Product nameAnti-Chromogranin A antibody [EP1030Y] (Alexa Fluor® 488)
See all Chromogranin A primary antibodies
DescriptionRabbit monoclonal [EP1030Y] to Chromogranin A (Alexa Fluor® 488)
ConjugationAlexa Fluor® 488. Ex: 495nm, Em: 519nm
Tested applicationsSuitable for: Flow Cytmore details
Species reactivityReacts with: Human
Synthetic peptide within Human Chromogranin A (N terminal). The exact sequence is proprietary.
- Flow Cytometry: SH-SY5Y cells
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or email@example.com.
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. Store In the Dark.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 30% Glycerol, 1% BSA, PBS
Concentration information loading...
PurityProtein A purified
- Anti-Chromogranin A antibody [EP1030Y] (HRP) (ab199194)
- Anti-Chromogranin A antibody [EP1030Y] (Phycoerythrin) (ab213341)
- Anti-Chromogranin A antibody [EP1030Y] (Alexa Fluor® 568) (ab214399)
- Anti-Chromogranin A antibody [EP1030Y] - BSA and Azide free (ab215276)
- Anti-Chromogranin A antibody [EP1030Y] (ab68271)
Our Abpromise guarantee covers the use of ab199192 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
FunctionPancreastatin strongly inhibits glucose induced insulin release from the pancreas.
Sequence similaritiesBelongs to the chromogranin/secretogranin protein family.
modificationsSulfated on tyrosine residues and/or contains sulfated glycans.
O-glycosylated with core 1 or possibly core 8 glycans.
Cellular localizationSecreted. Neuroendocrine and endocrine secretory granules.
- Information by UniProt
FormAccording to the Swiss-Prot database, this protein has many different forms which correspond to various molecular weights; thus the possible variation from the predicted molecular weight of 51kDa.
- beta Granin antibody
- betagranin (N-terminal fragment of chromogranin A) antibody
- catestatin antibody
Overlay histogram showing SHSY5Y cells stained with ab199192 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab199192, 1/50 dilution) for 30 min at 22°C.
Isotype control antibody (black line) was rabbit IgG (monoclonal) AF488 (ab199091) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.
Acquisition of >5,000 events were collected using a 50mW Blue laser (488nm) and 530/30 bandpass filter.
This antibody gave a positive signal in SHSY5Y cells fixed with 4% formaldehyde (10 min)/permeabilized with 0.1% PBS-Triton X-100 for 15 min used under the same conditions.
ab199192 has not yet been referenced specifically in any publications.