Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP1031Y] to Chromogranin A
- Suitable for: WB, IHC-P
- Reacts with: Rat, Human
Product nameAnti-Chromogranin A antibody [EP1031Y]
See all Chromogranin A primary antibodies
DescriptionRabbit monoclonal [EP1031Y] to Chromogranin A
Tested applicationsSuitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt or ICC
Species reactivityReacts with: Rat, Human
Synthetic peptide within Human Chromogranin A (C terminal). The exact sequence is proprietary.
(Peptide available as
- PC12 cytoplasmic cell lysate Human pancreatic tissue
Mouse: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Dissociation constant (KD)KD = 6.90 x 10 -12 M Learn more about KD
Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab52983 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1e+006. Detects a band of approximately 80-90 kDa (predicted molecular weight: 51 kDa).|
|IHC-P||1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
FunctionPancreastatin strongly inhibits glucose induced insulin release from the pancreas.
Sequence similaritiesBelongs to the chromogranin/secretogranin protein family.
modificationsSulfated on tyrosine residues and/or contains sulfated glycans.
O-glycosylated with core 1 or possibly core 8 glycans.
Cellular localizationSecreted. Neuroendocrine and endocrine secretory granules.
- Information by UniProt
FormAccording to the Swiss-Prot database, this protein has many different forms which correspond to various molecular weights; thus the possible variation from the predicted molecular weight of 51kDa.
- beta Granin antibody
- betagranin (N-terminal fragment of chromogranin A) antibody
- catestatin antibody
ab52983, at a 1/250 dilution, staining Human Chromogranin A in Pancreas using Immunohistochemistry, Paraffin Emebedded tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Anti-Chromogranin A antibody [EP1031Y] (ab52983) at 1/1000000 dilution + PC12 cell lysate at 10 µg
goat anti-rabbit HRP labelled at 1/2000 dilution
Predicted band size: 51 kDa
Observed band size: 80-90 kDa why is the actual band size different from the predicted?
Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KD
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab52983 has been referenced in 2 publications.
- Thomsen SK et al. Type 2 diabetes risk alleles in PAM impact insulin release from human pancreatic ß-cells. Nat Genet 50:1122-1131 (2018). PubMed: 30054598
- Simpson PD et al. Striking Oxygen Sensitivity of the Peptidylglycine a-Amidating Monooxygenase (PAM) in Neuroendocrine Cells. J Biol Chem 290:24891-901 (2015). WB ; Human . PubMed: 26296884