Recombinant
RabMAb

Anti-Chromodomain helicase DNA binding protein 5 antibody [EPR6053] (ab124736)

Overview

  • Product name
    Anti-Chromodomain helicase DNA binding protein 5 antibody [EPR6053]
    See all Chromodomain helicase DNA binding protein 5 primary antibodies
  • Description
    Rabbit monoclonal [EPR6053] to Chromodomain helicase DNA binding protein 5
  • Host species
    Rabbit
  • Specificity
    This antibody is predicted to cross-react with Chromodomain helicase DNA binding protein 3.
  • Tested applications
    Suitable for: WB, ICC/IFmore details
    Unsuitable for: Flow Cyt,IHC-P or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human Chromodomain helicase DNA binding protein 5 aa 1800-1900. The exact sequence is proprietary.

  • Positive control
    • Human adrenal gland and SH-SY5Y cell lysates; SH-SY5Y cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Dissociation constant (KD)
    KD = 3.10 x 10 -12 M
    Learn more about KD
  • Storage buffer
    pH: 7.20
    Preservative: 0.05% Sodium azide
    Constituents: 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    EPR6053
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab124736 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/50000 - 1/200000. Detects a band of approximately 260 kDa (predicted molecular weight: 223 kDa).
ICC/IF 1/250 - 1/500.
  • Application notes
    Is unsuitable for Flow Cyt,IHC-P or IP.
  • Target

    • Function
      May play a role in the development of the nervous system and the pathogenesis of neural tumors.
    • Tissue specificity
      Preferentially expressed in total brain, fetal brain, and cerebellum. It is also moderately expressed in the adrenal gland.
    • Sequence similarities
      Belongs to the SNF2/RAD54 helicase family.
      Contains 2 chromo domains.
      Contains 1 helicase ATP-binding domain.
      Contains 1 helicase C-terminal domain.
      Contains 2 PHD-type zinc fingers.
    • Cellular localization
      Nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • ATP-dependent helicase CHD5 antibody
      • CHD 5 antibody
      • CHD-5 antibody
      • CHD5 antibody
      • CHD5_HUMAN antibody
      • Chromodomain-helicase-DNA-binding protein 5 antibody
      • DKFZp434N231 antibody
      • KIAA0444 antibody
      • RP1-233K16.2 antibody
      see all

    Images

    • All lanes : Anti-Chromodomain helicase DNA binding protein 5 antibody [EPR6053] (ab124736) at 1/50000 dilution

      Lane 1 : Human adrenal gland cell lysate
      Lane 2 : SH-SY5Y cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-Rabbit HRP at 1/2000 dilution

      Predicted band size: 223 kDa

    • ab124736 at 1/250 dilution staining Chromodomain helicase DNA binding protein 5 in SH-SY5Y cells by Immunofluorescence.
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    ab124736 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (mouse neural stem cell)
    Gel Running Conditions
    Reduced Denaturing (4-12%)
    Loading amount
    40 µg
    Specification
    mouse neural stem cell
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Username

    Abcam user community

    Verified customer

    Submitted Jul 03 2018

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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