cells, Suspension cells

  • Assay time

    1h 00m

  • Product overview

    Chromatin Extraction Kit (ab117152) is a complete set of optimized buffers and reagents for isolating chromatin or DNA-protein complex from mammalian cells or tissues in a simple and rapid format. Chromatin prepared by this kit can be used in a variety of chromatin immunoprecipitation methods. The isolated chromatin can also be used in other chromatin-related applications such as in vitro protein-DNA binding assays and nuclear enzyme assays.

     

Properties

Images

  • Functional Studies - Chromatin Extraction kit (ab117152)
    Functional Studies - Chromatin Extraction kit (ab117152)
    ChIP analysis of RNA polymerase II enriched in GAPDH and MLH1 promoters with chromatin extract prepared from formaldehyde fixed colon cancer cells (2x105) using ab117152.
  • Chromatin Extraction Kit (ab117152)
    Chromatin Extraction Kit (ab117152)
    Chromatin was extracted from 3x106 mouse neuroblastoma cells, fixed for 10min at RT in 0.1% formaldehyde. Cells were sonicated before last centrifugation, and following centrifugation, cells were treated with proteinase K & RNAse A. Chromatin yield was 4 µg chromatin/106 cells. Lane 1: 1kb DNA ladder Lane 2: 300 - 650bp DNA fragments
    Image courtesy of anonymous Abreview

References (24)

Publishing research using ab117152? Please let us know so that we can cite the reference in this datasheet.

ab117152 has been referenced in 24 publications.

  • Shen JZ  et al. FBXO44 promotes DNA replication-coupled repetitive element silencing in cancer cells. Cell 184:352-369.e23 (2021). PubMed: 33357448
  • Wang J  et al. RAD52 Adjusts Repair of Single-Strand Breaks via Reducing DNA-Damage-Promoted XRCC1/LIG3a Co-localization. Cell Rep 34:108625 (2021). PubMed: 33440161
  • Su W  et al. Overexpressed WDR3 induces the activation of Hippo pathway by interacting with GATA4 in pancreatic cancer. J Exp Clin Cancer Res 40:88 (2021). PubMed: 33648545
  • Guo F  et al. NR5A2 transcriptional activation by BRD4 promotes pancreatic cancer progression by upregulating GDF15. Cell Death Discov 7:78 (2021). PubMed: 33850096
  • Hang Q  et al. Non-canonical function of DGCR8 in DNA double-strand break repair signaling and tumor radioresistance. Nat Commun 12:4033 (2021). PubMed: 34188037
View all Publications for this product

Customer reviews and Q&As

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21-22 of 22 Abreviews or Q&A

Question

What is the procedure for preparing tissue from mouse brain? For example, do you perfuse the mouse with 1.5% PFA and then microdissect the region of interest? Is there a minimal volume of tissue needed for detection?

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Answer

Thank you for your inquiry.

You can find our ChIP tissue protocol here, which is optimized for 30 mg of liver tissue for each ChIP/antibody.

https://www.abcam.com/index.html?pageconfig=resource&rid=11803

For brain, it's best to use unfixed tissue. Here are some helpful references:

http://www.biomedcentral.com/1471-2202/9/42/

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2586869/

We do supply chromatin extraction kits that have been optimized for fresh or frozen tissues.

https://www.abcam.com/EpiSeeker-Chromatin-Extraction-Kit-ab117152.html

Also, we have modification-specific ChIP kits optimized for tissues. One example would be ab117150.

https://www.abcam.com/EpiSeeker-ChIP-Kit-Histone-H3-acetyl-Tissue-ab117150.html

https://www.abcam.com/index.html?pageconfig=resource&rid=14469&source=pagetrap&viapagetrap=episeeker

I hope this information helps. Please contact us with any other questions.

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Question

Many thanks for your mail and very useful information. I will be grateful if you can give me the discount code for ab193 antibody. We are working with brine shrimp (Artemia) and once I have the result I can send it to you. I am still looking for a good histone extraction method. I dont know which will suit the best for the antibody.I dont know if I can use any histone extraction kit. I will be grateful for your help. Thank you in advance Kind regards

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Answer

Thank you for your kind response. As I have explained in my previous e-mail any human lysate will probably have high levels of histone acetylation. However, it may be useful to treat the cells to maximize the level of acetylation. We have a very informative landing page for Epigenetics & Nuclear studies, please take a look at it: https://www.abcam.com/index.html?pageconfig=resource&rid=13907 Nuclear extraction kits: - ab113474 - ab113477 - ab117152 Histon extraction kit: - ab113476 DISCOUNT CODE: 100%ABR-XXXXXX Expiration date: XXXXXXX I am very pleased to hear you would like to accept our offer and test ab193 in brine shrimp (Artemia). This code will give you 1 free PRIMARY ANTIBODY before the expiration date. To redeem this offer, please submit an Abreview for brine shrimp (Artemia) and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews. Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code. Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research. The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

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21-22 of 22 Abreviews or Q&A

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