Key features and details
- Mouse monoclonal [CHGA/777] to Chromogranin A
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG1
Product nameAnti-Chromogranin A antibody [CHGA/777]
See all Chromogranin A primary antibodies
DescriptionMouse monoclonal [CHGA/777] to Chromogranin A
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Human
Does not react with: Rat
Recombinant full length protein corresponding to Human Chromogranin A.
Database link: P10645
- Human Panc-1 cell lysate;Human pheochromocytoma, pancreas and adrenal gland tissues.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.05% BSA
Concentration information loading...
PurityProtein A/G purified
Purification notesab220190 is purified from Bioreactor Concentrate by Protein A/G column chromatography.
Light chain typekappa
Our Abpromise guarantee covers the use of ab220190 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
For 30 minutes at RT.
|WB||Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 50 kDa.|
FunctionPancreastatin strongly inhibits glucose induced insulin release from the pancreas.
Sequence similaritiesBelongs to the chromogranin/secretogranin protein family.
modificationsSulfated on tyrosine residues and/or contains sulfated glycans.
O-glycosylated with core 1 or possibly core 8 glycans.
Cellular localizationSecreted. Neuroendocrine and endocrine secretory granules.
- Information by UniProt
FormAccording to the Swiss-Prot database, this protein has many different forms which correspond to various molecular weights; thus the possible variation from the predicted molecular weight of 51kDa.
- beta Granin antibody
- betagranin (N-terminal fragment of chromogranin A) antibody
- catestatin antibody
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human pheochromocytoma tissue labeling Chromogranin A with ab220190 at 1 µg/ml.
Anti-Chromogranin A antibody [CHGA/777] (ab220190) at 1 µg/ml + Panc-1 cell lysate
Predicted band size: 50 kDa
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human pancreas tissue labeling Chromogranin A with ab220190 at 1 µg/ml.
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human adrenal gland tissue labeling Chromogranin A with ab220190 at 1 µg/ml.
ab220190 has not yet been referenced specifically in any publications.