Anti-Chromogranin A antibody (ab17064)
- Datasheet
- References (3)
- Protocols
Overview
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Product nameAnti-Chromogranin A antibody
See all Chromogranin A primary antibodies -
DescriptionRabbit polyclonal to Chromogranin A
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Host speciesRabbit
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Tested applicationsSuitable for: Sandwich ELISA, IHC-Pmore details
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Species reactivityReacts with: Human
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Immunogen
C-terminal half of Chromogranin A, purified from urine of patients with carcinoid syndrome.
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Positive control
- Pancreas
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Storage bufferPreservative: 0.05% Sodium azide
Constituent: 1% BSA -
Concentration information loading... -
PurityProtein A purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab17064 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Sandwich ELISA | Use a concentration of 0.5 µg/ml. For sandwich ELISA, use this antibody as Detection with ab36997 as Capture. |
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| IHC-P | 1/25 - 1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
Target
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FunctionPancreastatin strongly inhibits glucose induced insulin release from the pancreas.
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Sequence similaritiesBelongs to the chromogranin/secretogranin protein family.
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Post-translational
modificationsSulfated on tyrosine residues and/or contains sulfated glycans.
O-glycosylated with core 1 or possibly core 8 glycans. -
Cellular localizationSecreted. Neuroendocrine and endocrine secretory granules.
- Information by UniProt
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Database links
- Entrez Gene: 1113 Human
- Omim: 118910 Human
- SwissProt: P10645 Human
- Unigene: 150793 Human
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FormAccording to the Swiss-Prot database, this protein has many different forms which correspond to various molecular weights; thus the possible variation from the predicted molecular weight of 51kDa.
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Alternative names
- beta Granin antibody
- betagranin (N-terminal fragment of chromogranin A) antibody
- catestatin antibody
see all
Images
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Sandwich ELISA - Anti-Chromogranin A antibody (ab17064)Standard Curve for Chromogranin A (Analyte: ab85486) dilution range 1pg/ml to 1ug/ml using Capture Antibody Mouse monoclonal [23A1] to Chromogranin A (ab36997) at 1ug/ml and Detector Antibody Rabbit polyclonal to Chromogranin A (ab17064) at 0.5ug/ml. Concentration of ab17064 may vary from lot to lot; please use this curve as guideline. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Chromogranin A antibody (ab17064)ab17064 staining Chromogranin A in formalin-fixed, paraffin-embedded Human pancreas tissue by Immunohistochemistry.
Datasheets and documents
References
This product has been referenced in:
- Weis VG et al. Loss of MYO5B in mice recapitulates Microvillus Inclusion Disease and reveals an apical trafficking pathway distinct to neonatal duodenum. Cell Mol Gastroenterol Hepatol 2:131-157 (2016). Read more (PubMed: 27019864) »
- Qu X et al. Growth Factor Independence-1 (Gfi1) Is Required for Pancreatic Acinar Unit Formation and Centroacinar Cell Differentiation. Cell Mol Gastroenterol Hepatol 1:233-247.e1 (2015). Read more (PubMed: 28247862) »
