Anti-Chromogranin A antibody (ab17064)
Key features and details
- Rabbit polyclonal to Chromogranin A
- Suitable for: Sandwich ELISA, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Chromogranin A antibody
See all Chromogranin A primary antibodies -
Description
Rabbit polyclonal to Chromogranin A -
Host species
Rabbit -
Tested applications
Suitable for: Sandwich ELISA, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
C-terminal half of Chromogranin A, purified from urine of patients with carcinoid syndrome.
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Positive control
- Pancreas
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.3
Preservative: 0.05% Sodium azide
Constituent: 1% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab17064 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Sandwich ELISA |
Use a concentration of 0.5 µg/ml.
For sandwich ELISA, use this antibody as Detection with ab36997 as Capture. |
|
| IHC-P |
1/25 - 1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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| Notes |
|---|
|
Sandwich ELISA
Use a concentration of 0.5 µg/ml. For sandwich ELISA, use this antibody as Detection with ab36997 as Capture. |
|
IHC-P
1/25 - 1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
Target
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Function
Pancreastatin strongly inhibits glucose induced insulin release from the pancreas. -
Sequence similarities
Belongs to the chromogranin/secretogranin protein family. -
Post-translational
modificationsSulfated on tyrosine residues and/or contains sulfated glycans.
O-glycosylated with core 1 or possibly core 8 glycans. -
Cellular localization
Secreted. Neuroendocrine and endocrine secretory granules. - Information by UniProt
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Database links
- Entrez Gene: 1113 Human
- Omim: 118910 Human
- SwissProt: P10645 Human
- Unigene: 150793 Human
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Form
According to the Swiss-Prot database, this protein has many different forms which correspond to various molecular weights; thus the possible variation from the predicted molecular weight of 51kDa. -
Alternative names
- beta Granin antibody
- betagranin (N-terminal fragment of chromogranin A) antibody
- catestatin antibody
see all
Images
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Sandwich ELISA - Anti-Chromogranin A antibody (ab17064)
Standard Curve for Chromogranin A (Analyte: ab85486) dilution range 1pg/ml to 1ug/ml using Capture Antibody Mouse monoclonal [23A1] to Chromogranin A (ab36997) at 1ug/ml and Detector Antibody Rabbit polyclonal to Chromogranin A (ab17064) at 0.5ug/ml. Concentration of ab17064 may vary from lot to lot; please use this curve as guideline.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Chromogranin A antibody (ab17064)ab17064 staining Chromogranin A in formalin-fixed, paraffin-embedded Human pancreas tissue by Immunohistochemistry.
Datasheets and documents
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Datasheet download
References (3)
ab17064 has been referenced in 3 publications.
- Weis VG et al. Loss of MYO5B in mice recapitulates Microvillus Inclusion Disease and reveals an apical trafficking pathway distinct to neonatal duodenum. Cell Mol Gastroenterol Hepatol 2:131-157 (2016). PubMed: 27019864
- Qu X et al. Growth Factor Independence-1 (Gfi1) Is Required for Pancreatic Acinar Unit Formation and Centroacinar Cell Differentiation. Cell Mol Gastroenterol Hepatol 1:233-247.e1 (2015). PubMed: 28247862
- Saqui-Salces M et al. A high-fat diet regulates gastrin and acid secretion through primary cilia. FASEB J : (2012). IHC-P ; Mouse . PubMed: 22516298
