Non-Abcam antibody was used: anti Rabbit IgG Host species: Goat Clonality: Polyclonal Conjugation: Biotin
Submitted image demonstrates my comment regarding distribution of positivity between Abs against the same protein: see my abreview on ab45138. Using this Ab, I obtain a diffuse, strong cytoplasmic positivity in Islet cell cytoplasm. NB: at 1/2000 dilution factor, I obtain the same pattern of immunostaining but, weaker. There is no apparent sub-localisation of positivity within the cytoplasm ( unlike sequential sections imunostained using ab15160 and ab45138) This Ab appears to be what is term "a very clean Ab" ( high signal:noise).
Mr. Carl Hobbs
Submitted Sep 06 2010
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