Product nameAnti-Chromogranin A antibody [EP1030Y] (Alexa Fluor® 568)
See all Chromogranin A primary antibodies
DescriptionRabbit monoclonal [EP1030Y] to Chromogranin A (Alexa Fluor® 568)
ConjugationAlexa Fluor® 568. Ex: 578nm, Em: 603nm
Tested applicationsSuitable for: IHC-Frmore details
Species reactivityReacts with: Human
Synthetic peptide within Human Chromogranin A aa 1-100 (N terminal). The exact sequence is proprietary.
Database link: P10645
- IHC-Fr: normal human pancreas tissue sections
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or email@example.com.
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. Store In the Dark.
Storage bufferpH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 30% Glycerol, 1% BSA
Concentration information loading...
PurityProtein A purified
- Anti-Chromogranin A antibody [EP1030Y] (Alexa Fluor® 488) (ab199192)
- Anti-Chromogranin A antibody [EP1030Y] (HRP) (ab199194)
- Anti-Chromogranin A antibody [EP1030Y] (Phycoerythrin) (ab213341)
- Anti-Chromogranin A antibody [EP1030Y] - BSA and Azide free (ab215276)
- Anti-Chromogranin A antibody [EP1030Y] (ab68271)
Our Abpromise guarantee covers the use of ab214399 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
This product gave a positive signal in frozen human pancreas tissue fixed with 10% formaldehyde (10 min)
FunctionPancreastatin strongly inhibits glucose induced insulin release from the pancreas.
Sequence similaritiesBelongs to the chromogranin/secretogranin protein family.
modificationsSulfated on tyrosine residues and/or contains sulfated glycans.
O-glycosylated with core 1 or possibly core 8 glycans.
Cellular localizationSecreted. Neuroendocrine and endocrine secretory granules.
- Information by UniProt
FormAccording to the Swiss-Prot database, this protein has many different forms which correspond to various molecular weights; thus the possible variation from the predicted molecular weight of 51kDa.
- beta Granin antibody
- betagranin (N-terminal fragment of chromogranin A) antibody
- catestatin antibody
IHC image of Chromogranin A staining in a section of frozen normal human pancreas*.
The section was fixed using 10% formaldehyde in 1XPBS for 10 minutes. No antigen retrieval step was performed prior to staining. Non-specific protein-protein interactions were then blocked in TBS containing 0.025% (v/v) Triton X-100, 0.3M (w/v) glycine and 1% (w/v) BSA for 1h at room temperature. The section was then incubated overnight at +4°C in TBS containing 0.025% (v/v) Triton X-100 and 1% (w/v) BSA with ab214399 at 1/100 dilution (pseudocolored in green) and counterstained using ab195884, Rat monoclonal to Tubulin (Alexa Fluor® 647), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Fluoromount®.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.
ab214399 has not yet been referenced specifically in any publications.