Overview

  • Product name

    Anti-Chromogranin A antibody [LK2H10 + PHE5 + CGA414]
    See all Chromogranin A primary antibodies
  • Description

    Mouse monoclonal [LK2H10 + PHE5 + CGA414] to Chromogranin A
  • Host species

    Mouse
  • Tested applications

    Suitable for: ICC/IF, Flow Cyt, IP, IHC-P, WB, IHC-Frmore details
  • Species reactivity

    Reacts with: Rat, Human, Pig, Monkey
  • Immunogen

    This product was produced with the following immunogens:
    Recombinant full length protein corresponding to Human Chromogranin A. (CGA414)
    Database link: P10645

    Tissue, cells or virus corresponding to Human Chromogranin A. Human pheochromocytoma. (LK2H10 and PHE5)

  • Positive control

    • PC12 cells. Adrenal gland, bowel, thyroid, pancreas or pheochromocytoma.
  • General notes

    Please note that this antibody is an oligoclonal antibody. It is a cocktail of monoclonal antibodies that have been carefully selected. Oligoclonal antibodies have not only the specificity and batch-to-batch consistency of a monoclonal antibody, but also have the advantage of the sensitivity of a polyclonal antibody due to their ability to recognize multiple epitopes on an antigen. 

Properties

Applications

Our Abpromise guarantee covers the use of ab199014 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 2 µg/ml.
Flow Cyt Use 0.5-1µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

IP Use at 2-4 µg/mg of lysate.
IHC-P Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

30 minutes at RT.

WB Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 68-75 kDa.
IHC-Fr Use a concentration of 0.5 - 1 µg/ml.

30 minutes at RT.

Target

  • Function

    Pancreastatin strongly inhibits glucose induced insulin release from the pancreas.
  • Sequence similarities

    Belongs to the chromogranin/secretogranin protein family.
  • Post-translational
    modifications

    Sulfated on tyrosine residues and/or contains sulfated glycans.
    O-glycosylated with core 1 or possibly core 8 glycans.
  • Cellular localization

    Secreted. Neuroendocrine and endocrine secretory granules.
  • Information by UniProt
  • Database links

  • Form

    According to the Swiss-Prot database, this protein has many different forms which correspond to various molecular weights; thus the possible variation from the predicted molecular weight of 51kDa.
  • Alternative names

    • beta Granin antibody
    • betagranin (N-terminal fragment of chromogranin A) antibody
    • catestatin antibody
    • CgA antibody
    • CHG A antibody
    • Chga antibody
    • chromofungin antibody
    • Chromogranin A antibody
    • Chromogranin A parathyroid secretory protein 1 antibody
    • Chromogranin A precursor antibody
    • ChromograninA antibody
    • CMGA_HUMAN antibody
    • ER-37 antibody
    • Pancreastatin antibody
    • Parastatin antibody
    • Parathyroid secretory protein 1 antibody
    • Pituitary secretory protein I antibody
    • Secretory protein I antibody
    • SP I antibody
    • SP-I antibody
    • SP1 antibody
    • SPI antibody
    • vasostatin 2 antibody
    • Vasostatin antibody
    • Vasostatin I antibody
    • Vasostatin II antibody
    • vasostatin-2 antibody
    see all

Images

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded human small cell lung carcinoma labeling Chromogranin A with ab199014 at 1 µg/mL.

References

This product has been referenced in:

  • Saraf MK  et al. Formula diet driven microbiota shifts tryptophan metabolism from serotonin to tryptamine in neonatal porcine colon?. Microbiome 5:77 (2017). Read more (PubMed: 28705171) »
See 1 Publication for this product

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