Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Chromogranin A antibody

Excellent
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Pancreas)
Specification
Pancreas
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid pH6
Permeabilization
No
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C

Other product details

Dilution
1/1000
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 21°C · Diluent: TBS/BSA/azide

Secondary antibody

Name
Non-Abcam antibody was used: anti Rabbit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Dilution
1/200

Additional data

Additional Notes
Submitted image demonstrates my comment regarding distribution of positivity between Abs against the same protein: see my abreview on ab45138.
Using this Ab, I obtain a diffuse, strong cytoplasmic positivity in Islet cell cytoplasm.
NB: at 1/2000 dilution factor, I obtain the same pattern of immunostaining but, weaker. There is no apparent sub-localisation of positivity within the cytoplasm ( unlike sequential sections imunostained using ab15160 and ab45138)
This Ab appears to be what is term "a very clean Ab" ( high signal:noise).

Mr. Carl Hobbs

Verified customer

Submitted Sep 06 2010

Sign up