Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Chromogranin A antibody

Good
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Dog Tissue sections (Small intestine)
Specification
Small intestine
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid pH6
Permeabilization
No
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C

Other product details

Dilution
1/500
Incubation time
2 hour(s) and 0 minute(s) · Temperature: 21°C · Diluent: TBS/BSA/azide

Secondary antibody

Name
Non-Abcam antibody was used: anti Rabbit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Dilution
1/200

Additional data

Additional Notes
Good demonstration of neuroendocrine cells.

Unless otherwise stated, all of my submitted DAB images are achieved using a manual stABCpx detection system.

Mr. Carl Hobbs

Verified customer

Submitted Sep 05 2011

Sign up