Recombinant
RabMAb

Anti-Chromogranin A antibody [EP1030Y] (ab68271)

Submit an Abreview Q&A (1)Specific References (2)

Overview

  • Product name
    Anti-Chromogranin A antibody [EP1030Y]
    See all Chromogranin A primary antibodies
  • Description
    Rabbit monoclonal [EP1030Y] to Chromogranin A
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC, Flow Cytmore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human Chromogranin A (N terminal). The exact sequence is proprietary.

  • Positive control
    • WB: SH-SY5Y cell lysate. WB: Human pancreas tissue.
  • General notes

    A trial size is available to purchase for this antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab68271 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Detects a band of approximately 90 kDa (predicted molecular weight: 51 kDa).
IHC-P 1/100 - 1/250.
ICC 1/100 - 1/250.
Flow Cyt 1/20.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
  • Application notes
    Is unsuitable for IP.

    • Target

    • Function
      Pancreastatin strongly inhibits glucose induced insulin release from the pancreas.
    • Sequence similarities
      Belongs to the chromogranin/secretogranin protein family.
    • Post-translational
      modifications
      Sulfated on tyrosine residues and/or contains sulfated glycans.
      O-glycosylated with core 1 or possibly core 8 glycans.
    • Cellular localization
      Secreted. Neuroendocrine and endocrine secretory granules.
    • Information by UniProt
    • Database links
      • Form
        According to the Swiss-Prot database, this protein has many different forms which correspond to various molecular weights; thus the possible variation from the predicted molecular weight of 51kDa.
      • Alternative names
        • beta Granin antibody
        • betagranin (N-terminal fragment of chromogranin A) antibody
        • catestatin antibody
        • CgA antibody
        • CHG A antibody
        • Chga antibody
        • chromofungin antibody
        • Chromogranin A antibody
        • Chromogranin A parathyroid secretory protein 1 antibody
        • Chromogranin A precursor antibody
        • ChromograninA antibody
        • CMGA_HUMAN antibody
        • ER-37 antibody
        • Pancreastatin antibody
        • Parastatin antibody
        • Parathyroid secretory protein 1 antibody
        • Pituitary secretory protein I antibody
        • Secretory protein I antibody
        • SP I antibody
        • SP-I antibody
        • SP1 antibody
        • SPI antibody
        • Vasostatin antibody
        • Vasostatin I antibody
        • Vasostatin II antibody
        see all

      Images

      • Western blot - Anti-Chromogranin A antibody [EP1030Y] (ab68271)
        Western blot - Anti-Chromogranin A antibody [EP1030Y] (ab68271)
        Anti-Chromogranin A antibody [EP1030Y] (ab68271) + SH-SY5Y (Human neuroblastoma cell line from bone marrow) cell lysate at 10 µg

        Secondary
        HRP conjugated Goat anti-rabbit at 1/2000 dilution

        Predicted band size: 51 kDa
        Observed band size: 90 kDa (why is the actual band size different from the predicted?)

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Chromogranin A antibody [EP1030Y] (ab68271)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Chromogranin A antibody [EP1030Y] (ab68271)

        Formalin/PFA-fixed paraffin-embedded human pancreas tissue stained for Chromogranin A with ab68271 at a 1/100 dilution in immunohistochemical analysis.

      References

      This product has been referenced in:
      • Nakata R  et al. Contribution of neuroblastoma-derived exosomes to the production of pro-tumorigenic signals by bone marrow mesenchymal stromal cells. J Extracell Vesicles 6:1332941 (2017). Read more (PubMed: 28717423) »
      • Yamamoto Y  et al. Strong antitumor efficacy of a pancreatic tumor-targeting oncolytic adenovirus for neuroendocrine tumors. Cancer Med 6:2385-2397 (2017). Flow Cyt . Read more (PubMed: 28941156) »

      See all 2 Publications for this product

      Publishing research using ab68271? Please let us know so that we can cite the reference in this datasheet.

      Customer reviews and Q&As

      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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